With the development of ultra-weak bioluminescence detection technology, more and more scholars have applied this technology to medical researches. Through analysis and summary, this paper found that ultra-weak bioluminescence can not only assist in disease diagnosis and evaluation of human skin status in modern medicine, but also be used in acupuncture and moxibustion, property of traditional Chinese medicine and medical treatment of TCM syndrome type. In addition, other parameters besides photon intensity involving Q value, compressed state parameter (SSI) and Fano factor, are introduced, and the changes of ultra-weak bioluminescence during emotion, motion and death are also summarized. Ultra-weak bioluminescence is widely used in medical field and has strong practicability. In order to have a more detailed understanding of ultra-weak bioluminescence in human body and show the development potential of ultra-weak bioluminescence in the medical field, this paper made a comprehensire review on the subject.

Photon therapy has been widely used in the clinical treatment of pain diseases because of its safety, noninvasive, painless, non-invasive, non addictive, convenient operation and remarkable curative effects.?This paper introduces the research progress of low-level laser therapy, high-intensity laser therapy, semiconductor lase therapy and laser acupuncture therapy based on photon technology in pain relief.?By studying the mechanism of pain relief and clinical practice means, we can provide theoretical basis and practical guidance for the comprehensive application of photon technology in pain clinical treatment.

Rosacea is a chronic inflammatory skin disease mainly involving the middle face. The exact etiology and pathogenesis have not been fully elucidated, and the clinical treatment is difficult. Although the previous topical drugs and systematic oral drugs have some effects on erythematotelangiectatic rosacea (ETR), they have a long medication cycle, slow onset, and are easy to rebound and relapse. In particular, the subjective symptoms such as flushing and burning are not significantly improved. The compliance and satisfaction of patients are poor and low, and patients are prone to anxiety, depression and other psychological problems. In recent years, with the widespread application of pulsed dye laser, intense pulsed light, Nd:YAG laser, KTP laser, Pro-Yellow laser, photobiomodulation therapy and other photoelectric technologies in clinical treatment of ETR, the options showed a wider range, the overall therapeutic effects are promoted, the satisfactions of both doctors and patients are improved. This article reviews the application of various photoelectric techniques in the treatment of ETR.

In recent years, in order to deal with the excessive CO2 emission caused by fossil fuel combustion and the resulting global warming and energy depletion, CO2 mitigation strategy via carbon fixation and conversion to high-value products by microalgae cultivation system has attracted a great deal of attentions. In particular, the addition of CO2 absorbents has been reported effective to promote microalgal growth and carbon fixation rates. In this study, Chlorella sp. with excellent CO2 tolerance was used as the experimental strain to explore the effects of chemical adsorbent monoethanolamine (MEA) on algal physiological and biochemical characteristics and carbon sequestration efficiency under 20% CO2 concentration with ventilation rate of 0.33. The results showed that adding MEA could alleviate the growth inhibition of microalgae by culture medium acidification caused by high concentration CO2. In addition, MEA with appropriate concentration could also effectively improve the growth, metabolism, CO2 fixation efficiency and photosynthetic activity of Chlorella sp. The biomass concentration, CO2 fixation rate and lipid content of Chlorella sp. all reached maximum value of 3.07 g/L, 0.55 g CO2/(L·d) and 23.5% respectively under 50 mg/L MEA, which were 43.7%, 45.6% and 21.7% respectively higher than those without MEA in control. In summary, 50 mg/L CO2 absorbent monoethanolamine could significantly improve the biomass, lipid accumulation and carbon sequestration efficiency of Chlorella sp. under high CO2 concentration. The above results would provide theoretical basis for CO2 emission reduction and clean energy development by microalgae.

In order to develop a biocontrol agent with high eficiency for rice, an endophytic Streptomyces griseobrunneus Ahn75 with high antagonistic activity aganist rice blast pathogen was used as material, and the effects of different adjuvants, including nutrient, wetting dispersant, stabilizer and UV protective on the germination of Ahn75 spores and the stability of Ahn75 agent were analyzed, then the biocontrol efficencies of excellent adjuvants synergistic with biocontrol strain on rice blast was assessed. The results showed that foliar fertilizer, yeast extract, Tween 80 and dextrin could significantly promote the germination of Streptomyces Ahn75 spores, and dextrin can also protect Ahn75 spores from UV damage at a certain extent, while sodium alginate as a stabilizer showed a less effect on the germination of spores and better stability than other stabilizers. The outdoor pet experiments showed Ahn75 spore suspension combined with each adjuvant can significantly reduce the incidence and disease index of rice leaf blast. Among them, the mixed adjuvants showed best synergistic control effect with a efficacy of 38.06%. Based on the results of indoor and outdoor tests, the best formulation of Ahn75 agent was finally determined as: spore concentration 1×108 CFU/mL, yeast extract (0.05%) as nutrient, Tween 80 (0.05%) as wetting dispersant, dextrin (1.00%) as UV protective, and sodium alginate (0.15%) as stabilizer. This study provides an important basis and support for the field application of biocontrol Streptomyces Ahn75.

NOD-like receptor thermal protein domain associated protein 3 (NLRP3) inflammasome is a multiprotein complex that plays a crucial role in host immune responses to both pathogen infections and sterile injuries. Construction of the NLRP3 inflammasome system in HEK293T cells provides a more convenient and concise method for studying the regulation mechanisms of inflammasome activation. Plasmids NLRP3, apoptosis-associated speck-like protein (ASC), Caspase-1, and pro-1β were transfected into HEK293T cells. Subsequently, the mature IL-1β levels were determined by Western blot and enzyme-linked immunosorbent assay (ELISA) after adding nigericin. Our results showed that the mature IL-1β could be detected in cell lysates and supernatants of cells transfected with intact NLRP3 inflammasome components whereas not in cells transfected with incomplete NLRP3 inflammasome components. Meanwhile, the mature IL-1β increased with the elevated amounts of transfected NLRP3. In this study, NLRP3 inflammasome system was successfully constructed in HEK293T cells, which laid a foundation for future research.

In order to study the effect of alginate lyase (AlgL) on biofilm and bacterial factors RpoS gene of hypervirulent Klebsilla pneumoniae (HvKP), 30 strains of Klebsiella pneumoniae (KP) were isolated from clinical samples. They were identified by automatic rapid microbial mass spectrometry detection system VITEK, were done susceptibility tests for drug resistance analysis, the strains with positive wire drawing test were identified as HvKP. The bacterial biofilm was detected by semi quantitative crystal violet staining. The expression of RpoS gene was detected by polymerase chain reaction (PCR). AlgL can significantly inhibit HvKP biofilm, and the thickness of biofilm is positively correlated with the expression of RpoS gene, indicating that alginate lyase can inhibit the formation of HvKP biofilm. It provides a research basis for AlgL in the clinical treatment of HvKP.

Response of greenhouse gas emissions in soil to climate change is a hot issue of agricultural response to climate change. The contents of nitrate and ammonium nitrogen, greenhouse gas emission flux, and emissions during the growing season in soil, combined with the yield of winter wheat were measured under elevated CO2 concentration (600 μmol/mol) and increased temperature (environment temperature+2℃). The global warming potential (GWP) and greenhouse gas emission intensity (GHGI) of winter wheat during the growing season were calculated. The results showed that the nitrate nitrogen content increased significantly at jointing stage under elevated CO2 concentration and increased temperature. The ammonium nitrogen content of winter wheat increased at the three stages under elevated CO2 concentration and increased temperature. Compared with T0C0, the total CO2 emissions during the growing season increased by 20.73%, 22.18% and 23.28% under T1C0, T0C1 and T1C1, respectively. The total emission of N2O during the growing season decreased by 26.35% and 18.41% under T0C1 and T1C0, respectively. Compared with T0C0, the total uptake of CH4 during the growing season significantly decreased by 93.56% under T0C1 and T1C1. Compared with T0C0, the yield of winter wheat under T1C0 significantly reduced by 19.93%, while it increased by 15.36% and 9.88% under T0C1 and T1C1, respectively. GWP significantly increased under T1C0, T0C1, and T1C1, while T1C0 significantly increased GHGI compared with T0C0. In conclusions, under elevated CO2 concentration and increased temperature in the future, total emissions of CO2 and N2O during growing season has increased to varying degrees. The total emissions of CH4 under increased temperature will be significantly reduced. GWP estimated by monitoring the three greenhouse gases emission in wheat fields under elevated CO2 concentration and increased temperature will provide theoretical support for the formulation of future agricultural emission reduction measures.

Sulfur is an essential macronutrient for plant growth and development, and sulfate transporter (SULTR) plays key roles in sulfate (SO42-) uptake and transport in higher plants. In this study, 10 SULTR genes (CsaSULTR1;1～CsaSULTR4;1) were identified in cucumber. Phylogenetic analysis showed that SULTRs from cucumber and Arabidopsis could be divided into four groups (GROUP 1～4). Most CsaSULTR genes contained 9～12 introns, and closely related members shared similar gene structures. Promoter analysis revealed that?CsaSULTR genes may be involved in responses to hormones and environmental stress.?Expression profiling analysis by RNA-seq indicated that CsaSULTR genes have distinct expression patterns in different tissues, and some of them had significantly changed expression in response to downy mildew infection. Our results provide a better understanding for further functional analysis of CsSULTR?genes in cucumber.

Myopia is the most common visual impairment worldwide, and high myopia is the leading cause of vision loss or even blindness, the high prevalence and blindness of which predispose individuals to a great deal of pain through their whole life.?In this study, we recruited a three-generations Guangxi family with high myopia. The clinical data and genome DNA of 17 people relatives of the family were collected. According to the results of whole exomes sequence, sanger sequence were applied to identifify the causative gene. A novel missense mutation c.790A>T(p.N264Y)in NYX gene was co-segregating with high myopia in this family. This mutation was absent in 100 controls and publically online available SNP databases including the 1000Genomes Project, ESP6500 and ExAc. Previous study reported a c.792C>G(p.N264K)mutation of NYX associated with congenital stationary night blindness. It is indicated that different amino acid changes at position 264 of NYX might lead to distinct phenotypes. Our finding expands the mutation spectrum of NYX and provides a useful information for further elucidation on genotype-phenotype relationships.

Downy mildew is an important systemic disease that reduces the yield and quality of foxtail millet. In the process of foxtail millet resisting to pathogen, resistance genes play a particularly important role. Chitinases (EC,3.2.1.14) are important pathogenesis-related protein families in plant disease resistance. They play a key role in the process of plant defense against pathogen and pest damage. In this study, based on the transcriptome analysis of Sclerospora graminicola-infected resistant and susceptible foxtail millet varieties in the early-stage, it was found that the chitinase family genes were significantly differentially expressed in resistant and susceptible varieties. It is speculated that this family genes might be involved in the regulation of foxtail millet resistance. Therefore, we performed series of bioinformatics assays and experiments on these genes. The locations of 30 selected chitinase family genes in chromosomes were revealed by bioinformatics techniques. TBtools and other software were used to analyze the physical and chemical properties, gene structure, phylogenetic tree construction, protein conserved domain. Transcriptional expressions of chitinase genes were measured by quantitative PCR. The results showed that 30 chitinase genes were distributed on 8 chromosomes except chromosome 6. All chitinase proteins are predicated to be secreted proteins and they all have conserved motifs. Phylogenetic tree analysis revealed that the gene family members could be divided into three subgroups. Combined with the transcriptome data of resistant and susceptible varieties infected by S.graminicola, the expression of up-regulated and down-regulated genes were divided into three expression modes. The results of fluorescence quantitative PCR showed that Seita.1G225300, Seita.4G286000, Seita.5G389900, Seita.7G150600, Seita.7G150700 and Seita.8G076900 were significantly differentially expressed and most likely to be involved in S.graminicola and their expression trends were consistent with transcriptome data. The results are expected to provide a theoretical reference for cloning and molecular breeding of resistance genes in foxtail millet.

In order to identify the high-risk children with early community-acquired pneumonia (CAP) complicated with myocardial injury, the nomogram of CAP complicated with myocardial injury was constructed and evaluated, the nomogram of early CAP complicated with myocardial injury was constructed and evaluated. The medical records of 385 children with CAP were retrospectively analyzed and divided into training set and validation set. The predictors were screened by LASSO regression and Logistic regression, and the nomogram for predicting myocardial injury was developed. The nomogram was evaluated by ROC curve, calibration curve, DCA curve. Age, early chest radiograph characteristics, type of cough, extrapulmonary complication, the highest temperature, lymphocyte percentage, SaO2, CRP and mycoplasma infection were the predictors of early CAP with myocardial injury in children. The AUC value of nomogram in two set were 0.827 and 0.815. The calibration curve was in good agreement with the actual curve. And the DCA curve showed that the use of nomogram can benefit in a wide diagnostic thresholds range. The nomogram with good discrimination and accuracy can identify the risk of myocardial injury in children with early cap and help clinicians to give early warning and timely intervention.