Laser & Optoelectronics Progress, Volume. 57, Issue 20, 200001(2020)

Quantitative Phase Microscopy with High Stability

Kai Wen1, Ying Ma1, Meiling Zhang1, Yu Wang1, Chi Fu1, Juanjuan Zheng1, Lixin Liu1, Peng Gao1、*, and Baoli Yao2
Author Affiliations
  • 1School of Physics and Optoelectronic Engineering, Xidian University, Xi'an, Shaanxi 710071, China
  • 2State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, Shaanxi 710119, China
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    Figures & Tables(19)
    Measurement of 3D profile and refractive index via phase imaging
    Schematic of DHM optical path. (a) Imaging principle of DHM; (b) interference pattern of object light and reference light
    Reconstruction results of DHM. (a) Reconstructed amplitude; (b) reconstructed phase
    Common-path interference microscopy. (a) Fizeau interference microscopy; (b) Mirau interference microscopy
    Off-axis point-diffraction digital holographic microscopy[57]. G, grating; IP, image plane; L1-L2, lenses (f1 and f2 are focal distances of L1 and L2, respectively); SF, spatial filter (expanded in the inset); VPS, virtual source point
    Imaging results of off-axis point-diffraction DHM[57]. (a) Quantitative phase image of whole blood smear; (b) temporal fluctuations of the spatial standard deviation of the field of view without sample and arbitrary single point in the field of view. σ is the temporal standard deviation for these two signals
    Optical path for phase-shifting point-diffraction DHM with common-path and in-line configuration based on diffraction grating[61]. G1 and G2, Ronchi phase grating; L1--L6, lens; MO1 and MO2, objective; P, polarizer combination; P1--P3, polarizer combination; PH, pinhole filter
    Holographic patterns and reconstructed result obtained with coaxial phase shifting point-diffraction DHM[61]. (a)--(d) Holographic patterns with phase shifts of 0, π/2, π, and 3π/2; (e) reconstructed phase
    SLM based common-path phase-shifting digital holographic microscopy[63]. (a) On-axis illumination; (b) off-axis illumination
    Experimental setup of SMIM[64]
    Diagram of single-beam in-line digital holographic microscopy[90]
    Experimental hologram and reconstruction results[90]. (a) Reconstructed result of conventional back propagation algorithm (with twin image); (b) reconstructed result after 500 iterations by CS algorithm (without twin image); (c)(d) enlarged areas of the dotted box in Figs. 12 (a) and (b), respectively
    Quantitative phase contrast microscopy with parallel light illumination[92]
    Diagram of phase contrast microscopy optical path based on SLM[82]. (a) Surrounding grating; (b) center grating; (c)--(e) phase shift interferograms with phase shifts between the diffracted and undiffracted parts of 0, π/2, and π, respectively
    Quantitative phase contrast microscopy based on polarization modulation[96]
    Measurement results of airflow based on quantitative phase contrast microscopy with polarization modulation[96]. (a) Four-step phase-shift interference patterns of airflow; (b) reconstructed phase distribution of airflow
    Setup of UO-QPM and reconstruction results[101]. (a) Schematic of UO-QPM system; (b) reconstruction result of cos7 cells
    Diagram of optical path of improved Zernike phase contrast imaging[84]
    Imaging results of microlens array based on quantitative phase contrast microscopy with multi-point off-axis illumination[84]. (a)--(c) Interference patterns with phase shifts of 0, -π/2, and π/2, respectively; (d) reconstructed phase distribution of microlens arrays
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    Kai Wen, Ying Ma, Meiling Zhang, Yu Wang, Chi Fu, Juanjuan Zheng, Lixin Liu, Peng Gao, Baoli Yao. Quantitative Phase Microscopy with High Stability[J]. Laser & Optoelectronics Progress, 2020, 57(20): 200001

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    Paper Information

    Category: Reviews

    Received: Mar. 19, 2020

    Accepted: Apr. 20, 2020

    Published Online: Oct. 13, 2020

    The Author Email: Peng Gao (peng.gao@xidian.edu.cn)

    DOI:10.3788/LOP57.200001

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