ObjectiveTo evaluate the effect of platelet preparations on the proliferation of gastric cancer cells cultured in vitro, explore the methods that can promote the proliferation of gastric cancer cells, and provide a research basis for cell therapy and the expansion of high-quality gastric cancer cells in the future.MethodsBioinformatics approaches were utilized to assess the association between platelet preparations and gastric cancer cell proliferation; platelet-rich plasma (PRP), platelet-normal plasma (PNP) and platelet-poor plasma (PPP), platelet lysate (PL), platelet gel supernatant (PGS) and fetal bovine serum (FBS) were obtained by different methods. Dilute RPMI 1640 medium with 10% PRP, PNP, PPP, PL, PGS, FBS, and plasma, and co-culture with gastric cancer cells for 48 hours, then cell growth was observed under an inverted microscope. The cell proliferation efficacy in passage gastric cancer cells was evaluated using CCK-8 assays.ResultsSignificant enrichment of intersection genes was observed in signal receptor activator activity and receptor ligand activity categories; Co-culture findings showed optimal growth and typical morphology in FBS and PL groups, with whereas PRP induced partial cells over-differentiation, and average outcomes in PNP, PPP, and PGS groups. CCK-8 assays showed statistical differences in proliferation across groups and times, with FBS having the fastest growth rate, and PL closely matching FBS in OD values upon two-way ANOVA analysis.ConclusionFBS demonstrates optimal performance in six media, with cell proliferation consistently superior across all time points compared to other groups, followed by PL, while PRP and PNP show negligible effects. The study indicates that PL may replace FBS in gastric cancer cell culture, offering experimental support and future perspectives for platelet-based media.