The demand for portable medical equipment and all-day health monitor continues to increase with the development of the medical industry. Benefiting from the advantages such as flexibility, stretchability, light weight, uniform irradiation and jointing skin surface, organic light-emitting diodes (OLED) are becoming a new type of light source for wearable biomedical devices. In this review, recent advances of OLED in photodynamic therapy, photoplethysmography, oximeter, optogenetics, and photobiomodulation are summarized, and the potential application of OLED in wearable healthcare is highlighted. The future trends of OLED will be focused on the development of long-lived devices with high power density as the light sources for clinical applications.

In order to achieve rapid and in situ detection of active bacteria, inorganic alkaline hydrogen peroxide was used as a “clean” reducing agent to reduce and prepare a kind of rambutan-like Au@Ag alloy nanoparticles, which was used as surface enhanced Raman scattering (SERS) substrate material. The results showed that the constructed rambutan-like Au@Ag alloy nanoparticles had high surface roughness and dense surface nano burrs, and the structure had strong adsorption ability on the surface of bacteria, and did not affect the biological activity of bacteria. The rambutan-like Au@Ag alloy nanoparticles have large number of “nano-tip” and “nano-gap” on the surface of single particles, which can produce strong local surface plasmon resonance effect. The rambutan-like Au@Ag alloy nanoparticles are adsorbed on the bacterial surface, which could effectively increase the SERS “hot spots” on the bacterial surface, and it was easy to operate in the actual detection. Using the above characteristics, under the excitation of 633 nm light, the SERS fingerprint spectrum signals of active bacteria could be effectively obtained. With high sensitivity and efficiency of detection, it could be widely used in the SERS sensing analysis of microbial cells.

Compared to other subtypes of breast cancers, triple negative breast cancers (TNBC) present poorer clinical outcome and prognosis due to their resistance to therapeutic radiation and undefined molecular signature. The current study investigated the mechanism underlying radiation-resistance of TNBC through a newly discovered BCCIP/53BP1 pathway. An automatic high throughput metaphase screening and analyzing system was used to detect the influence of radiation induced chromosomal aberration in BCCIP deficient mouse breast cancer cells by the absence of 53BP1. Immunofluorescent staining and Western blot were then performed to detect the effect of 53BP1 depletion on the repair efficiency of DNA double-strand breaks in BCCIP deficient breast cancer cells. The 53BP1 silencing induced alteration of homologous recombination repair efficiency in BCCIP deficient mouse breast cancer cells were further tested by both DR-GFP report system and sister chromatid exchange assay. Finally, colony formation assay was applied to detect the cell survival rate in 53BP1/BCCIP co-depleted breast cancer cells after radiation exposure. The results showed that the enhanced chromosomal aberration rate in radiation exposed BCCIP deficient mouse breast cancer cells was suppressed by 53BP1 depletion. The level and foci number of DNA double-strand break marker γH2AX in 53BP1/BCCIP co-depleted breast cancer cells were both lower than BCCIP solo-depleted cells. The homologous recombination repair efficiency was significantly recovered in 53BP1 additionally depleted BCCIP null breast cancer cells in response to radiation. The radiation resistant capacity was enhanced by 53BP1 co-deletion in BCCIP deficient cells. Collectively, these results suggested that the DNA double-strand break repair efficiency was reinforced in BCCIP deficient breast cancer cells by removing the inhibition to homologous recombination through 53BP1 simultaneous depletion, and therefore, enhanced cell radiation resistant capacity. These findings will give new insights in the anti-radiation feature of TNBC, and provide potential biomarkers for its radiotherapeutic intervention.

MYO7A is the causative gene of human Usher syndrome (US). Usher syndrome cases caused by MYO7A mutation account for 29% to 55% of Usher syndrome type 1 cases. It has been reported that MYO7A mutations can cause Usher syndrome type 1B (USH1B), including sensorineural hearing impairment and age-dependent retinitis pigmentosa (RP), whereas the molecular mechanism is still unclear. In order to elucidate the molecular mechanism of this gene in inner ear and retina development, we used cloning free CRISPR/Cas9 gene editing technology to establish the zebrafish myo7ab knockout lines. Firstly, two knockout sites of the gene were screened by bioinformatics analysis. Next, the template guide DNA of this gene were amplified by polymerase chain reaction (PCR). Then, the template guide DNA were transcribed into the guide RNA. Finally, the guide RNA and Cas9 protein were co-injected into the 1-cell stage of zebrafish embryos. After the effectiveness analysis, it was proved that the CRISPR/Cas9 is effective in knocking out myo7ab gene. The myo7ab knockout line of zebrafish was established successfully after screening. The establishment of this line laid a foundation for studying the role of myo7ab in the development of the inner ear and retina.

In this study, the MADS-box gene family was identified and analyzed at the genomic level of Ipomoea trifida, a close relative of Ipomoea batatas. The results showed that a total of 38 ItfMADS gene members were identified. According to the analysis of physical and chemical properties, the number of amino acids in each member was very different, and 26 members were alkaline. Subcellular localization indicated that 32 of these members were located in the nucleus. According to the phylogenetic analysis, ItfMADS gene family were divided into type Ⅰ and type Ⅱ. Type Ⅰ consisted of 9 members and type Ⅱ had 29 members. Chromosome localization analysis found that the 38 ItfMADS genes were distributed unevenly on 12 chromosomes. There were 9 members on Chromosome11 (Chr11), while there was only one member on Chr12 and Chr00, respectively. Analysis of conserved domain indicated that all the members contained MADS-box domain, and type Ⅱ members also contained K domain; motif 1 and motif 3 were both important conserved motifs in ItfMADS proteins. Analysis of gene structure showed that the number of introns and exons varied greatly among the 38 ItfMADS genes. Promoter analysis found that cis-acting element of light response were of the most amount. Expression pattern analysis revealed that ItfMADS genes expressed differently in different tissues, and their express responses to different stresses were also different. This study provided a foundation for further functional identification of MADS-box gene of Ipomoea batatas.

Based on network pharmacology, the mechanism of Danggui Sini Decoction on lumbar disc herniation is explored. We systematically searched the Chinese Medicine System Pharmacology Technology Platform (TCMSP) and the Chinese Medicine Potential Target Database (TCM-PTD) to obtain 45 active ingredients and 132 targets of Danggui Sini Decoction, and compared the obtained targets with the Uniprot database. The abbreviation of the loaded gene name was matched. By searching the Human Gene Database (GeneCards) and Mendelian Disease Gene Database (OMIM) disease target database, a total of 379 target of lumbar intervertebral disc herniation was obtained, after importing the table and removing the duplicate target. ClusterProfiler R programming software was used to draw a Venn diagram to obtain 25 targets for the joint action of drugs and diseases. Cytoscape Version 3.6.1 software was used to construct a network of target proteins of the active ingredient of Danggui Sini Decoction-drug disease. The drug-disease co-action target was imported into the functional protein association network platform which is search Tool for Recurring Instances of Neighbouring Genes, STRING, the protein interaction network was obtained, and Cytoscape Version 3.6.1 was used for the protein interaction network topological analysis and annotation analysis, and the work are performed on the graph to obtain core targets AKT1, PTGS2, MAPK1, STAT3, MAPK8, JUN, CASP3, MAPK14, etc. ClusterProfiler R software was used to perform GO function enrichment analysis and KEGG pathway enrichment analysis on the public action targets of disease drugs, and Cytoscape Version 3.6.1 was used to draw the target-pathway network diagram. Paeoniflorin, beta-sitosterol, kaempferol, (+)-catechin, stigmasterol, formononetin and licochalcone were obtained from the effective active ingredients of Danggui Sini Decoction and the key action targets, such as IL6, AKT1, PTGS2, MAPK1, STAT3, MAPK8, JUN, CASP3, MAPK14, IL10, etc. It is found that Danggui Sini Decoction mainly showed MAP kinase activity and protein serine threonine tyrosine kinase activity, heme binding, phosphatase binding, oxidoreductase activity, FMN binding, endopeptidase activity, hormone binding and other functions, involving HIF-1, FoxO, NF-κB, MAPK, signal pathways such as p35 treat lumbar disc herniation. This article reveals the mechanism of action of Danggui Sini Decoction in treating lumbar disc herniation from the perspective of multiple components, multiple targets, and multiple pathways, providing basis and new ideas for new drug development and clinical application.

In response to the requirements of “One Regulatory, Two Reduction and Three Basic”, the use of chemical fertilizers was reduced without reducing production. This paper studies the SPAD value spectral estimation model with different increasing density and reducing fertilizer treatments under the condition of full mechanization and machine-transplanted rice with long seeding age and analyzes the correlation between SPAD value of rice leaves and the first derivative spectral reflectance of the canopy spectrum. The SPAD values estimation model were established based on the sensitive wave lengths and the first derivative spectra parameters. The R2, RMSE and RE were used to evaluate the accuracy of the models. The results found that the 6 treatments reached extremely significant correlations in the 634～652 nm, 655～668 nm, 497～552 nm and 687～711?nm bands, all four bands with a correlation coefficient of more than 0.700. Among the models that use the sensitive wavelength as the independent variable to estimate the SPAD value, the polynomial model of low-density fertilizer reduction had the best estimation effect, with 0.766, 1.185 and 2.152% for the calibration set R2, RMSE and RE, and 0.729, 0.364 and 0.752% for the validation set R2, RMSE and RE, respectively. Among the models that use the spectral index as the independent variable to estimate the SPAD value, the medium-density fertilizer reduction has the best estimation effect and its calibration set R2, RMSE and RE were 0.703, 1.314 and 2.359%, respectively, the validation set R2, RMSE and RE were 0.763, 0.620 and 1.198%, respectively. The study found that under the same density conditions, reducing fertilizer would reduce the SPAD value and weaken growth; the higher the transplanting density, the higher the spectral reflectance; the influence of transplanting density on the spectral reflectance of the rice canopy was greater than the fertilization level. All treatments showed that the difference spectral index (DSI) model effect was the best, indicating that the selection of sensitive wavelengths and spectral parameters is critical to the construction of the model.

MYB-CC gene family is transcription factor containing MYB-DNA binding domain and CC (coiled-coil) domain. The CC domain is a dimer domain that can participate in the interaction with proteins through structure folding. This is unique in plants that contain this domain of MYB-CC transcription factor. In addition to phosphate starvation response1 (PHR1), another 14 proteins in Arabidopsis thaliana also have these two domains. Some MYB-CC genes have shown to regulate phosphate uptake and control phosphate starvation in plants. In order to explore the characteristics and functions of MYB-CC gene family in foxtail millet, the characteristics and expression patterns of MYB-CC gene family in foxtail millet were analyzed by bioinformatics analysis. Finally, the collinearity and selection pressure of MYB-CC gene family in foxtail millet, rice and maize genomes were analyzed. The results showed that 18 genes of MYB-CC family were found on 7 chromosomes; a relatively conservative motif 1 was found in the motif analysis; cis-elements analysis showed that there were 6 MYB-CC genes involved in defense and stress response. The results of gene expression analysis showed that SiMYB-CC6 was specifically expressed in roots and stems. The results can provide reference for the further study of MYB-CC genes family in foxtail millet.

Silicon is an important element in the earth’s crust, and only rice can absorb silicon ion. However, there is little research on the effect of foliar application of silicon fertilizer on rice yield and disease and insect resistance. To study the effect of foliar silicon fertilizer, we analyzed the effects of foliar application of silicon fertilizer on agronomic traits, disease and insect resistance, through field test in rice (Oryza sativa L.). Field test results showed that fertilized rice leaves are darker in color and have stronger stalks, enhanced lodging resistance, and tillering number, the panicle number, the grains number per panicle, thousand-grain weight and yield of rice are increased. Fertilized rice has less infection of striped rice borer and without rice sheath blight compared to control. Leaf inoculation test in vitro further indicated that, foliar application of silicon fertilizer can significantly increase the resistance to sheath blight in rice.

Amphibians are important indicators of ecological environment monitoring. In order to understand the amphibian resource status and threatened factors in the National Nature Reserve of West Dongting Lake, Hunan province, from 2016 to 2018, typical habitats in the reserve were selected and 10 sample lines were set to carry out field survey, and environmental factors, amphibian species, number and threat factors within the sample line were recorded. The results showed that: 14 species of amphibians were recorded, belonging to 1 order, 5 families and 8 genera, accounting for 20.59% of the 68 species of amphibians recorded in Hunan province. Most of the floras are Oriental species, among which there are 9 species in central China and south China, accounting for 64.29%. There were 4 species in the southwest area of south China and central China in the eastern boundary, accounting for 28.57%. Hoplobatrachus chinensis classified as National II Key Wildlife Conservation. After calculation and analysis, the recorded amphibian Shannon-Wiener index and Simpson index were 1.445 4 and 0.649 1 respectively, and the Pielou evenness was 0.149 4. Correlation analysis showed that the species richness and relative abundance of amphibians in different months were not significantly correlated with environmental factors. The amphibian resources in West Dongting Lake National Nature Reserve in Hunan province are abundant, but they are not evenly distributed and a variety of human disturbance factors have potentials impacts on the diversity of amphibians in the region.

In order to explore the anti-obesity mechanism of Pu-erh tea, the effect of MC4R expression mediating the anti-obesity activity of Pu-erh tea extracts in high-fat diet-induced obesity in rats was observed. SD rats were divided into three groups: normal diet control group (ND group), high-fat diet group (HF group) and high-fat diet with Pu-erh tea extracts group (PTE group). Body weight and serum lipids were measured, and fat mass were observed. Expressions of MC4R in epididymal fat tissues of rats were measured by RT-PCR and Western blot. Pu-erh tea extracts prevented the increases of body weight and fat tissues induced by high-fat diet, and it reduced the blood lipids. MC4R mRNA and protein levels were markedly increased in adipose tissue of rats fed with high fat diet for intervention with Pu-erh tea extracts. Pu-erh tea could reduce diet-induced body weights and decrease blood lipids, its mechanism might be related to the regulation of obesity gene MC4R.