Fig. 1. Schematics of fabrication of large-area micro-nano topography structures and regulation of cell behavior

Fig. 2. Prepared micro-nano topography structures. (a1)-(a3) Groove structure; (b1)-(b3) broken line structure; (c1)-(c3) wave 1 structure; (d1)-(d3) wave 2 structure; (e1)-(e3) wave 3 structure

Fig. 3. AFM images and contact angles of topography structures. 3D morphology of (a) film, (b)groove, (c) broken line, (d) wave 1, (e) wave 2, and (f) wave 3 structures with corresponding cross-sectional profile images shown below; (g) height statistic of topography structure; (h) contact angles of untreated and treated film substrates

Fig. 4. Migration trajectories of cells cultured on different structures for 20 h with directions of cell migration shown by arrows. (a) Film; (b) groove; (c) broken line; (d) wave 1; (e) wave 2; (f) wave 3

Fig. 5. Analysis of cell morphology on topography structures. (a) Confocal fluorescence images of cells on different topography structures; (b) statistic of cell perimeter; (c) statistic of cell spreading area; (d) statistic of cell roundness; (e) schematics of migration mechanism of cells on different topography structures

Fig. 6. Morphological characterization of annulus and hexagonal structures. (a1)-(a3) SEM images of annulus structures; (b1)-(b3) SEM images of hexagonal structures; (c) AFM image of annulus structure; (d) AFM image of hexagonal structure

Fig. 7. Confocal fluorescence images of cells on patterned topography structures with cell outline shown by dashed line. (a1) Fluorescence image of cells on annulus structure; (a2) magnified fluorescence image at dot box in Fig. 7(a1); (a3) magnified fluorescence image at dotted wireframe in Fig. 7(a1); (b1) fluorescence image of cells on hexagonal structure; (b2) local magnification of fluorescence image in line region; (b3) local magnification of fluorescence image in corner region; (c1) fluorescence image of cell on film substrate; (c2) magnified fluorescence image at dot box in Fig. 7(c1); (c3) magnified fluorescence image at dotted wireframe in Fig. 7(c1)

Fig. 8. Cell viability on topography structures