As an important research direction in the field of machine learning, deep learning technology has been infiltrated into various fields of scientific research due to its powerful feature of learning ability and excellent portability. In particular, the achievements in the frontier fields of deep learning have played a positive role in the recognition, classification, segmentation and quantification of medical images. Optical coherence tomography (OCT) technology has the characteristics of non-invasive, high resolution, non-invasive acquisition of micron-level high resolution 3D sectional image of biological tissue, and has been widely used in ophthalmology, vascular, dermatology and other biomedical fields. This paper first introduces the basic principles of OCT and deep learning and the advantages of their combination in the field of skin, and then elaborates the application of deep learning in the field of skin OCT medical images, including the analysis and classification of skin cancer, beauty, fingertip image segmentation, and the qualitative and quantitative evaluation of damaged skin. Key words: optical coherence tomography; medical imaging; deep learning; skin disease; convolutional neural network

With the rapid development of the field of bioengineering, cell engineering has been paid more and more attention. Insect cell culture technology, as a new field of bioengineering, has important value in modern biology, among which the genetic engineering research using insect baculovirus as the carrier and insect cells as the receptor has also made breakthrough progress. Insect cells cultured in vitro can express foreign genes efficiently, and insect baculoviruses are harmless to humans and animals, which makes insect cell culture technology being widely used in various fields such as biology, medicine and agriculture. In this paper, the research progress of insect cell culture is reviewed, including the basic components of insect cell culture medium in vitro, the addition of unclear medium, the bioreactor for the large-scale culture of insect cellss and the vector system of insect baculovirus expression and its application. It provides a theoretical basis for the development of serum-free culture medium for insect cells. Key words: insect cells; baculovirus; serum-free culture; mass cell culture

Microsatellites were crucial in the control of SARS-CoV-2 genomic expression, population genetic evolution and host immunity interaction. The NCBI database and microsatellite analysis systems were utilized in this study to screen and test the correlation between the number of different microsatellites and genetic characteristics in the original strain and variants of SARS-CoV-2, as well as to investigate the microsatellite features affecting the genetic diversity of SARS-CoV-2. By bioinfor-matics analysis, the whole-genome sequence database of SARS-CoV-2 was constructed, and information on the genetic charac-teristics, microsatellite numbers and relative position distribution characteristics of microsatellites in the sequence database were collected; correlation analysis between the number of various microsatellites and one-sample Wilcoxon signed-rank non-para-metric tests were performed by biostatistical analysis. The findings revealed that the quantity, proportion, and relative distribut-ing features of various microsatellites in the original strain and SARS-CoV-2 variants (except Lambda and Omicron) were com-parable. The proportion of microsatellites with 2 nucleotide repeats (77%～78%), 3 motif repeats (22%～23%), and 6 bp full-

As a member of the WW domain binding protein family, WBP11 is an important splicing factor involved in the pre-mRNA splicing process. Zebrafish, one of the notable model organisms, contains orthological homologous gene wbp11 in its genome. In order to study the mechanism of WBP11 in the early development in vertebrates, a knockout strain of wbp11 in ze-brafish was constructed using CRISPR/Cas9 technology. Firstly, gene knockout target sites were designed on the second exon

By using phospholipid molecules with hydrophilic and hydrophobic properties as matrix materials, the nanocom-posite liposomes (Lip@Res/VC) containing both fat-soluble resveratrol (Res) and water-soluble vitamin C (VC) have been pre-pared through thin film hydration method and have been further used for cellular antioxidant studies. In the preparation of Lip@ Res/VC, the optimal added amount of Res and VC were determined by evaluating the encapsulation rate of Res and VC, hydra-tion particle size, polydispersal index and zeta potential of nanocomposite liposomes. The results showed that the hydration par-ticle size of Lip@Res/VC was (92.29±5.41) nm and the encapsulation efficiency of Res and VC were (85.13±5.37)% and (64.73±5.51)%, when the added amount of Res was 4.0 mg and VC was 3.5 mg, respectively. The DPPH (2, 2-diphenyl-1-picrylhydrazyl) and ABTS［2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt］free radical scavenging

Angiogenin 4 (ANG4), a novel antimicrobial protein with multiple biological functions, such as antimicrobial, anti-inflammation, angiogenesis and regulating the body’s immune response, plays a key role in innate immunity. The expres-sion of ANG4 in neonatal mice intestine is regulated by some factors including microbial signal and developmental stage. Being an analogue of surface molecules of bacterial cell wall, chitosan oligosaccharide (COS) was selected as a inducer to explore the effects on ANG4 expression and mophology in post weaning mice intestine. qRT-PCR and Western blot analyses showed that the mRNA and protein levels of ANG4 increased suggesting that COS can upregulate the expression of ANG4. The results of HE staining of mouse intestinal tract showed that the height of intestinal villi, the depth of crypt and the value of V/C increased significantly. It indicates that COS can improve the integrity of intestinal mucosae in some extent through either inducing the expression of some intestinal antimicrobial proteins, such as ANG4, or its antimicrobial and immunomodulation activities, and provide the theoretical basis for the applications of COS and ANG4 in controlling the intestinal diseases in piglets. Key words: ANG4; COS; AMPs; qRT-PCR; Western blot

To investigate the effects of quercetin on human chondrosarcoma cell migration, invasion and epithelial intersti-tial transformation (EMT) and the regulatory role of Janus kinase 2/signal transduction and transcription promoter 3 (JAK2/ STAT3) signaling pathway, human chondrosarcoma SW-1353 cells were cultured in vitro and treated with quercetin of differ-ent concentrations for 24 h. The optimal concentration of quercetin (50.0 μmol/L) was selected by cell counting kit (CCK-8) for subsequent experiments. The cells were divided into control group with no intervention, quercetin group with 50.0 μmol/L quercetin, positive control group with 6.0 μmol/L adriamycin, inhibitor group involving 50.0 μmol/L quercetin +50.0 μmol/L JAK2/STAT3 pathway inhibitor AG490, and activator group involving 50.0 μmol/L quercetin +0.5 μmol/L JAK2/STAT3 path-way activator colivelin. After 24 h of intervention, cell morphology, migration, invasion, and expression levels of JAK2/STAT3 pathway and EMT-related proteins were analyzed by inverted microscope, Transwell chamber and Western blot. The experimen-

Chlamydial protease-like activity factor (CPAF) is a Chlamydia trachomatis protease, which activity results in dampened host inflammation signaling, cytoskeletal remodeling, and suppressed neutrophil activation. We used series of bioin-formatics to analyze and predict CPAF protein, physicochemical properties and hydrophobicity, signal peptide, subcellular local-ization, transmembrane protein structure, conserved region and N-glycosylation sites and phosphorylation sites, the secondary and tertiary structure, ligand-binding regions and small molecular compounds, B-/T-cell epitopes and the interacting proteins. CPAF, which consist of 601 amino acids, the relative molecular weight is 67 252.57 Da, the theoretical isoelectric point is 5.68. The CPAF protein contains a signal peptide and has non-transmembrane regions. The conserved domain of CPAF protein is lo-

To explore the prognostic value of cuproptosis-related genes in cervical cancer, the clinical data of cervical cancer patients were downloaded from the TCGA database and randomly divided into training group and validation group. Cupropto-sis-related genes were screened out by univariate Cox, LASSO-Cox and multivariate Cox analysis, and the risk model was con-structed. Overall survival (OS) of the two subgroups and the entire cohort was analyzed by Kaplan-Meier curve, and the prog-nostic value of the model was verified by ROC curve and PCA. Univariate and multivariate analyses were performed to evaluate the independent prognostic value of clinical features and risk scores. The biological functions and pathways between the two subgroups were analyzed by gene ontology (GO) and Kyoto encyclopedia enrichment of genes and genomes (KGEE), and the sensitivities of the two subgroups to drugs were further analyzed. Finally, prognostic models of five cuproptosis-related genes

Based on Nrf2/HO-1/NLRP3 pathway, the protective effects and mechanism of yacon extract on acute liver injury (ALI) induced by carbon tetrachloride (CCl4) in rats were investigated. Forty-eight healthy male SD rats were randomly divided into 6 groups: control group, model group, low, medium and high dose group and silymarin positive group, in which ALI model

To investigate the expression of GSDMB and GSDMD in skin cutaneous melanoma (SKCM) and their correla-tion with prognosis and immune infiltration based on bioinformatics. The expression and variation of GSDMB and GSDMD in SKCM were analyzed by GEPIA2 database and cBioportal database. The prognostic value of GSDMB and GSDMD on SKCM was analyzed by GEPIA2 database. The GSCA database was used to analyze the correlation between the expression level of GSDMB and GSDMD in SKCM and the activity of tumor-related pathways. The TIMER database was used to analyze the rela-tionship between the expression level of GSDMB and GSDMD in SKCM and the infiltration level of immune cells. The results showed that the expression level of GSDMB in SKCM tissue was significantly lower than that in normal tissue, while the ex-pression level of GSDMD was significantly higher than that in normal tissue. In 363 SKCM samples, the total variation rate of GSDMB and GSDMD was 9.64%, with mutations predominating (4.96%). The high expression level of GSDMB and GSDMD was significantly associated with the higher overall survival rate of SKCM patients. GSDMB may activate apoptosis pathway in