Fig. 1. Flow chart of Voronoï analysis for SMLM image

Fig. 2. Distribution analysis of CD59 by Voronoï diagram. (a) SMLM image of erythrocyte CD59 and Voronoï tessellation of the mass center of CD59 clusters; (b) corresponding conventional fluorescence image of CD59; (c) γ distribution of Voronoï polygon area of CD59 clusters; (d) nearest distance analysis of CD59 clusters

Fig. 3. Simulation and Voronoï analysis of the points with different distributions. (a) Ideal points with random distribution; (b) points adjusted to disks with a certain radius (set to 15 nm), defining as exclusive distribution; (c) points with triangular lattice distribution; (d)(e) effects of point density on the loss function of γ distribution fitting and x_peak value derived from Voronoï diagram for random and exclusive distributions

Fig. 4. Voronoï analysis and nearest distance analysis for SMLM images of different membrane skeletal proteins in erythrocytes. (a) Erythrocyte actin; (b) N terminus of β-spectrin; (c) TMOD; (d) ankyrin; (e) schematic of ultrastructural model of erythrocyte membrane skeleton

Fig. 5. Effects of deletion alone on Voronoï analysis results. (a) Voronoï tessellation of simulated points with lattice distribution after deletion; (b)(c) effect of deletion ratio on loss function and x_peak; (d)(e) γ distribution of Voronoï diagram and nearest distance analysis of simulated points with 0.55 deletion ratio

Fig. 6. Effects of disturbance and deletion on Voronoï analysis results. (a) Voronoï tessellation of simulated points with lattice distribution after disturbance and deletion; (b)(c) effect of disturbance ratio on loss function and x_peak; (d) nearest distance analysis of simulated points with 0.15 disturbance ratio