With the “Healthy China 2030” Plan, research on high-end medical devices is strongly supported by our country. positron emission tomography (PET)/computed tomography (CT) is one of the most complex and highest value medical imaging devices in the world. However, it is difficult to carry out the teaching and training of PET/CT, due to the adverse factors of complex equipment operation, expensive price and high cost of offline training. In this study, a PET/CT virtual simulation experiment platform was developed based on the virtual reality technology. The proposed system includes the functions of virtual PET/CT department scene roaming, imaging principle, instrument structure and the assessment and evaluation of instrument operations. The target users of the platform are students of biomedical engineering, medical imaging and other related majors. Based on this platform, students can learn the knowledge, principles and usage of PET/CT anytime and anywhere, regardless of time and space. The interactive functions featured by the platform can also better enhance students’ engagement and desire for exploration, achieving more desirable teaching and learning results.