The demand for portable medical equipment and all-day health monitor continues to increase with the development of the medical industry. Benefiting from the advantages such as flexibility, stretchability, light weight, uniform irradiation and jointing skin surface, organic light-emitting diodes (OLED) are becoming a new type of light source for wearable biomedical devices. In this review, recent advances of OLED in photodynamic therapy, photoplethysmography, oximeter, optogenetics, and photobiomodulation are summarized, and the potential application of OLED in wearable healthcare is highlighted. The future trends of OLED will be focused on the development of long-lived devices with high power density as the light sources for clinical applications.