Fast Detection of Foodborne Pathogenic Bacteria by Laser-Induced Fluorescence Spectrometry

Yu LIU; Zeng-wei LI; Zhi-peng DENG; Qing-xian ZHANG; Li-kou ZOU

doi:10.3964/j.issn.1000-0593(2021)09-2817-06

Spectroscopy and Spectral Analysis, Volume. 41, Issue 9, 2817(2021)

Fast Detection of Foodborne Pathogenic Bacteria by Laser-Induced Fluorescence Spectrometry

Yu LIU^1、1;, Zeng-wei LI^2、2;, Zhi-peng DENG^1、1;, Qing-xian ZHANG^{1、1; *;}, and Li-kou ZOU^{2、2; *;}

Author Affiliations

¹1. Key Laboratory of Geosciences and Nuclear Technology, Chengdu University of Technology, Chengdu 610059, China

²2. College of Resources, Sichuan Agricultural University, Chengdu 611130, China

show less

Abstract Get PDF(in Chinese)

Figures & Tables(12)

Fig. 1. Structure of portable 405 nm laser-induced fluorescence detector

Download full size

View in Article

Fig. 2. The instrument and operation flow chart

Download full size

View in Article

Fig. 3. P=10~100 mW, c=1.5OD, the fluorescence spectra of E.faecalis (a); and Relationship between Intensity and Power (b)

Download full size

View in Article

Fig. 4. Fluorescence spectra of bacteria samples

Download full size

View in Article

Fig. 5. Division of characteristic areas

Download full size

View in Article

Fig. 6. The standard cluster point of training set (a), sample cluster points of test set (b)

Download full size

View in Article

Table 1. Parameters of the laser
View table
View in Article
Table 1. Parameters of the laser
技术指标参数
波长/nm 405
电压/mV 2 000
功率/mW 5~100
分辨时间/ns 500

Table 2. Parameters of the spectrometer
View table
View in Article
Table 2. Parameters of the spectrometer
技术指标参数
探测器 HamantsS10420
结构背薄式, 2D
像素值 14 μm²
探测器有效面积/mm 28.672水平×0.896垂直
积分时间 8 ms~10 s
信噪比 450:1

Table 3. P=50 mW, λ=405 nm, peak wavelength and relative intensity of bacteria samples

View table

View in Article

Table 3. P=50 mW, λ=405 nm, peak wavelength and relative intensity of bacteria samples

No.	致病菌种类	峰值数目	峰值波长 /nm	峰值归一化强度
1	E.faecalis (粪肠球菌)	2	467 528	0.833 7 1
2	S.Typhimurium (鼠伤寒沙门氏菌)	2	467 514	0.962 5 1
3	P.aeruginosa (铜绿假单胞菌)	4	467 516 634 703	0.348 6 0.319 7 1 0.304 8
4	NS(生理盐水)	1	467	1

Table 4. List of intrinsic fluorophores with relatively high fluorescence quantum efficiency along with their absorption and emission maxima^[12]

View table

View in Article

Table 4. List of intrinsic fluorophores with relatively high fluorescence quantum efficiency along with their absorption and emission maxima^[12]

Chromophore (发光团)	Absorption /nm	Fluorescence /nm
Tryptophan (色氨酸)	220, 280, 288	320~350
Tyrosin(络氨酸)	220, 275	305
NADH, NADPH (还原性辅酶, 还原酶)	260, 340	470
Flavins(黄素)	260, 370, 450	530
ATP(三磷酸酰胺)	260	300~500
Coproporphyrin (粪卟啉)	398, 497, 531, 565, 620	622
Protoporphyrin (原卟啉)	406, 505, 540, 575, 630	633

Table 5. Characteristic areas and values
View table
View in Article
Table 5. Characteristic areas and values
No. 特征区域特征量标准化
1 459, 473 a1 1
2 482, 495 a2 a2/a1
3 495, 508 a3 a3/a1
4 508, 521 a4 a4/a1
5 521, 534 a5 a5/a1
6 534, 547 a6 a6/a1
7 547, 560 a7 a7/a1
8 560, 573 a8 a8/a1
9 573, 586 a9 a9/a1

Table 6. Correct recognition rate of E.faecalis and S. Typhimurium by DC
View table
View in Article
Table 6. Correct recognition rate of E.faecalis and S. Typhimurium by DC
No. 细菌
种类测试集
数据正确识
别数据错误识
别数据未识别
数据识别率
/%
1 粪肠球菌 18 18 0 0 100
2 鼠伤寒沙门氏菌 18 18 0 0 100

Tools

Get Citation

Copy Citation Text

Yu LIU, Zeng-wei LI, Zhi-peng DENG, Qing-xian ZHANG, Li-kou ZOU. Fast Detection of Foodborne Pathogenic Bacteria by Laser-Induced Fluorescence Spectrometry[J]. Spectroscopy and Spectral Analysis, 2021, 41(9): 2817

Download Citation

EndNote(RIS)BibTex Plain Text

Set citation alerts for article

Save article for my favorites

Paper Information

Category: Research Articles

Received: Jan. 2, 2021

Accepted: --

Published Online: Oct. 29, 2021

The Author Email:

DOI:10.3964/j.issn.1000-0593(2021)09-2817-06

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology

Table 1. Parameters of the laser

Table 1. Parameters of the laser

Table 2. Parameters of the spectrometer

Table 2. Parameters of the spectrometer

Table 3. P=50 mW, λ=405 nm, peak wavelength and relative intensity of bacteria samples

Table 3. P=50 mW, λ=405 nm, peak wavelength and relative intensity of bacteria samples

Table 4. List of intrinsic fluorophores with relatively high fluorescence quantum efficiency along with their absorption and emission maxima[12]

Table 4. List of intrinsic fluorophores with relatively high fluorescence quantum efficiency along with their absorption and emission maxima[12]

Table 5. Characteristic areas and values

Table 5. Characteristic areas and values

Table 6. Correct recognition rate of E.faecalis and S. Typhimurium by DC

Table 6. Correct recognition rate of E.faecalis and S. Typhimurium by DC

Table 4. List of intrinsic fluorophores with relatively high fluorescence quantum efficiency along with their absorption and emission maxima^[12]

Table 4. List of intrinsic fluorophores with relatively high fluorescence quantum efficiency along with their absorption and emission maxima^[12]