Dynamic host configuration protocol (DHCP) is a protocol for managing and assigning IP addresses and is widely used in enterprise networks. Considering the problems of students’ lack of practical ability and the limited hardware of computer network equipment in the learning process, this paper proposes a kind of integrated use of eNSP, VirtualBox and Kali software to build a DHCP multi-level experimental project. The design of the experiment project covers the configuration of common DHCP servers, DHCP relay, and DHCP attack and defense. The design of this experimental project can improve students’ practical ability of network engineering, facilitate teachers to carry out online teaching, and improve teaching quality.

How to adjust the flow cell sorter for the sensitive cells is a concern of many practitioners. Using the FACSAria Fusion as an example, the article creatively combined the service interface with the user interface to adjust the lowest default sample pressure and then sorted the primary naive T cells according to the standard user’s manual with the 85μm nozzle. The testing quality of the purified naive T cells revealed that the procedure could lead to high purity products. Moreover, treg cells differentiated from the products could generate Foxp3 normally compared with the non-sorting control.

“Data structure” is an important course in the field of software design, and it is particularly important for students majoring in measurement and control technology to master the knowledge of data structure. However, in the teaching process, how to combine the general data structure knowledge with the practical engineering problems faced by the measurement and control technology major, and how to choose the appropriate nonlinear structure problem cases, so that students can deepen the grasp of the course content in both knowledge understanding and practical application, are the key problems to be solved in the teaching. Therefore, in the teaching practice, taking the automatic test command analysis program commonly used in the field of measurement and control technology research as an example, the nonlinear data structure algorithm experiment is designed. Through the integration of science and education, the depth and breadth of teaching are expanded, so that students can combine theoretical knowledge with practical skills, and effectively improve their ability to solve practical engineering problems.

In the direction of arrival (DOA) estimation, when the unit spacing of the receiving antenna array is greater than the half wavelength of the incident signal carrier, the manifold ambiguity will occur, which poses a challenge to distinguish the real signal DOA. Based on a subarray level large spacing uniform dual-polarization array, this article proposes to randomly change the polarization state of a certain unit in each subarray, so that the corresponding steering vectors of different subarrays are independent of each other. It can break the linearity between the steering vectors under a large spacing uniform array and suppress manifold ambiguity. The simulation and experimental results show that this method can effectively remove spurious peaks in the spatial spectrum of the multiple signal classification (MUSIC) algorithm and achieve a high-precision DOA estimation.

This article improves the measurements of liquid viscosity coefficient with falling ball and gives an intensive study. We briefly introduce the experimental steps for measuring the viscosity coefficient of liquids using video analysis. It employs the open-source software Kinovea to record and track the falling process of a ball, excluding any unqualified experimental procedures and analyzing qualified experiments frame by frame. This method enhances the experimental measurement accuracy to 240 frames per second and reduces experimental error by at least 50%. Moreover we intensively study the effects of size and edge on the experimental results.

A laboratory low vacuum-cooling water circulation system has been developed combined with the characteristics and existing problems of low vacuum and cooling water devices. Through reasonable installation arrangement and ingenious equipment selection, the dual functions of low vacuum and cooling water circulation have been realized with one fluid transporting equipment and the vacuum ejector based on Bernoulli equation principle. The equipment performance has been further improved. The equipment operation cost (about 40%) and occupied area have been reduced effectively. The operation safety of the basic laboratory has been improved effectively. The equipment operation risk has been reduced from the root, the operation safety has been improved, and the water recycling has achieved the goal of energy saving and emission reduction.

In order to solve the problem that it is difficult to measure the internal temperature and pressure parameters of large size samples in the current in-situ environmental simulation test of deep earth, a sensor sealing device applied to the geotechnical mechanics test system is designed. By prefabricating the device inside the sample and sealing the sensor components, direct contact between the sensor, the back-end wire and the high-temperature and high-pressure environment is avoided, and accurate measurement of temperature and pressure parameters inside the sample can be achieved. The verification test shows that the addition of this device does not disrupt the experimental environment where temperature, pressure, and oil coexist inside the triaxial chamber. The proposed device not only provides a guarantee for stable measurement of large-sized specimen mechanical tests, but also improves the accuracy and reliability of measurement. Due to the good sealing performance and resistance to high temperature and high pressure conditions of the device, slight improvements can be made and it can be used for operations in complex environments such as deep sea and deep space, which has broad application prospects.

In order to enhance students’ Innovation literacy and integrated scientific research content into experimental teaching, this work designs “experiments on the preparation and optical band gap control of ultrathin amorphous oxide semiconductor films”. InSnO films were fabricated on quartz substrates by magnetron sputtering, and several characterization methods were used to study the effects of thickness on the crystal structure, surface topography and optical band gap of the films. The results show that the thickness of film affects the surface roughness of the film, and the optical band gap of the films decreases with increasing film thickness. This innovative experiment covers material preparation, characterization and mechanism analysis, involving semiconductor, materials science, optoelectronics disciplines, and combined with actual scientific research content, which improves students’ enthusiasm for scientific research, cultivate students’ innovative thinking, and improves their ability to combine theory with practice.

With strong theoretical and practical significance, the principles and applications of enzymology are a key and difficult content in the course of biochemistry. In order to improve students’ comprehensive understanding and application ability of enzyme knowledge, a comprehensive experimental project “immobilization and isoenzyme identification of peroxidases” is designed. It integrates the main content of enzymes and focuses on five sections, namely, enzyme preparation, activity determination, characterization of enzymatic properties (Michaelis constant and stability), immobilization, and isoenzyme identification. Students’ ability to analyze and solve problems are improved by deep participation and coordinated implementation, and the experiment achieves a good teaching effect.

In view of the disadvantages of the traditional experimental teaching mode, such as outdated experimental teaching methods, insufficient subjective initiative of students, floating experimental report in form, simple final examination system and not highlighting the practically of Python programming language, this paper discusses the practical and effective experimental teaching mode of Python programming language that is in line with students’ reality. The experimental teaching mode of Python programming language takes video simulation + task driven as the experimental teaching method, requiring students to achieve “clear” experimental tasks and regular large assignments, and incorporate all learning tasks into the final examination system, so as to fully encourage students to write Python programs. At the same time, in view of the problems encountered in the implementation of the experimental teaching mode of the Python programming language, this paper gives some suggestions on the teaching self-examination, such as the team leader rotation system and the assistant teaching system, which further improves the smooth connection of the experimental teaching of Python programming language. Finally, based on the increasing difficulty of experimental tasks in the course of Machine Learning, the application of Python programming language in the field of artificial intelligence will be promoted.

The imperfection of the traditional substation simulation system has led to the limitations of substation practice teaching, and a new substation virtual simulation experiment teaching platform is developed based on the virtual reality technology. The virtual simulation experimental environment is based on the domestic 220 kV substation, and the design architecture of the system is optimized. In order to enable students to better understand and master the relevant knowledge of substation, and combine theoretical knowledge with practical operation, the functions of theoretical knowledge answering, fault simulation and processing are added. Experimental results show that compared with the traditional virtual teaching system, the operation is more convenient and the sense of immersion is stronger, which has great advantages in cultivating students’ innovative thinking ability.

In order to meet the need of training innovative, compound and application-oriented talents in the new era, the teaching reform of the course of experimental mechanics has been carried out. On the basis of a large number of real test data, a series of provincial and university-level first-class courses of virtual simulation experiments have been developed with the help of the information technology. At the same time, ideological and political elements, engineering application cases and cutting-edge research achievements related to the teaching contents have been integrated into the construction practice of the hybrid online and offline first-class course of experimental mechanics, which has improved the teaching quality and effect. The teaching reform can support the cultivation of high-quality innovative mechanics talents more effectively.

Based on the student-centered and training target-oriented teaching philosophy, centering on the construction of the curriculum system of step-by-step improvement, the course of Basic Experiment of Material Science has implemented a comprehensive innovation, including the transformation of teaching ideas, the optimization of teaching content and the enrichment of teaching methods. The characteristic experimental module integrated with scientific research and innovation elements is developed, combining online resources with offline teaching and coordinating the open experimental teaching mode, which promotes the achievement of the course objectives and the cultivation of innovative talents in the major of materials science and engineering.

According to some research achievements, a green oximation comprehensive experiment was designed to overcome the shortcomings of the traditional oximation reaction, and the base and reaction equipment were improved. Recyclable nano-K2CO3 was prepared firstly by ultrafine wet milling and characterized by the laser particle size analyzer, scanning electronic microscopy (SEM), and CO2-TPD. The synthesis of ethyl α-(hydroxyimino)benzenepropanoate was performed using nano-K2CO3 as the base. The preparation of ethyl nitrite and oximation of diethyl benzylmalonate were carried out simultaneously by series devices. The experiment avoids the shortcomings of sodium alkoxides that cannot be recovered and corrodes equipment, and solves the problems of storage and transportation difficulties and easy environmental pollution of volatile nitrite. This comprehensive experiment is beneficial for students to learn the idea of environment-friendly green chemistry, improve the students’ comprehensive ability and quality, and cultivate their scientific research innovation thinking ability.

By integrating the academic foreland and scientific research achievements into experimental teaching, it enhances and updates the existing teaching contents. This approach is crucial for connecting teaching with research, fostering students’ innovative skills, and nurturing their overall development. Through the integration of science and education, we have designed a research-based comprehensive chemical experiment titled “Synthesis and Characterization of Nano-sized Magnesium Oxide”. In the project design process, we introduced a multi-functional microwave synthesis instrument. By utilizing characterization technologies such as X-ray diffraction and transmission electron microscopy, we explored various reaction conditions including reaction duration and reactant proportion to optimize a suitable reaction scheme for the undergraduate experimental classroom teaching. The project involves multidisciplinary knowledge points that train students to analyze and solve problems by comprehensively using interdisciplinary knowledge, and improves their innovative thinking abilities and overall qualities.

To solve the increasing problem of water pollution, based on the cutting-edge research in the photocatalytic degradation of organic compounds, this paper designs a cross disciplinary comprehensive experiment on the efficient removal of organic pollutants by nanocomposite membranes based on the synergistic effect of adsorption and photocatalysis. Polymer p (MMA-AA) with carboxylate groups was experimentally synthesized and composite with PES and TiO2 to form a flat membrane. In an environment without light, relying solely on the adsorption of membranes has a lower efficiency in removing pollutants. Under the action of light, carboxylate groups continuously adsorb Rhodamine B through electrostatic adsorption, and are simultaneously degraded by TiO2 through photocatalysis, achieving rapid removal of pollutants.

Combined with the research hotspot of material, chemistry and life science, a comprehensive experiment on the preparation, characterization and biological properties of single element sulfur quantum dots was designed. Polyethylene glycol modified sulfur quantum dots (PEG-SQDs) were obtained by the top-down method. The morphology, elements, functional groups, particle size, potential and fluorescence properties of PEG-SQDs were comprehensively characterized and analyzed by various characterization methods. Meanwhile, the biocompatibility, fluorescence imaging ability and cell entry behavior of PEG-SQDs were also studied. This experiment includes several steps such as the preparation, characterization and biological property analysis of quantum dots, involving the basic knowledge of materials, chemistry and life science. It not only expands students’ scope of knowledge, but also helps to cultivate students’ comprehensive practical ability and problem-analyzing and solving ability.

A new fine-grained protocol analysis experiment teaching method based on Scapy is proposed to address the problems of coarse analysis granularity and poor teaching effects due to the interoperation between network applications in traditional protocol analysis experiments. Using Scapy, students can control the construction and sending sequence of protocol data units, making it a fine-grained network protocol analysis method as the interoperation unit in a packet. Taking the experimental project “TCP connection management” as an example, this paper describes in detail how to implement the fine-grained protocol analysis experiment teaching with Scapy. The teaching practice demonstrates that applying Scapy to the experimental teaching of protocol analysis encourages students to think deeply about network protocols through the packet interaction of both sides, resulting in remarkable teaching effects.

In order to address the deficiencies in the evaluation of the practical teaching performance in colleges and universities, this paper applies the ENTROPY-TOPSIS algorithm based on the modification to the experimental course performance evaluation, and deduces the principle of the specific algorithm. At the same time, the algorithm and the commonly used weighting algorithm hierarchical analysis method and the CRITIC algorithm in the circuit practice results in the process of objective weighting for horizontal comparison. The results show that the method can provide a theoretical basis for the scientific assignment of multi-source heterogeneous evaluation data, effectively eliminating the irrationality due to subjective factors, and making teachers more objective and scientific in the assessment of experimental grades.

Targeting regulatory enzymes is an important aspect of chemical biology. The approach involves acquiring target enzymes through heterologous expression, studying their properties, and ultimately applying them in practical settings. Microcystinase A (MlrA) was identified and successfully prepared and characterized as a target enzyme. The study analyzed both the purity of the enzyme and its resulting effects, examining how different substrate concentrations, temperature, pH, and metal ions affected MlrA’s activity in microcystins (MCs) degradation. Through experimentation and exploration, students can learn to prepare and analyze target proteins, design experiments to explore enzyme activity under different conditions, and use key instruments, which improves their problem-solving skills, their ability to apply chemical biology knowledge, and their scientific inquiry and social responsibility. In the process of experimental teaching, students were guided to think about the value and significance of scientific research to society, realize the importance of scientific ethics and social responsibility, and understand the social responsibility and mission of scientific researchers, which cunltivate their senses of scientific inquiry and social responsibility.

Taking 4-bromochalcone as the target molecule, through the retrosynthetic analysis, the synthesis routes containing 5 Nobel Prize achievements were analyzed from 6 routes. Through comparison, the 2021 Nobel Prize achievement “Pure Organic Catalysis” was finally selected to synthesize 4-bromochalcone at room temperature. A new reagent L-proline was used as a catalyst to synthesize 4-bromochalcone by the aldol condensation of p-bromobenzaldehyde and acetophenone at room temperature. A series of orthogonal experiments were carried out to optimize the reaction conditions. The reaction has the advantages of low cost of raw materials and mild reaction conditions. Through simple reaction, a number of Nobel Prize achievements and pure organic catalysis are introduced into experimental teaching, avoiding “prescribed medicine” teaching, keeping close to the frontier of science, and deepening the study of organic synthesis, which is conducive to cultivating students’ innovative consciousness.

With the “Healthy China 2030” Plan, research on high-end medical devices is strongly supported by our country. positron emission tomography (PET)/computed tomography (CT) is one of the most complex and highest value medical imaging devices in the world. However, it is difficult to carry out the teaching and training of PET/CT, due to the adverse factors of complex equipment operation, expensive price and high cost of offline training. In this study, a PET/CT virtual simulation experiment platform was developed based on the virtual reality technology. The proposed system includes the functions of virtual PET/CT department scene roaming, imaging principle, instrument structure and the assessment and evaluation of instrument operations. The target users of the platform are students of biomedical engineering, medical imaging and other related majors. Based on this platform, students can learn the knowledge, principles and usage of PET/CT anytime and anywhere, regardless of time and space. The interactive functions featured by the platform can also better enhance students’ engagement and desire for exploration, achieving more desirable teaching and learning results.

The electron microscope public laboratory of “Materials Testing and Analysis Center” is a university-level registered laboratory with public service based on the “double first-class” construction of materials science and engineering in Shanghai Jiao Tong University. After years of laboratory opening, an efficient, standardized and normalized management operation mode has been gradually formed from the testing and analysis service, supporting experimental teaching to experimental innovation and development. On the one hand, the laboratory can provide high-quality test and analysis services, ensure high-quality experimental data, strongly support the experimental research of university research teams, and help the technical cooperation of off-campus units. On the other hand, the laboratory undertakes the cultivation of high-quality talents and plays an important role in the experimental technology and equipment training, experimental teaching construction and reform, practical technical guidance and analysis support. In addition, combined with the key management and technical problems of public laboratory, innovative experimental management methods, standardized experimental process and equipment function development have improved the efficiency of laboratory opening up and the service ability of technicians.

According to the real-time, portability and economic needs of the pedestrian positioning system, the paper designs and builds an experimental platform about real-time inertial navigation of pedestrian based on the system on chip (SoC). The platform has both rich computing resources and rich interface resources. Without relying on any external auxiliary equipment, the platform displays the position of pedestrians in different sports modes in real time. Compared with the traditional inertial navigation positioning platform, the platform can expand multiple comprehensive and exploratory experiments, and support basic or professional experiments of different stages and different difficulties. Compared with the common experiment platform of neural network, the platform performs better in terms of cost, power consumption, volume, etc. The experiment platform with both real-time and portability can provide intuitive and thorough practical experience for students. Compared with the simulation verification, the new experiment platform help stimulate students’ interest in learning, and balance the cultivation between scientific research exploration ability and engineering realization ability.

At present, the national standard for the construction of university laboratories has not yet been issued. There are few reports on the practice of the hardware construction of teaching laboratory and that more details need to be paid attention to the process of the hardware construction of teaching laboratories. Based on the nature and characteristics of the discipline, the Polymer Chemistry Laboratory of the School of Chemistry at Jilin University, with the main themes of applicability, practicality, and safety, shows the specific process of standardized basic hardware construction for synthetic teaching laboratories in the field of chemistry, which provides a useful reference for the introduction of laboratory construction standards in colleges and universities.

The investment of instrument and equipment in colleges and universities is increasing continuously. How to manage and control the instruments and equipment well so as to make them play a full role in the teaching and scientific research is a problem that the majority of universities instruments and equipment management workers try to solve. After a long period of exploration and practice, Sichuan University has proposed the 10S refined instrument and equipment management system for the first time. Promoting development through management, combining theory with practice, and combining the standardization and modernization of the daily management of instruments and equipment have greatly improved the scientific and refined level of the management of instruments and equipment, and effectively improved the benefits of the opening and sharing of instruments and equipment. The specific content of 10S fine management in the instrument and equipment management is expounded and analyzed, which can provide some reference and help for the instrument and equipment management in other universities.

Basing on pain points of development and market demands, we define the function and orientation of the shared experimental platform for the medical device around translational medicine. In order to ensure the sustainable and efficient operation, hardware and software construction scheme and unique mechanism for efficient operation open sharing were established, focusing on the construction goal of translational medicine infrastructure and the science base of our university. The goals of serving scientific research and helping innovation were completed.