Laser & Optoelectronics Progress, Volume. 56, Issue 7, 070005(2019)

Research Progress on Label-Free Microscopic Imaging Technology

Jia Zhang, Liang Hong, Sheng Ren, Feifan Zhou, Rui Hu, Junle Qu, and Liwei Liu*
Author Affiliations
  • Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong 518060, China
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    Figures & Tables(9)
    Schematic of OCT system
    Angio-OCT images of human retina. (a) Large field of view (12 mm×16 mm) for Angio-OCT image of human retina; (b) optic nerve papilla image; (c) fovea image; (d) temporal region image (field of view is 2.0 mm×2.4 mm)[9]
    MIP of palm skin.(a)MIP of the epidermal-dermal junction; (b) MIP of the smaller vessels and captllary loops at the epidermal-dermal junction; (c) MIP of the bigger vesels under the horizontal plexus[21]
    Summary of morphological features revealed by THG microscopy[29]
    Adenovirus clusters images of immersed microsphere lens. (a) High magnification SEM image of adenovirus clusters; (b) low magnification SMON image of adenovirus clusters (100 μm BaTiO3 microsphere coupled with a 503 objective lens, NA=0.75); (c) higher magnification SEM image of adenovirus clusters; (d) higher magnification SMON image of adenovirus clusters (100 μm BaTiO3 microsphere coupled with an 803 objective lens, NA=0.9)[37]
    Doppler OCT imaging of hemodynamics inside heart tube of a live mouse embryo
    THG/SHG endomicroscopy results of ex-vivo human brain tissues. (a) Neuronal somata tissue; (b) gray matter neuropil tissue; (c) low-cellularity white matter area; (d) high-grade glioma[55]
    CARS microscope tracking lipid droplet formation experiment. (a) Progress of differentiation of mesenchymal stem cells into adipose; (b) volume change of the lipid droplet depicted by the Imaris software (Bitplane); (c1) comparison between the surface increase of the main acceptor lipid droplet and the net surface provided by the fusing lipid droplet; (c2) comparison between the volume increase of the main acceptor lipid droplet and the net volume of all fusing lipid droplets[63]
    Multimodal imaging of living mouse ears. (a) image of PAI; (b) image of OCT;(c) MAP image of FLM; (d) fused image of PAI, OCT, and FLM; (e) OCT/PAI tomography image corresponding to 1 in Fig. 9 (a); (f) OCT/PAI tomography image corresponding to 2 in Fig. 9(a)[66]
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    Jia Zhang, Liang Hong, Sheng Ren, Feifan Zhou, Rui Hu, Junle Qu, Liwei Liu. Research Progress on Label-Free Microscopic Imaging Technology[J]. Laser & Optoelectronics Progress, 2019, 56(7): 070005

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    Paper Information

    Category: Reviews

    Received: Aug. 6, 2018

    Accepted: Nov. 20, 2018

    Published Online: Jul. 30, 2019

    The Author Email: Liwei Liu (liulw@szu.edu.cn)

    DOI:10.3788/LOP56.070005

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