ObjectiveTo investigate the role of ovarian tumor (OTU) domain protein 6B (OTUD6B) in the progression of gastric cancer (GC) and to clarify its related molecular mechanism.MethodsThe differential expression of OTUD6B was analyzed by TIMER 2.0 database. Western blotting and qRT-PCR were used to analyze the expression differences of OTUD6B in gastric cancer tissues and cell lines, and Kaplan-Meier was used to analyze the correlation between OTUD6B expression and prognosis of patients with gastric cancer. The expression of OTUD6B in gastric cancer and adjacent tissues was further detected by immunohistochemistry (IHC). The expression of OTUD6B in HGC-27 and AGS cells was knocked down by lentivirus-mediated gene silencing technique. The effects of OTUD6B on the proliferation of gastric cancer cells were evaluated by CCK-8 and EDU experiments. The effect of OTUD6B on the migration ability of gastric cancer cells was investigated by transwell and scratch tests. The interaction between OTUD6B and the target was verified by co-immunoprecipitation and immunofluorescence techniques.ResultsOTUD6B expression was significantly up-regulated in gastric cancer tissues, and high expression of OTUD6B was associated with poor overall survival. In vitro experiments showed that the proliferation and migration ability of gastric cancer cells were significantly weakened after OTUD6B knockdown. Mechanically, OTUD6B binded and deubiquitinated poly A binding protein cytoplasmic 1 (PABPC1), reducing the level of K48 ubiquitination modification and finally stabilizing PABPC1. The response experiment showed that overexpression of PABPC1 partially reversed the knockdown effect of OTUD6B.ConclusionOTUD6B can stabilize PABPC1 through binding and deubiquitination, and promote the proliferation and migration of gastric cancer cells, suggesting that OTUD6B may be a potential therapeutic target for the progression of gastric cancer.

ObjectiveTo explore the influencing factors of early postpartum pelvic floor dysfunction diseases (PFD) in parturients and establish a column chart model for predicting the risk of postpartum PFD based on Lasso-Logistic regression.MethodsA total of 373 women who delivered successfully in the First Hospital of Xingtai from January 2023 to July 2024 were selected as the study objects and divided into modeling cohort group (n=261) and validation cohort group (n=112). All parturients underwent PFD screening at 6-8 weeks postpartum, and a column chart model was constructed in R4.3.1 based on the influencing factors of postpartum PFD screened by Lasso-Logistic regression analysis. Receiver operating characteristic (ROC) curve, Hosmer-Lemeshow test, and calibration curve were used to evaluate the effectiveness of the model in predicting the risk of postpartum PFD. The clinical application value of model was evaluated by decision curve analysis (DCA).Results72 cases of PFD occurred in the modeling cohort group, 34 cases of PFD occurred in the validation cohort group, and the total incidence of PFD in 373 postpartum women was 28.4%. Compared with the non-PFD group, the average maternal age and average neonatal body mass in the PFD group were higher (P<0.05), and there were statistically significant differences in PFD family history, delivery times, delivery mode, perineal laceration and the second stage of labor between the two groups (P<0.05). Lasso-Logistic regression analysis showed that age of parturients, delivery times, delivery mode, perineal laceration, the second stage of labor, and neonatal body mass were all influencing factors of postpartum PFD (P<0.05). ROC curve analysis showed that the area under the curve (AUC) of the modeling cohort was 0.827 (95% CI: 0.772－0.882), and the AUC of the validation cohort was 0.838 (95% CI: 0.762－0.915). In the Hosmer-Lemeshow test, the modeling cohort showed 2=7.556, P=0.478; the validation cohort showed 2=4.680, P=0.791. DCA showed that the model had high value in clinical application.ConclusionThe age of parturients, delivery times, delivery mode, perineal laceration, the second stage of labor, and neonatal body mass are the influencing factors for postpartum PFD. The column chart model established based on these six factors has better predictive performance and it can help guide clinical prevention of postpartum PFD.

ObjectiveTo evaluate the effect of platelet preparations on the proliferation of gastric cancer cells cultured in vitro, explore the methods that can promote the proliferation of gastric cancer cells, and provide a research basis for cell therapy and the expansion of high-quality gastric cancer cells in the future.MethodsBioinformatics approaches were utilized to assess the association between platelet preparations and gastric cancer cell proliferation; platelet-rich plasma (PRP), platelet-normal plasma (PNP) and platelet-poor plasma (PPP), platelet lysate (PL), platelet gel supernatant (PGS) and fetal bovine serum (FBS) were obtained by different methods. Dilute RPMI 1640 medium with 10% PRP, PNP, PPP, PL, PGS, FBS, and plasma, and co-culture with gastric cancer cells for 48 hours, then cell growth was observed under an inverted microscope. The cell proliferation efficacy in passage gastric cancer cells was evaluated using CCK-8 assays.ResultsSignificant enrichment of intersection genes was observed in signal receptor activator activity and receptor ligand activity categories; Co-culture findings showed optimal growth and typical morphology in FBS and PL groups, with whereas PRP induced partial cells over-differentiation, and average outcomes in PNP, PPP, and PGS groups. CCK-8 assays showed statistical differences in proliferation across groups and times, with FBS having the fastest growth rate, and PL closely matching FBS in OD values upon two-way ANOVA analysis.ConclusionFBS demonstrates optimal performance in six media, with cell proliferation consistently superior across all time points compared to other groups, followed by PL, while PRP and PNP show negligible effects. The study indicates that PL may replace FBS in gastric cancer cell culture, offering experimental support and future perspectives for platelet-based media.

ObjectiveTo screen and clinically validate prognosis-related differentially expressed long non-coding RNAs (LncRNAs) in colon cancer using gene chip technology.MethodsA cohort of 321 colon cancer patients treated between June 2020 and June 2023 was selected for this study. Among them, 34 patients were used for screening differentially expressed LncRNAs, while 287 patients were used for subsequent validation. Of the 34 patients, 9 who experienced poor prognosis one year post-surgery were categorized into Group A, with the remaining 25 patients were assigned to Group B. Gene chip technology was employed to detect differentially expressed LncRNAs in the tumor tissues of Groups A and B. A competing endogenous RNA (ceRNA) network was constructed, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Prognosis-related LncRNAs were screened using the LASSO regression model, and the variables identified were included in a multivariate Cox regression model to analyze factors affecting the prognosis of colon cancer.ResultsA total of 227 differentially expressed LncRNAs were screened between Groups A and B, with 162 upregulated and 65 downregulated LncRNAs. The constructed ceRNA network comprised 181 nodes (93 LncRNA nodes, 29 miRNA nodes, and 59 mRNA nodes) and 603 edges. GO enrichment analysis revealed that the differentially expressed LncRNAs were primarily involved in cellular responses to chemical stress, oxidative stress, and external stimuli, including transcriptional regulatory complexes, RNA polymerase Ⅱ transcription regulatory complexes, and the nuclear outer membrane. These LncRNAs were also involved in DNA-binding transcription factor binding, R-SMAD binding, and RNA polymerase Ⅱ-activated transcription factor binding. KEGG enrichment analysis indicated that the differentially expressed LncRNAs were mainly concentrated in the IL-17 signaling pathway, TNF signaling pathway, oxytocin signaling pathway, and NF-B signaling pathway. LASSO regression identified 11 variables associated with colon cancer prognosis, including LncRNA NEAT1, LncRNA PCAT1, LncRNA CASC11, LncRNA CCAT1, LncRNA PCAT6, LncRNA ZEB1-AS1, LncRNA PSMA3-AS1, LncRNA AC005062.1, LncRNA GAS5, LncRNA MEG3, and LncRNA USP30-AS1. Cox regression analysis revealed that TNM stage, LncRNA CASC11, LncRNA CCAT1, and LncRNA PCAT6 were risk factors for colon cancer prognosis (P<0.05), whereas LncRNA GAS5 and LncRNA MEG3 were protective factors (P<0.05).ConclusionThis study identify 227 differentially expressed LncRNAs in colon cancer patients with varying prognoses. A ceRNA network is successfully constructed, with GO enrichment analysis highlights cellular responses to chemical stress, oxidative stress, and external stimuli, while KEGG enrichment analysis points to significant involvement in the IL-17, TNF, oxytocin, and NF-B signaling pathways. Clinical validation indicates that LncRNA CASC11, LncRNA CCAT1, and LncRNA PCAT6 are risk factors for colon cancer prognosis, while LncRNA GAS5 and LncRNA MEG3 serve as protective factors.

ObjectiveTo explore the clinical relationship between the expression of long intergenic non-coding ribonucleic acid (LINC) 01503 in breast cancer tissue and postoperative recurrence and metastasis.MethodsThree hundred and ninety-two patients with breast cancer in the First Affiliated Hospital of Zhengzhou University from January 2020 to August 2023 were selected for surgical treatment. Samples of surgically resected cancer tissues and adjacent tissues were collected, the relative expression of LINC01503 were detected by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). The relative expressions of LINC01503 in breast cancer tissues with different clinicopathological features were compared. All patients were followed up until March 2024 or postoperative recurrence and metastasis. According to the following up results, they were divided into recurrence and metastasis group and no recurrence and metastasis group. The clinical data and relative expression of LINC01503 in cancer tissues were compared between the 2 groups, the influencing factors of postoperative recurrence and metastasis were analyzed by Cox regression method, and the hazard ratio (HR) and 95% confidence interval (95% CI) were calculated.ResultsThree hundred and eighty-four cases were included after the cases were excluded. The relative expression of LINC01503 in breast cancer tissues was 3.35±0.72, which was higher than that in paracancer tissues (1.00) (P<0.05). The relative expression of LINC01503 in cancer tissues of patients with carcinoma in situ was lower than that of other pathological type (P<0.05). The relative expression of LINC01503 in cancer tissues of patients with triple negative, tumor-node-metastasis (TNM) stage Ⅲ/Ⅳ, low/undifferentiated tumor, vascular cancer thrombus, nerve invasion and highly microsatellite instability were higher than those of other molecular subtypes, TNM stage Ⅰ/Ⅱ, medium/highly differentiated tumor, no vascular cancer thrombus, no nerve invasion and no highly microsatellite instability (P<0.05). The following up period was 5-49 months, median 32 (16, 42) months. The recurrence and metastasis rate was 18.49% (71/384). Molecular subtypes triple negative (HR=2.633, 95% CI: 1.607－4.314), TNM stage Ⅲ/Ⅳ (HR=2.892, 95% CI: 1.560－5.362), low/undifferentiated tumors (HR=3.165, 95% CI: 1.538－6.510), vascular cancer thrombus (HR=2.667, 95% CI: 1.660－4.286), nerve invasion (HR=2.192, 95% CI: 1.505－3.194), highly microsatellite instability (HR=2.002, 95% CI: 1.420－2.821), carbohydrate antigen (CA) 125 (HR=2.121, 95% CI: 1.467－3.066) and relative expression of LINC01503 in cancer tissues (HR=3.083, 95% CI: 1.637－5.807) were independent risk factors for postoperative recurrence and metastasis (P<0.05).ConclusionThe relative expression of LINC01503 in cancer tissues of breast cancer patients is increased, and it is not only related to pathological type, molecular subtype, TNM stage, tumor differentiation degree, vascular cancer thrombus, nerve invasion and highly microsatellite instability, but also a risk factor for postoperative recurrence and metastasis.

ObjectiveTo analyze the differential diagnostic value of multi sequence MRI in high-grade gliomas (HGG) and solitary brain metastases (SBM).MethodsA retrospective analysis was conducted on 60 patients with HGG and SBM treated in our hospital. Among them, 34 patients with HGG were included in the HGG group, and 26 patients with SBM were included in the SBM group. All patients underwent routine MRI scans, including T2WI, T1WI, FLAIR, T1 enhanced scans, and diffusion-weighted imaging (DWI) scans. The signal detection of routine MRI scans and DWI scans, as well as the apparent diffusion coefficient (ADC) and relative apparent diffusion coefficient (rADC), were analyzed, and the ROC curve was used to evaluate their differential diagnostic value for HGG and SBM.ResultsThere was no statistically significant difference in the signal intensity of T1WI and T2WI between the HGG group and the SBM group (P>0.05). The high signal intensity of T1WI enhancement, T2WI-FLAIR plain scan, and T2WI-FLAIR enhancement in the HGG group (73.58% vs 11.54%, 70.59% vs 7.69%, 91.18% vs 15.38%) was higher than that in the SBM group (2: 22.748, 23.735, 34.821, P<0.05). The peritumoral edema areas in both HGG and SBM groups showed high ADC signal and low DWI signal, while the tumor parenchyma areas showed high DWI signal. The ADC signal intensity in the tumor parenchyma area of HGG group was not statistically significant compared to SBM group (P>0.05). There were no statistically significant differences on ADC, rADC of the tumor parenchyma areas and the normal area of the opposite side of the tumor between HGG group and SBM group (P>0.05), while the ADC, rADC in the peritumoral edema area of the HGG group were lower than those in the SBM group (t: 5.332, 6.663, P<0.05). The ROC curve analysis showed that the AUC of ADC, rADC, and the model for differential diagnosis of HGG and SBM in the peritumoral edema area were 0.829, 0.867, and 0.983, respectively. The AUC of the model for differential diagnosis of HGG and SBM was higher than that of the individual differential diagnosis of ADC and rADC in the peritumoral edema area (P<0.05).ConclusionT1WI enhancement, T2WI-FLAIR plain scan, T2WI-FLAIR enhancement, and ADC and rADC in the peritumoral edema area can differentiate HGG and SBM. The model constructed based on this has some assistance differential diagnostic value for HGG and SBM.

ObjectiveTo analyze the acute effect of nitrogen dioxide (NO2) exposure on the incidence of coronary heart disease in residents of Yangzhou city, and to provide theoretical basis for the formulation of comprehensive prevention and control policies of coronary heart disease.MethodsData on the incidence of coronary heart disease in Yangzhou residents were collected from January 1, 2020 to December 31, 2022, and the atmospheric pollutants in Yangzhou during the same period [NO2, fine particulate matter (PM2.5), inhalable particulate matter (PM10), sulfur dioxide (SO2), carbon monoxide (CO) and the daily maximum 8-hour average values of O3 (O3-8) h)] concentration and meteorological data (average daily temperature, relative humidity), the effect of NO2 on the incidence of coronary heart disease was studied by using time series analysis method.ResultsThe average dail concentration of NO2 during the study period was (27.91±14.04) g·m－3. In the single-pollutant model, the number of acute cases of coronary heart disease increased by 4.73% (1.03%, 8.57%) for each IQR increase of NO2 concentration (lag0 d). Two-and three-pollutant models showed that NO2 was still positively associated with acute coronary heart disease after controlling for the confounding effects of other pollutants. Stratification analysis showed that the risk of angina pectoris increased by 4.16% (0.25%, 8.23%) for each IQR increase of NO2 concentration (lag0 d). The risk increased by 5.77% (0.18%, 11.66%) in women; The risk was increased by 5.50% (1.32%, 9.85%) in residents aged ≥65 years. The risk of urban residents was increased by 6.35% (1.36%, 11.58%). The incidence of unmarried/divorced/widowed/living alone residents increased by 11.42% (2.77%, 20.81%); There was no significant effect on the risk of coronary heart disease in different seasons.ConclusionNO2 exposure may increase the probability of acute coronary heart disease, especially angina pectoris. Female, ≥65 years old, urban, unmarried/divorced/widowed/living alone groups are more susceptible to increased NO2 concentration.

ObjectiveTo investigate the effects of growth differentiation factor 11 (GDF11) on the modulation of macrophage polarization through the mTOR pathway in the context of immune regulation during influenza virus infection.MethodsCells were divided into five groups: negative control (NC), Model, GDF11+/+, GDF11－/－, and GDF11－/－+MHY1485 group. Flow cytometry was employed to assess the expression levels of iNOS and CD86 in RAW264.7 cells across the groups. ELISA kits were utilized to measure levels of inflammatory cytokines and immunoglobulins in the culture supernatants of RAW264.7 cells. The expression levels of GDF11, mTOR pathway proteins, inflammatory cytokines, and immunoglobulins were analyzed using real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot, and immunofluorescence assays.ResultsCompared with the NC group, influenza virus RP8 infection promoted M1 polarization of RAW264.7 cells (P<0.05), downregulated GDF11 levels (P<0.05), upregulated inflammatory cytokines (TNF-, IL-6, and IL-1) levels (P<0.05), and downregulated immunoglobulins (IgA, IgG, and IgM) levels (P<0.05), while enhancing phosphorylation of mTOR, AMPK, and AKT proteins (P<0.05). In comparison with the Model group, overexpression of GDF11 significantly inhibited M1 polarization of RAW264.7 cells (P<0.05), decreased levels of inflammatory cytokines (TNF-, IL-6, and IL-1) (P<0.05), increased levels of immunoglobulins (IgA, IgG, and IgM) (P<0.05), and suppressed phosphorylation of mTOR, AMPK, and AKT proteins (P<0.05). Knockout of GDF11 protein significantly promoted M1 polarization in RAW264.7 cells (P<0.05), upregulated the levels of inflammatory factors, downregulated the levels of immunoglobulins (P<0.05), and enhanced the phosphorylation of mTOR, AMPK, and AKT proteins (P<0.05). The mTOR inhibitor MHY1485 effectively suppressed M1 polarization in RAW264.7 cells (P<0.05), reduced levels of inflammatory cytokines (TNF-, IL-6, and IL-1) (P<0.05), increased levels of immunoglobulins (IgA, IgG, and IgM) (P<0.05), and inhibited phosphorylation of mTOR, AMPK, and AKT proteins (P<0.05).ConclusionGDF11 can mitigate M1 polarization of RAW264.7 cells induced by influenza virus infection through the inhibition of the mTOR signaling pathway, thereby contributing to immune regulatory responses during influenza virus infection.

ObjectiveTo analyze the prevalence of cognitive impairment in patients with type 2 diabetes mellitus (T2DM) aged 60 years and above, and to evaluate the effect of length of sleep at night on cognitive impairment.MethodsA multi-stage cluster random sampling method was used to select 1 149 diabetic patients aged 60 years and above from 6 counties and urban areas from May to October 2023. The basic information, lifestyle, disease history and length of sleep at night of the interviewees were obtained through a questionnaire survey, and the existence of cognitive impairment was screened using AD8+CSI-D. Multivariate Logistic regression analysis and restricted cubic spline plot were used to study the effect of length of sleep at night on cognitive impairment.ResultsThe detection rate of cognitive impairment was 43.52%. Multi-factor Logistics regression analysis showed that the risk of cognitive impairment was 1.550 times [95% CI (1.031-2.330)] and 1.555 times [95% CI (1.104-2.189)] for≤5 h and 7.1-8.0 h compared with 6.1-7.0 h for length of sleep at night. Stratified analysis showed that the incidence of cognitive impairment in women with length of sleep at night≤5 h was higher than that in men, and the risk of cognitive impairment in women ≥80 years old with length of sleep at night≤5 h and 7.1-8.0 h was higher than that in 70-79 years old. The results of restricted cubic spline plot showed that the relationship between length of sleep at night and cognitive impairment in elderly diabetic patients was approximately U-shaped.ConclusionLong or short night sleep length is positively associated with cognitive impairment, and there are gender and age differences.

ObjectiveTo investigate the predictive value of serum levels of galectin-1 (Gal-1) and soluble B7-H4 (sB7-H4) in postoperative metastasis of papillary thyroid carcinoma (PTC) patients.MethodsFrom February 2020 to February 2022, 126 PTC patients admitted to our hospital who underwent total thyroidectomy and neck lymph node dissection were collected, and according to postoperative pathological examination for metastasis, patients were divided into metastasis group (37 cases) and non metastasis group (89 cases), 108 volunteers who entered our hospital for physical examination were included as the control group. Enzyme linked immunosorbent assay (ELISA) was applied to detect the levels of Gal-1 and sB7-H4 in serum; Spearman method was applied to analyze the correlation between serum levels of Gal-1 and sB7-H4 and postoperative metastasis in PTC patients; Relevant factors affecting postoperative metastasis in patients with PTC were analyzed by multifactorial Logistic regression; diagnostic value of combined Gal-1 and sB7-H4 assay for postoperative metastasis in PTC patients was analyzed by ROC curve; Clinical utility of serum Gal-1 and sB7-H4 levels and combined assays for predicting the development of metastases after surgery in patients with PTC was analyzed using a DCA.ResultsThe serum levels of Gal-1 and sB7-H4 in PTC patients were obviously higher than those in the control group (P<0.05); The serum levels of Gal-1 and sB7-H4 in PTC patients in postoperative metastasis group were obviously higher than those in the non metastasis group (P<0.05); the serum levels of Gal-1 and sB7-H4 in PTC patients were positively correlated with postoperative metastasis (r: 0.623, 0.682, P<0.05); the levels of Gal-1 and sB7-H4 in serum were risk factors for postoperative metastasis in PTC patients (P<0.05); the area under the curve (AUC) of the combined prediction of Gal-1 and sB7-H4 for postoperative metastasis in PTC patients was 0.869, which was superior to their individual detection (Zcombination-Gal-1=2.012, Zcombination-sB7-H4=2.014, P: 0.044, 0.044), the sensitivity was 89.19%, and the specificity was 69.66%; The results of DCA curves showed that the net benefit of the combined serum Gal-1 and sB7-H4 test in predicting the risk of postoperative metastasis in patients with PTC was superior to that of the two tests alone when the high-risk thresholds ranged from 0.08 to 0.97, and the calibration curve showed good fitting effect, with a slope close to 1.ConclusionThe serum levels of Gal-1 and sB7-H4 in patients are closely related to postoperative metastasis in PTC patients, and the combined examination of the two can assist clinical practice in effectively predicting postoperative metastasis in PTC patients.

ObjectiveTo explore the relationship between interleukin-6 (IL-6) single nucleotide polymorphisms and paroxysmal atrial fibrillation (PAF) in elderly patients with primary hypertension.Methods279 elderly patients with primary hypertension admitted to our hospital from January 2020 to January 2023 were selected as the study subjects. The polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method was used to detect the single nucleotide polymorphisms at the-174G/C and-572C/G sites of the IL-6 gene, and the Hardy Weinberg (H-W) genetic balance test was performed. All patients were followed up until January 2024 and divided into PAF group and non-PAF group according to whether they had concurrent PAF. The general clinical data and IL-6 gene single nucleotide polymorphisms of the two groups were analyzed and compared. Serum IL-6 level was measured by blood samples collected from two groups. The influencing factors of elderly primary hypertension and PAF were analyzed by univariate and multivariate Logistic regression analysis.ResultsThe incidence of PAF in elderly patients with primary hypertension was 19.70% (52/264). The age of patients, the proportion of smoking history, the proportion of hypertension grade Ⅲ, the proportion of GG, GC genotypes, and the frequencies of G alleles at the IL-6-572C/G site in the PAF group were higher than those in the non PAF group (P<0.05). The serum IL-6 level of patients in the PAF group was higher than that in the non PAF group (P<0.05), and the serum IL-6 level of patients with the IL-6-572C/G site GC genotype and GG genotype were higher than those of patients with the IL-6-572C/G site CC genotype (P<0.05). The results of multivariate Logistic analysis showed that age [odds ratio (OR) =1.952, 95% confidence interval (95% CI): 1.281－2.975], smoking history (OR=1.619, 95% CI: 1.145－2.291), hypertension grade Ⅲ (OR=1.752, 95% CI: 1.205－2.548), IL-6-572C/G site GC genotype (OR=1.694, 95% CI: 1.228－2.336), IL-6-572C/G site GG genotype (OR=1.846, 95% CI: 1.176－2.897) were independent risk factors for elderly primary hypertension with PAF (P<0.05).ConclusionSingle nucleotide polymorphisms at the IL-6-572C/G site is associated with elderly primary hypertension with PAF. Age, smoking history, hypertension grade Ⅲ, and IL-6-572C/G site GC and GG genotype are independent risk factors for elderly primary hypertension with PAF.

ObjectiveTo explore the relationship between single nucleotide polymorphism of Toll like receptor 4 (TLR4) gene and vitiligo, and analyze influence factors of vitiligo.MethodsOne hundred and thirty vitiligo patients (observation group) admitted to the hospital from June 2021 to May 2023 were enrolled, and 130 healthy individuals (control group) were enrolled using propensity score matching method from the same period of hospital physical examination centers. Genomic deoxyribonucleic acid (DNA) was extracted from whole blood and single nucleotide polymorphism of TLR4 gene was detected using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Hardy-Weinberg was used to verify compliance with the law of genetic balance. General informations and single nucleotide polymorphisms of TLR4 gene were compared between the two groups. Logistic regression analysis was used to explore the influencing factors of vitiligo.ResultsThe rs2149356, Asp299Gly, and Thr3991le loci of TLR4 genes of two groups all conformed to Hardy Weinberg's law of genetic balance (P>0.05). There were no statistically significant differences in the genotype distribution and allele frequency of the rs2149356 locus of the TLR4 genes between the two groups (P>0.05). The proportion of Asp299Gly and Thr3991le sites of the TLR4 gene in the observation group was 28.46% and 10.00%, respectively, which were higher than the control group's 10.77% and 1.54% (P<0.05). Family history, a preference for stimulating diet, history of skin lesions, Hamilton Anxiety Scale (HAMA) score, Perceived Stress Scale (PSS) score, serum Fe, Asp299Gly site and Thr3991le site of the TLR4 gene were all risk factors for vitiligo (P<0.05), while serum Zn, Cu, and Se were all protective factors for vitiligo (P<0.05).ConclusionThe Asp299Gly and Thr3991le sites of the TLR4 gene are both associated with vitiligo, and family history and a preference for stimulating diet etc. can all affect the risk of vitiligo.

ObjectiveTo analyze the premature mortality rates and changing trends of four major chronic diseases (include cardiovascular and cerebrovascular diseases, cancers, diabetes, and chronic respiratory diseases) in Yixing City, Jiangsu Province from 2015 to 2021, providing evidence and direction for formulating policies to prevent and control major chronic diseases.MethodsThe death surveillance information of registered residents in Yixing City was sourced from the “Wuxi National Health Information Platform”, and the registered population data were obtained from the Wuxi Public Security Bureau. Mortality rates, premature mortality rates, and constituent ratios were calculated. The Joinpoint regression model was used to caculate the average annual percent of change, and the premature death probabilities of these four major chronic diseases in 2030.ResultsThe standardized mortality rate of the four major chronic diseases among registered residents aged 30-69 in Yixing City decreased from 155.56 per 100 000 in 2015 to 140.95 per 100 000 in 2021, and the premature mortality rate of major chronic diseases decreased from 9.35% in 2015 to 8.36% in 2021, both showing a decreasing trend year by year. The proportion of premature deaths from major chronic diseases in the total number of premature deaths increased from 75.49% in 2015 to 77.58% in 2021. It is predicted that the probability of premature death among women will be 3.97% in 2030, and the probabilities of premature death among men and the entire population will be 9.20% and 6.58% respectively.ConclusionAlthough the premature deaths from the four major chronic diseases in Yixing City are at a relatively low level, they are still the main cause of premature deaths among registered residents. High-risk screening and comprehensive prevention and control of cardiovascular and cerebrovascular diseases, and diabetic populations should be focused on, and male populations should be the key targets for intervention.

ObjectiveTo investigate the expressions and clinical significance of GATA binding protein 3 (GATA3) and glucose transporter type-1 (GLUT-1) in breast cancer.MethodsA total of 118 patients with breast cancer who were treated surgically in our hospital from May 2019 to May 2021 were collected, and cancer tissue specimens and paracancerous tissue specimens were retained during the surgery for research. Simultaneously, clinical data such as TNM staging and lymph node metastasis were collected and organized. Immunohistochemistry was applied to detect the expression levels of GATA3 and GLUT-1. The relationship between the expression levels of GATA3 and GLUT-1 and the clinicopathological characteristics in the breast cancer patients was analysed; the correlation was analysed by Spearman rank correlation analysis. The relationship between the expressions of GATA3 and GLUT-1 and the prognosis of patients was analysed by the Kaplan-Meier method. The multifactorial Cox regression analyses were used to analyse the influencing factors of the prognosis of breast cancer patients.ResultsThe positive expression rate of GATA3 was 27.97% in breast cancer tissues, which was lower than that of paracancerous tissues (P<0.05), and the positive expression rate of GLUT-1 was 73.73% in breast cancer tissues, which was higher than that of paracancerous tissues (P<0.05). The proportion of breast cancer tissues with GATA3 positive expression was significantly lower in patients with TNM stage Ⅲ, tumour diameter ≥2 cm and lymph node metastasis than that in breast cancer patients with TNM stageⅠ-Ⅱ, no lymph node metastasis and tumour diameter<2 cm (P<0.05), the proportion of GLUT-1 positive expression was significantly higher than that of breast cancer patients with TNM stage Ⅰ-Ⅱ, no lymph node metastasis and tumour diameter<2cm (P<0.05). GATA3 was negatively correlated with GLUT-1 expression in breast cancer tissues (r=－0.229, P<0.05). The 3-year survival rate of patients with GATA3-positive breast cancer tissues (25/33, 75.76%) was higher than that of patients with GATA3-negative expression (20/85, 23.53%) (2=27.485, P<0.05). The 3-year survival rate of GLUT-1-positive patients (21/87, 24.14%) was lower than that of patients with negative expression of GLUT-1 (24/31, 77.42%)(2=27.503, P<0.05). GATA3, GLUT-1, TNM staging, tumour diameter, and lymph node metastasis were the factors influencing the prognosis of breast cancer (P<0.05).ConclusionThe expressions of GATA3 and GLUT-1 in breast cancer tissues are closely associated with clinicopathological features such as TNM stage and prognosis of patients.

ObjectiveTo investigate the correlation on serum levels of pyruvate dehydrogenase kinase 4 (PDK4), NOD like receptor protein 3 (NLRP3), phosphatase and tensin homolog deleted on chromosome ten (PTEN) with myocardial injury indicators and prognosis in sepsis patients.MethodsFrom September 2021 to September 2023, Collection of 355 patients with first diagnosis of sepsis admitted to our hospital were gathered and separated into sepsis induced myocardial injury (SIMI) group (225 cases) and sepsis alone group (130 cases) based on whether they had myocardial injury, and were divided into the death group (56 patients) and the survival group (169 patients) according to the prognostic survival of the patients after 28 d of treatment. Real-time fluorescence quantitative PCR (qRT-PCR) was applied to detect serum levels of PDK4, NLRP3, and PTEN. Fully automated biochemical analyzer was applied to detect the levels of creatine kinase isoenzyme (CK-MB), cardiac troponin I (cTnI), myoglobin (Mb), and heart type fatty acid binding protein (H-FABP). Pearson correlation coefficient method was applied to analyze the relationship between serum PDK4, NLRP3, PTEN and myocardial injury indicators in SIMI patients. Cox regression was applied to analyze the factors influencing the prognosis of SIMI patients.ResultsThe levels of serum PDK4, NLRP3, PTEN, CK-MB, cTnI, Mb, and H-FABP in the SIMI group were obviously higher than those in the sepsis alone group (P<0.05). The levels of serum PDK4, NLRP3, PTEN in sepsis patients were positively correlated with myocardial injury indicators CK-MB, cTnI, Mb, and H-FABP (P<0.05). The APACHE Ⅱ score, SOFA score, cTnI, and serum PDK4, NLRP3, and PTEN levels in the prognostic death group were higher than those in the survival group (P<0.05). APACHE Ⅱ score, SOFA score, cTnI, PDK4, NLRP3, and PTEN were independent risk factors affecting the prognosis of SIMI patients (P<0.05).ConclusionSerum PDK4, NLRP3, and PTEN are all highly expressed in SIMI patients, and they are independent risk factors affecting the prognosis of SIMI patients.

ObjectiveTo investigate the risk factors for intrauterine infection in gestational diabetes mellitus (GDM) based on the reaction range model (RRM).MethodsA retrospective analysis of clinical data from 573 GDM patients who underwent prenatal check-ups and deliveries at a hospital from January 2019 to January 2024 was conducted. Based on the RRM theory, univariate factors associated with intrauterine infection in GDM patients were collected through literature review and expert consultation. The incidence of intrauterine infection in GDM patients was recorded. GDM patients were divided into an infected group and a non-infected group based on whether they developed intrauterine infection. Differences in general data between the two groups were compared. Binary multi-variate Logistic regression analysis was performed to identify factors influencing intrauterine infection in GDM patients. The theoretical framework of RRM was used to validate the hypotheses regarding intrauterine infection in GDM patients. Hosmer-Lemeshow test and receiver operating characteristic (ROC) curve were used to verify the efficacy of RRM in predicting GDM complicated intrauterine infection.Results8.90% of the 573 GDM patients developed intrauterine infection. Binary multivariate Logistic regression analysis showed that history of GDM (OR=2.242, 95% CI: 1.419－3.542), coexisting polycystic ovary syndrome (PCOS) (OR=2.542, 95% CI: 1.451－4.454), coexisting sexually transmitted diseases (OR=2.894, 95% CI: 1.435－5.835), polyhydramnios (OR=1.713, 95% CI: 1.126－2.606), and glycosylated hemoglobin (HbA1c) (OR=2.869, 95% CI: 1.743－4.722) were risk factors for intrauterine infection in GDM patients (P<0.05). Based on the theoretical framework of RRM, under the influence of various factors, GDM patients entered the reactive stability stage. If the stress factors persist, stability failure will result in intrauterine infection, which was consistent with RRM and basically consistented with the research hypothesis. Hosmer-Lemeshow test was used to evaluate the goodness of fit of the Logistic regression model constructed under the RRM framework (2=0.874, P=0.502). The area under the curve (AUC) for predicting intrauterine infection with GDM was 0.928 (95% CI: 0.887－0.957), sensitivity was 96.15%, and specificity was 87.68%.ConclusionRisk factors for intrauterine infection in GDM include history of GDM, coexisting PCOS, coexisting sexually transmitted diseases, polyhydramnios, and HbA1c based on RRM.

ObjectiveTo investigate the diagnostic value of serum alpha-fetoprotein (AFP) and regenerating islet-derived 4(REG4) levels in endometrial cancer (EC) and their relationship with lymph node metastasis.MethodsA total of 120 newly diagnosed EC patients admitted to our hospital from January 2021 to June 2023 were collected as the EC group. According to the status of lymph node metastasis, they were grouped into lymph node metastasis group (n=68) and non lymph node metastasis group (n=52). Another 80 patients with endometritis during the same period were selected as the benign lesion group, and 65 physically healthy persons were selected as the control group. ELISA was used to detect serum AFP and REG4 levels; Correlation between serum AFP and REG4 and the diagnostic value of both for EC were analyzed. Multivariate Logistic regression was applied to analyze the influencing factors of EC lymph node metastasis.ResultsThe serum AFP and REG4 levels were significantly higher in the benign lesion and EC groups than those in the control group (P<0.05), and serum AFP and REG4 levels were significantly higher in the EC group than those in the benign lesion group (P<0.05). Serum AFP and REG4 levels were positively correlated (r=0.205, P=0.025), and the combination of the two was more effective in diagnosing EC than the diagnosis alone (Zcombination-AFP=4.335, P<0.001, Zcombination-REG4=2.618, P=0.009). The serum AFP and REG4 levels in the lymph node metastasis group were significantly higher than those in the non lymph node metastasis group (P<0.05), and elevated serum AFP and REG4 levels were risk factors for EC lymph node metastasis.ConclusionSerum AFP and REG4 levels were significantly elevated in EC patients, both of which have some diagnostic value for EC and are associated with EC lymph node metastasis.

ObjectiveTo investigate the changes and clinical significance of levels of serum Sal-like protein 4(SALL4) and FMS-like tyrosine kinase 3 ligand (Flt3L) in adult patients with acute lymphoblastic leukemia (ALL).Methods86 adult ALL patients who visited our hospital from October 2021 to October 2023 were selected as the research group. Meantime, 86 healthy volunteers in our hospital were selected as the control group. According to the prognosis of ALL patients, 21 patients were divided into poor prognosis group and 65 patients with good prognosis group. ELISA was applied to measure the expression levels of serum SALL4 and Flt3L. Logistic regression analysis of prognostic factors of ALL patients was done. ROC curves were plotted to analyze the predictive value of serum SALL4 and Flt3L levels for the prognosis of ALL.ResultsCompared with the control group, the serum SALL4 level in the research group was higher (P<0.05), and the Flt3L level was lower (P<0.05). Compared with the good prognosis group, the poor prognosis group had higher serum SALL4 level (P<0.05) and lower Flt3L level (P<0.05). Logistic regression analysis results showed that serum SALL4 and Flt3L were the prognostic factors of ALL patients (P<0.05). The combination of serum SALL4 and Flt3L had the highest AUC (0.971) in predicting the prognosis of ALL patients, which was better than individual prediction (Zcombination-SALL4=2.839, P=0.005; Zcombination-Flt3L=2.201, P=0.028), with a sensitivity of 95.24% and a specificity of 89.23%.ConclusionSerum SALL4 level is elevated and Flt3L level is reduced in ALL patients. The combination of the two is more effective in predicting the prognosis of ALL patients.