In view of the disadvantages of the traditional experimental teaching mode, such as outdated experimental teaching methods, insufficient subjective initiative of students, floating experimental report in form, simple final examination system and not highlighting the practically of Python programming language, this paper discusses the practical and effective experimental teaching mode of Python programming language that is in line with students’ reality. The experimental teaching mode of Python programming language takes video simulation + task driven as the experimental teaching method, requiring students to achieve “clear” experimental tasks and regular large assignments, and incorporate all learning tasks into the final examination system, so as to fully encourage students to write Python programs. At the same time, in view of the problems encountered in the implementation of the experimental teaching mode of the Python programming language, this paper gives some suggestions on the teaching self-examination, such as the team leader rotation system and the assistant teaching system, which further improves the smooth connection of the experimental teaching of Python programming language. Finally, based on the increasing difficulty of experimental tasks in the course of Machine Learning, the application of Python programming language in the field of artificial intelligence will be promoted.