Chinese Journal of Lasers, Volume. 49, Issue 15, 1507202(2022)

Research Progress in Optical Interference Microscopy Toward Three-Dimensional Imaging of Biological Samples

Tianlong Man, Yuhong Wan*, Mengjing Jian, Wenxue Zhang, Minghua Zhang, Teng Ma, and Qin Zhang
Author Affiliations
  • Faculty of Science, Beijing University of Technology, Beijing 100124, China
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    Figures & Tables(23)
    Several main mechanisms for object- and reference-beam interference under different illumination conditions. (a) Coherent illumination; (b) partially coherent illumination; (c) spatial incoherent illumination, where CPM is the computer-generated phase mask for beam dividing
    Optical path structure of conventional off-axis digital holographic microscopy system[2]
    Optical path of common-path off-axis digital holographic microscopy system[23]
    Reconstructed red blood cells (RBCs) phase images[51]. (a) Reconstructed phase image of stomatocyte; (b) reconstructed phase image of discocyte; (c) reconstructed phase image of stomatocyte after processing by watershed segmentation algorithm; (d) reconstructed phase image of discocyte after processing by watershed segmentation algorithm; (e) phase image of individual RBC obtained after segmentation; (f)(g)(h) images obtained by further segmentation of phase image of individual RBC with labeled watershed segmentation algorithm
    Monitoring of neural cell dynamics using digital holographic phase contrast microscopy[52]. (a) Quantitative phase contrast image of a patched mouse cortical neuron; (b) local excitation on the neuron triggered a strong transient decrease of phase signal
    In-focused reconstructed images at different depth-planes[55]. (a) 0.960 mm; (b) 0.995 mm; (c) 3.268 mm; (d) 12.010 mm; (e) 2.090 mm; (f) 8.620 mm; (g) 1.050 mm; (h) 3.039mm; (i) 4.440 mm
    Large field-of-view and high resolution microscopy imaging of biological samples based on lens-free coaxial digital holographic system[68]. (a) Full field-of-view lens-free amplitude image of invasive ductal carcinoma of human breast; (b) zoomed-in regions outlined by the yellow squares in Fig. (a); (c) traditional microscopy imaging; (d) holograms
    Scheme of optical path and basic principle of parallel phase shifting digital holography[77]. (a) Optical path; (b) details of spatial-multiplexed parallel polarizing component that mounted in front of image sensor; (c) in the recorded hologram, different phase shifting values are assigned to different pixels
    Coaxial digital holographic three-dimensional tomographic imaging realized based on compressive sensing algorithm[82]. (a) Recorded hologram; (b)(c) images of 3D object at two different axial planes; (d) compressive sensing reconstructed images at different reconstruction distances
    Schematic of point diffraction interferometer based interference microscopic imaging system[22]. (a) Schematic of optical path; (b) quantitative phase contrast imaging results of red blood cells
    Quantitative phase-contrast images[95]. (a) Quantitative phase-contrast image of stripy sarcomere captured by digital holographic microscopy system with coherent light; (b) quantitative phase-contrast image of stripy sarcomere captured by digital holographic microscopy system with low coherence ultrashort pulse laser; (c) two-dimensional height profiles along the red and blue lines in (a) and (b)
    Schematic of frequency domain OCT system[96]
    Imaging results of retina using frequency domain OCT system[97]
    Example of a bronchial cross section from a chronic obstructive pulmonary disease sample[99] (scale bars: 0.5 mm). (a) Histology image; (b) attenuation coefficient shown on a logarithmic scale; (c) OAxU, displayed from 0 to 1; (d) relative optic axis orientation, displayed from 0°-180°
    Optical path of FINCH three-dimensional fluorescence microscopy system and three-dimensional reconstructed images of pollen. (a) Optical path[13]; (b) three-dimensional reconstructed images[120]
    Three-dimensional tomographic imaging using compressive FINCH[129]
    Imaging results of computational adaptive optics three-dimensional fluorescence microscopy[134]. (a)(d) Wide-field fluorescence microscopic imaging results in a system with optical aberration; (b)(e) FINCH reconstructed images obtained by using conventional diffraction propagation algorithm; (c)(f) computational adaptive optic (AO) corrected images; (g) reconstructed images of microspheres without (upper row ) and with (lower row) computational AO correction, scale bar represents 880 nm
    Super resolution fluorescence self-interference holographic imaging based on birefringence device[14]. (a)(c) Wide-field microscopic images of Golgi apparatus; (b)(d) super resolved holographic reconstructed images of Golgi apparatus at same numerical aperture
    Wide-field/confocal dual-mode fluorescence self-interference super resolution imaging[130]. (i) Wide-field and self-interference FINCH super resolved images of fluorescence beads; (ii) microtubule obtained by confocal microscopy and confocal self-interference holographic super resolved microscopy CINCH
    Schematic of SELFI fluorescence self-interference three-dimensional super resolution microscopy[10]
    SELFI super resolved imaging of thick biological sample[10]
    Three-dimension localization based on self-interference digital holography[141]. (a) Scheme of imaging principle;(b) hologram of a single fluorescent microsphere; (c) localization results plotted in three dimensions
    • Table 1. Comparisons of different optical interference three-dimensional microscopic imaging techniques

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      Table 1. Comparisons of different optical interference three-dimensional microscopic imaging techniques

      TechniqueIlluminationAdvantageLimitationSpatial imaging resolutionPotential applications
      Digital holographySpatially and temporally coherentNon-scanning three-dimensional (3D) imaging, quantitative phase contrast imagingCoherent noise, phase wrapping, hard to access the structures inside the samplesDiffraction limitedMorphology of biological samples
      Partially coherent digital holographyPartially coherentQuantitative phase contrast, low speckle noiseNecessary sample/system scanning, phase wrappingDiffraction limited3D phase contrast imaging of biological samples
      Optical coherence tomographyPartially coherent3D tomography, high axial resolution2D/3D scanning is necessaryLateral: diffraction limited, axial: on the micrometer orderOphthalmology imaging, endoscopic tomography
      Incoherent digital holographySpatially incoherent,temporally coherentNon-scanning 3D imaging for fluorescent/spatial incoherent sampleLimited signal-to-noise ratioLateral: ~100 nm, axial: less than diffraction limited3D super resolved fluorescence microscopy
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    Tianlong Man, Yuhong Wan, Mengjing Jian, Wenxue Zhang, Minghua Zhang, Teng Ma, Qin Zhang. Research Progress in Optical Interference Microscopy Toward Three-Dimensional Imaging of Biological Samples[J]. Chinese Journal of Lasers, 2022, 49(15): 1507202

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    Paper Information

    Category: Biomedical Optical Imaging

    Received: Jan. 11, 2022

    Accepted: Feb. 28, 2022

    Published Online: Jul. 29, 2022

    The Author Email: Wan Yuhong (yhongw@bjut.edu.cn)

    DOI:10.3788/CJL202249.1507202

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