By using phospholipid molecules with hydrophilic and hydrophobic properties as matrix materials, the nanocom-posite liposomes (Lip@Res/VC) containing both fat-soluble resveratrol (Res) and water-soluble vitamin C (VC) have been pre-pared through thin film hydration method and have been further used for cellular antioxidant studies. In the preparation of Lip@ Res/VC, the optimal added amount of Res and VC were determined by evaluating the encapsulation rate of Res and VC, hydra-tion particle size, polydispersal index and zeta potential of nanocomposite liposomes. The results showed that the hydration par-ticle size of Lip@Res/VC was (92.29±5.41) nm and the encapsulation efficiency of Res and VC were (85.13±5.37)% and (64.73±5.51)%, when the added amount of Res was 4.0 mg and VC was 3.5 mg, respectively. The DPPH (2, 2-diphenyl-1-picrylhydrazyl) and ABTS［2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt］free radical scavenging