To investigate the effects of quercetin on human chondrosarcoma cell migration, invasion and epithelial intersti-tial transformation (EMT) and the regulatory role of Janus kinase 2/signal transduction and transcription promoter 3 (JAK2/ STAT3) signaling pathway, human chondrosarcoma SW-1353 cells were cultured in vitro and treated with quercetin of differ-ent concentrations for 24 h. The optimal concentration of quercetin (50.0 μmol/L) was selected by cell counting kit (CCK-8) for subsequent experiments. The cells were divided into control group with no intervention, quercetin group with 50.0 μmol/L quercetin, positive control group with 6.0 μmol/L adriamycin, inhibitor group involving 50.0 μmol/L quercetin +50.0 μmol/L JAK2/STAT3 pathway inhibitor AG490, and activator group involving 50.0 μmol/L quercetin +0.5 μmol/L JAK2/STAT3 path-way activator colivelin. After 24 h of intervention, cell morphology, migration, invasion, and expression levels of JAK2/STAT3 pathway and EMT-related proteins were analyzed by inverted microscope, Transwell chamber and Western blot. The experimen-