A laboratory low vacuum-cooling water circulation system has been developed combined with the characteristics and existing problems of low vacuum and cooling water devices. Through reasonable installation arrangement and ingenious equipment selection, the dual functions of low vacuum and cooling water circulation have been realized with one fluid transporting equipment and the vacuum ejector based on Bernoulli equation principle. The equipment performance has been further improved. The equipment operation cost (about 40%) and occupied area have been reduced effectively. The operation safety of the basic laboratory has been improved effectively. The equipment operation risk has been reduced from the root, the operation safety has been improved, and the water recycling has achieved the goal of energy saving and emission reduction.