Chinese Journal of Lasers, Volume. 51, Issue 9, 0907018(2024)

Label‐free Quantitative Microscopic Imaging Technique Based on Hyperspectral Interferometric Reconstruction

Rongxin Fu1、*, Ai Xin1, Shuhao Zhang2, Jie Fei3, Shan Qin1, Hanqi Hu1, Tianqi Zhou1, Shuailong Zhang4, and Hang Li1
Author Affiliations
  • 1School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China
  • 2School of Automation, Beijing Institute of Technology, Beijing 100081, China
  • 3CSSC Systems Engineering Research Institute, Beijing 100094, China
  • 4School of Integrated Circuits and Electronics, Beijing Institute of Technology, Beijing 100081, China
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    Figures & Tables(6)
    (a) Schematic diagram of hyperspectral interference signals and (b) reconstruction algorithm example
    (a) Electromechanical and (b) optical structure of hyperspectral interferometric imaging system
    (a) Control and (b) software design of the hyperspectral interferometric imaging system
    (a) Structure and (b) performance of the miniature live cell incubator
    Hyperspectral interferometric reconstruction results. (a) Scanning electron microscopic imaging of the phantom; (b) hyperspectral interference imaging of the phantom; (c) three-dimensional reconstruction of the phantom; (d) bright-field microscopic imaging of a single HeLa cell; (e) hyperspectral interferometric imaging of the same cell; (f) pseudo three-dimensional reconstruction of the cell
    Comparison of the dry mass and distribution of two different cells. (a) Total dry mass changes across the entire cell cycle of the two cells; (b) comparison of the nucleo-cytoplasmic ratio of the two different cells; (c) lateral imaging resolution test
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    Rongxin Fu, Ai Xin, Shuhao Zhang, Jie Fei, Shan Qin, Hanqi Hu, Tianqi Zhou, Shuailong Zhang, Hang Li. Label‐free Quantitative Microscopic Imaging Technique Based on Hyperspectral Interferometric Reconstruction[J]. Chinese Journal of Lasers, 2024, 51(9): 0907018

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    Paper Information

    Category: biomedical photonics and laser medicine

    Received: Dec. 1, 2023

    Accepted: Feb. 5, 2024

    Published Online: Apr. 26, 2024

    The Author Email: Fu Rongxin (furongxin@bit.edu.cn)

    DOI:10.3788/CJL231463

    CSTR:32183.14.CJL231463

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