At present, improving oleic acid content on the basis of double low quality is a hot spot for the improvement of fatty acid in rapeseed. Traditional quality testing and screening of breeding offspring materials have significant drawbacks such as long cycle, difficult operation, heavy workload and strong professionalism. In this paper, the research progress of the quality characteristics of rapeseed and the application of spectroscopy technology at home and abroad are reviewed. It is demonstrated that spectroscopy technology can be used to detect oleic acid and other fatty acids in vegetative organs of different growth stages of rape, which provides statistics support for rapid evaluation of high oleic rapeseed quality. And it also build a new technical apprroach for screening breeding materials, shortening the breeding cycle, improving detection efficiency, reducing testing costs, and simplifying operational procedures. This has great practical significance and guiding role.

Photodynamic therapy is a treatment mode that uses a specific wavelength of laser to excite photosensitizers to produce reactive oxygen species (ROS) to kill tumor cells. However, the half-life of ROS is very short and can only act to the close site of generation, which obviously limits the efficacy of photodynamic therapy. Organelles are the indispensable parts of cells that can work normally. For this reason, effective targeted delivery of photosensitizers to organelles is an effective strategy to improve the efficacy of photodynamic therapy. In this review, the design principles, targeting strategies, current challenges, and potential future directions of organelle-targeted photosensitizers will be introduced.

Viral pneumonia is one of the common human diseases worldwide, and acute respiratory diseases caused by the outbreak of novel coronavirus in recent years seriously threaten human health. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which broke out in Wuhan, China since December 2019, once again attracts high attention, having been identified by WHO as an Public Health Emergency of International Concern. Seven coronaviruses have been found to infect humans, of which four are more common in the population and less pathogenic. The other three are caused by SARS-CoV (2002), MERS-CoV (2012) and SARS-CoV-2 (2019), symptoms of the diseases are severe. As a special susceptible population, once pregnant women suffer from pregnancy complicated with novel coronavirus pneumonia, it will theoretically affect the mother and fetus. However, at present, domestic and foreign studies on this aspect are limited. Therefore, in order to minimize the adverse consequences on the mother and fetus, it is necessary to strengthen the research on this aspect. This article reviews and analyzes the effects of novel coronavirus on different pregnancy states in first, second and third trimester through the pregnancy outcomes of pregnant women with SARS, MERS, and COVID-19 to provide guidance for the supervision and management of other related novel coronavirus infected pregnant women in the future, and provide a clinical basis for reducing maternal and fetal mortality and doing prevention work .

A hand-held sweep source optical coherence tomography (SSOCT) system was used to image three areas on human hands, including nail, fingertip, and palm. The imaging area size is 3 mm×3 mm. The index compensation algorism was used to reconstruct blood information en face images at different depths. The changes in both the blood vessel perfusion density (PD) and blood vessel diameter(VD) with the imaging depth were then calculated to reveal the characteristics of the microvasculature of human hand. The experimental results showed that in the above three imaging positions, as the imaging depth increases, the blood vessel PD and blood VD both increase, and contains more information about the blood flow. At the same time, the en face image clarity decreases with the increase of the depth. It was also observed that the microvascular at palm are the densest one, and the blood flow information is more abundant, which is more suitable as an imaging site for the diagnosis of microcirculation diseases. The layered projection results showed that when the imaging depth of the area above the nail is relatively shallow (220~660 μm), the blood vessel PD is significantly smaller than the other two imaging sites, indicating that the blood flow information is not abundant in this range. In this work, we measure the geometric characteristics of hand blood flow that varies with depth, and verify the feasibility of applying OCT technology to the diagnosis of subcutaneous microcirculation in the hand. In the early stages of microcirculation disease, microvascular changes have already occurred. Therefore detection of microvascular changes has clinical significance in early diagnosis of the disease.

In this study, a cadmium-resistant strain 134 was isolated and purified from the rice rhizosphere soil polluted by cadmium. The physiological and biochemical characteristics and 16S rDNA were studied. The results showed that strain 134 was a Gram-negative strain and can grow under the concentration of 75 mg/L cadmium. Analysis of 16S rDNA sequence of strain 134 showed it belongs to Aeromonas sp. Amino acids and sugars was chosen as the exogenous chemotacticum, which is the commonly exudates in rice root, and the chemotactic reaction of Aeromonas 134 with the exogenous chemotacticum was investigated. The results showed that Aeromonas 134 has strong positive chemotaxis to histidine, alanine, leucine, glucose and fructose. Capillary tube test suggested that the chemotaxis responses of strain 134 depended on the concentrations of the chemotacticum. In detail, strain 134 showed the strongest chemotactic reaction towards the 80 μmol/L leucine, 40 μmol/L histidine, 60 μmol/L alanine and glucose, 100 μmol/L fructose. On the basis of these, a solution culture experiment was designed to study the effects of exogenous addition of histidine, leucine, glucose and D-fructose on the colonization of strain 134 in the rice rhizosphere. All four exogenous chemotactic agents can promote the colonization of strain 134 in the rice rhizosphere, especially the treatment of glucose and D-fructose, the number of rhizosphere colonizing bacteria was significantly different from the control (P <0.01), and was 3.48 and 3.87 times of the control, respectively.

In this study, we studied the effects of rose extract (RE) on the growth and apoptosis of yeast cells under arsenic stress. Ascorbic acid solution (Vc) was used as the standard antioxidant and 1.0 mmol/L for sodium arsenite solution (As) is the yeast stress concentration. The experiment was divided into control group, 1.5 g/L RE group, 0.1 g/L Vc group, 1.0 mmol/L As group, 1.0 mmol/L As+1.5 g/L RE group and 1.0 mmol/L As+0.1 g/L Vc group. We detected the growth, proliferation and apoptosis of yeast cells by MTT assay, plate method, FDA/PI staining, DAPI staining and Annexin V-FITC/PI apoptosis flow cytometry after yeast cells were cultured for 24 h. The experimental results showed that rose extract effectively relieved the damage of arsenic to yeast cells, and significantly improved the growth rate and survival rate of yeast cells (P<0.01), significantly inhibited the apoptosis of yeast cells (P<0.01). The results confirmed that rose extract had a significant inhibitory effect on the apoptosis of arsenic stressed yeast cells. At 50% DPPH clearance rate, the inhibitory effect of rose extract on the apoptosis of yeast cells under arsenic stress was significantly higher than that of Vc (P<0.05). Therefore, RE as a natural plant extract, due to its green and non-toxic mechanism of action, will have a broader application prospect in combating heavy metal pollution, improving the body’s antioxidant capacity and alleviating oxidative damage.

The objective of the present study was to investigate the effects of astragalus polysacharin (APS) on the expression profile of long non-coding RNA (lncRNA) during brown adipogenesis of mouse C3H10T1/2 cells. 0.4 g/L APS was added to induction medium, and the 1.5 day brown adipogenic induced cells were used for the library construction and sequence, we screened differential mRNAs and differential lncRNAs, and analyzed the differential mRNAs and the target genes predicted by differential lncRNAs cis and co-expression were analyzed functionally. In addition, the expression levels of three random lncRNAs and three mRNAs were detected by fluorescence quantitative PCR to verify the accuracy of the sequencing results. The results indicated that a total of 13 450 lncRNAs and 57 776 mRNAs were obtained by sequencing. The reliability of the sequencing data was good by analyzing the expression amount, length, number of exons and pair repeatability. A total of 153 differentially expressed lncRNAs and 1 227 differentially expressed mRNAs were screened. The results showed that the differential mRNAs GO annotation was enriched in 53 functional categories, and the KEGG analysis was enriched in 343 pathways. The GO annotations of target genes predicted by cis and co-expression of differential lncRNAs were enriched in 34 and 33 functional categories, respectively, and the molecular function items were the same; KEGG analysis showed that many genes were enriched in the signal pathway of fat metabolism and fat differentiation, especially in glucagon and cAMP signal pathway. In conclusion, APS could effectively affect lncRNA expression profile of brown adipogenic induced C3H10T1/2 cell, which will further provide fundamental data for understanding the effect of APS on stem cell differentiation.

In order to establish African swine fever virus droplet digital PCR detection method with high specificity, high sensitivity and good repeatability, three pairs of primers and probes were synthesized based on two conserved sequences of African swine fever virus nucleic acid, VP72 and K205 genes. In this study, the method was optimized and the linearity, specificity, sensitivity and repeatability were compared. It was found that the detection limits of the three pairs of primers and probes were all less than 6 copies / reaction, and there was a good linear relationship between 10~106 copies / reaction. The coefficients of variation within the quantitative range were all less than 15%. Moreover, synthesized three pairs of primers and probes did not cross-react with the two common viruses of pigs. This method is suitable for the detection of African swine fever virus in pork and its products. It is beneficial to detect African swine fever virus in fresh pork and various pork products at various area such as source, food processing, and food sales, and effectively reduce potential risks.

Amplified in breast cancer 1 (AIB1) can promote the tumorigenesis, development and metastasis in breast cancer. However, the association between AIB1 expression and the tumorigenesis and development of ovarian cancer is still not clear. In this study, we extracted the mRNA expression data of ovarian cancer tissues from TCGA database, and analyzed the correlation between aib1 expression and overall survival (OS), post progression survival (PPS) and progression free survival (PFS) in ovarian cancer patients by using online software Kaplan-Meier plotter. Results showed that OS, PPS and PFS were significantly reduced in patients with aib1 high expression in both all patient population and the DDP-treated population. In SKVO3 cell line transfected with AIB1 over expression plasmid and siRNA was found to up-regulate and down-regulate AIB1 expression respectively, and then the sensitivity of cells to DDP (cis-dichlorodiammine platinum, DDP) was detected. Results showed that up-regulation of AIB1 inhibited the sensitivity of SKVO3 cells to DPP, whereas down-regulation of AIB1 promoted the sensitivity of SKVO3 cells to DPP. Further study showed that the high expression of AIB1 up-regulated the expression of anti-apoptotic proteins B-cell lymphoma-2 (BCL2) and B-cell lymphoma/leukemia x long form (BCL2L1) in SKVO3 cells, while the depletion of AIB1 down-regulated the expression of BCL2 and BCL2L1 in ovarian cancer cells. The mRNA expression data of cancer tissues from ovarian cancer patients was extracted from the TCGA database, and the association between aib1 mRNA levels and the mRNA expression of bcl2 and bcl2l1 was analyzed by online software GEPIA. We found that aib1 expression was positively correlated with bcl2 and bcl2l1 expression. These results further suggested that AIB1 might regulate the expression of BCL2 and BCL2L1. Altogether, our results showed that AIB1 could be used as a potential marker for the prognosis of ovarian cancer and a potential molecular target for the treatment of ovarian cancer. Moreover, this study also found that inhibition of AIB1 can enhance the therapeutic effect of DDP in ovarian cancer. This study provided new insights for the pathogenesis of ovarian cancer resistance to DDP, and has certain value for overcoming ovarian cancer chemotherapy resistance.

In this study, the full-length cDNA sequence of HK gene was cloned from Haematococcus pluvialis by the combined homologous cloning and rapid-amplification of cDNA ends (RACE) methods. The physical and chemical properties, structure, and evolution were analyzed by bioinformatics methods. The full-length cDNA of HaeHK was 2 047 bp, it contained an open reading frame of 1 716 bp and encoded a protein of 571 amino acids. The deduced protein had a calculated molecular mass of 60.61 kD with an estimated isoelectric point (pI) of 6.49. Homologous alignment analysis revealed that HaeHK had similarity with other HKs from different organisms, Volvox carteri (50%) and Chlamydomonas reinhardtii (46%). Conserved domains analysis showed that the typical domain of the HKs was highly conserved in the protein sequences of HaeHK, including a remarkable characteristic with a“butterfly” tertiary structure. Phylogenetic analysis of the HKs showed that HaeHK is highly similar to the HKs of high plants and Chlorophyta, which indicated that they were from a common origin. In this study, the gene sequence encoding HK in Haematococcus pluvialis was obtained for the first time, the above results laid the foundation for further study on gene expression and functional identification, which may provide clues for revealing utilization and metabolism molecular mechanism of hexose in Haematococcus pluvialis.

Headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) was used to analyze the volatile substances of dried Capsicum frutescens with 4 different drying methods in this study, including hot air drying, infrared drying, vacuum freeze drying, and sun drying. The results showed that 39 volatile substances were identified, including 9 esters, 10 aldehydes, 10 alcohols, 2 furans, 3 pyrazines, 3 ketones, and 2 organic acids. Esters, aldehydes, and alcohols were the main volatile substances of dried Capsicum frutescens. Principal component analysis (PCA) and heat map clustering results showed that the volatile substances of samples treated by hot air drying had high relevance with the ones with infrared drying compared to vacuum freeze drying and sun drying. Compared with hot air drying and infrared drying, vacuum freeze drying and sun drying exhibited different effects on the volatiles flavor substances of Capsicum frutescens. Some volatile substances had obvious correlation during the drying process, such as esters and alcohols, alcohols and aldehydes, and they could realize the conversion of volatile flavor compounds through chemical reactions. Drying methods had little influence on volatile substances. There were 39, 37, 36, and 34 volatile substances in hot air drying, infrared drying, vacuum freeze drying and sun drying of Capsicum frutescens, respectively. However, drying method had obvious influence on volatile substances content of Capsicum frutescens. Esters, furans, and pyrazines in samples treated by hot air drying and infrared drying had higher content than vacuum freeze drying and sun drying, while the samples treated by vacuum freeze drying and sun drying had higher content in aldehydes than hot air drying and infrared drying ones. Overall, hot air drying and infrared drying had positive effects on enriching the flavor of dried Capsicum frutescens. This study will provide a theoretical basis for the processing and flavor quality control of dried Capsicum frutescens.

In this study, the roots of capscium with good growth were collected from the field where they have been planted for many years. After surface disinfection, an endophytic bacterium PEB40 was isolated from the roots. This strain can produce siderophore to form larger iron phagocytic circles on chrome azurol S (CAS) plates and also has the ability to produce indole-3-acetic acid (IAA) and dissolve organic phosphorus, showing good plant growth potential. The pot experiment showed that the PEB40 strain had significant growth-promoting effect on capscium, and the yield, plant height and spread of capscium after applying the endophytic bacterium PEB40, and it had significant advantages compared with the blank group (P<0.05). The height increased by 18.9%, the spread increased by 20.7%, and the yield increased by 35.3%, indicating that the endophyte PEB40 has a significant growth promoting effect on capscium. According to the 16S rDNA sequence, phylogenetic tree and morphological characteristics, the PEB40 has a high possibility of being a new species of genus Pseudomonas. This study demonstrated that the CAS plate could be used for high throughput screen of bacteria with plant growth promotion potential, and the PEB40 shows a great potential for developing to microbial fertilizer.