Chinese Journal of Lasers, Volume. 51, Issue 9, 0907018(2024)
Label‐free Quantitative Microscopic Imaging Technique Based on Hyperspectral Interferometric Reconstruction
Fig. 1. (a) Schematic diagram of hyperspectral interference signals and (b) reconstruction algorithm example
Fig. 2. (a) Electromechanical and (b) optical structure of hyperspectral interferometric imaging system
Fig. 3. (a) Control and (b) software design of the hyperspectral interferometric imaging system
Fig. 5. Hyperspectral interferometric reconstruction results. (a) Scanning electron microscopic imaging of the phantom; (b) hyperspectral interference imaging of the phantom; (c) three-dimensional reconstruction of the phantom; (d) bright-field microscopic imaging of a single HeLa cell; (e) hyperspectral interferometric imaging of the same cell; (f) pseudo three-dimensional reconstruction of the cell
Fig. 6. Comparison of the dry mass and distribution of two different cells. (a) Total dry mass changes across the entire cell cycle of the two cells; (b) comparison of the nucleo-cytoplasmic ratio of the two different cells; (c) lateral imaging resolution test
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Rongxin Fu, Ai Xin, Shuhao Zhang, Jie Fei, Shan Qin, Hanqi Hu, Tianqi Zhou, Shuailong Zhang, Hang Li. Label‐free Quantitative Microscopic Imaging Technique Based on Hyperspectral Interferometric Reconstruction[J]. Chinese Journal of Lasers, 2024, 51(9): 0907018
Category: biomedical photonics and laser medicine
Received: Dec. 1, 2023
Accepted: Feb. 5, 2024
Published Online: Apr. 26, 2024
The Author Email: Fu Rongxin (furongxin@bit.edu.cn)
CSTR:32183.14.CJL231463