Fluorescence liftime imaging (FLIM) of modified hydrophobic bodipy dyes that act as fluorescent molecular rotors shows that the fluorescence lifetime of these probes is a function of the microviscosity of their environment. Incuba

Fluorescence lifetime imaging microscopy (FLIM) has gained popularity as a sensitive technique to monitor the functional/conformational states of reduced nicotinamide adenine dinucleotide (NADH), one of the main compounds of oxida

Streak camera has high temporal resolution and high sensitivity, and is a powerful tool in biomedical study to measure fluorescence lifetime and perform fluorescence lifetime imaging. However, nonuniformity of the gain in the stre

Time-resolved fluorescence anisotropy on the nanosecond time scale is useful for the study of the rapid rotation of macromolecules. A system combining the capabilities of fluorescence spectral imaging with time-resolved fluorescen

We study the uptake and distribution of transferrin (Tf)-conjugated CdSe/CdS/ZnS quantum dots (QDs) in single living HeLa cells with both fluorescence confocal microscopy and three-dimensional (3D) reconstruction technique. By inc

We present a miniature fluorescence sectioning microscope which uses a diode-pumped solid-state (DPSS) laser as the light source and a fast translating diffuser to produce dynamically changing speckle patterns onto the back apertu

Spectral bleedthrough (SBT) ratio is dependent on the level of fluorescence intensity in confocal imaging. Precision Forster resonance energy transfer (FRET) algorithm corrects SBT ratio according to fluorescence intensity and avo

Bim, a proapoptotic member of Bcl-2 family, plays an important role in cell apoptosis. It is generally thought that Bim translocates to mitochondria in response to apoptotic stimuli. We use confocal microscopy to image the tempora

Hepatocellular carcinoma (HCC) may metastasize to many organs. The survival rate is almost zero for metastatic HCC patients. Molecular mechanisms of HCC metastasis need to be understood better and new therapies must be developed.

We report the design and fabrication of a novel and fully integrated polymer-based centrifugal microfluidic disc for rapid automatic allergens detection. All essential steps for a single test including flow valving, sequencing, mi

The approaches to obtaining desired intensity or phase modulation by twisted-nematic liquid crystal display (TN-LCD) have been extensively studied based on the knowledge of the LCD's internal structure parameters. Generally, the T

Conformations of surface atoms in various stages of nanogold-based genechip testing are scanned by the atomic force and scanning tunneling microscope. We intuitively observe the process and differences in probe combination, nuclei

A 37-element solar adaptive optics (AO) system was built and installed at the 26-cm solar fine structure telescope of Yunnan Astronomical Observatory. The AO system is composed of a fine tracking loop with a tip/tilt mirror and a

We propose a novel wavelength-division multiplexed passive optical network (WDM-PON) to simultaneously transmit unicast data and multicast services with upstream data re-modulation in optical network units (ONUs). For each wavelen

We demonstrate a novel 40-Gb/s transmission system over a 10×101-km standard single mode fiber (SSMF) loop. This system features polarization multiplexed quadrature phase shift keying (PolMux-QPSK), lowvoltage modulation of 2-V pe

A novel polarization monitoring scheme for polarization division multiplexed (PDM) systems is proposed. By using a tag light transmitted in different but close wavelength to the data signal as the feedback control, the scheme can

An equal channel spacing Sagnac filter is proposed using a high-birefringent photonic crystal fiber (PCF) with birefringence square to the operation wavelength. A PCF with four smaller central air holes surrounded by larger air ho

An original design of ring-core photonic crystal fiber (RPCF) is proposed. By splicing a section of the homemade RPCF between two segments of single mode fibers (SMFs), a simple modal interferometer is presented and experimentally

Hybrid opto-digital joint transform correlator (HODJTC) is effective for image motion measurement, but it is different from the traditional joint transform correlator because it only has one optical transform and the joint power s

A novel timing jitter reduction system over a round trip 20-km urban fiber link is reported. The phase difference of the ninth harmonic of a high-repetition-rate mode-locked laser between the local and the returned signals is obta

The thermal lens effects in Tm:YAP laser are analyzed by solving the Poisson equation with finite difference method. The thermal focal lengths measured are in the range of 40 ? 90 mm at the pump power of 16?34 W, consistent with t

The applied laser energy absorbed in a local area in laser thermal stress cleaving of brittle materials using a controlled fracture technique produces tensile thermal stress that causes the material to separate along the moving di

We design a laser diode (LD) end-pumped passively mode-locked solid-state laser with a semiconductor saturable absorber mirror. Mode-matching efficiency in the active medium including the thermal effect is analyzed, and the optimu

We report the recent improvement on the petawatt (PW) femtosecond Ti:sapphire chirped pulse amplification (CPA) laser system at Shanghai Institute of Optics and Fine Mechanics (SIOM), Chinese Academy of Sciences. By enlarging the

We present a sparse Bayesian reconstruction method based on multiple types of a priori information for multispectral bioluminescence tomography (BLT). In the Bayesian approach, five kinds of a priori information are incorporated,

We investigate the activation of living monocytic U937 cells induced by interleukin-6 (IL-6) at the single cell level. We employ home-built Raman tweezers to measure the Raman spectra of living U937 cells with and without IL-6 at