How to adjust the flow cell sorter for the sensitive cells is a concern of many practitioners. Using the FACSAria Fusion as an example, the article creatively combined the service interface with the user interface to adjust the lowest default sample pressure and then sorted the primary naive T cells according to the standard user’s manual with the 85μm nozzle. The testing quality of the purified naive T cells revealed that the procedure could lead to high purity products. Moreover, treg cells differentiated from the products could generate Foxp3 normally compared with the non-sorting control.