Chinese Journal of Lasers, Volume. 50, Issue 9, 0907401(2023)

Rapid Analysis Method for Liquid‐Liquid Phase Separation Condensate Morphology Based on Sparse Data

Xiyu Jia, Lingran Gong, Yuanyuan Xu, and Ying Ji*
Author Affiliations
  • School of Physics and Electronic Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China
  • show less
    References(27)

    [1] Brangwynne C P. Phase transitions and size scaling of membrane-less organelles[J]. The Journal of Cell Biology, 203, 875-881(2013).

    [2] Hyman A A, Weber C A, Jülicher F. Liquid-liquid phase separation in biology[J]. Annual Review of Cell and Developmental Biology, 30, 39-58(2014).

    [3] Molliex A, Temirov J, Lee J H et al. Phase separation by low complexity domains promotes stress granule assembly and drives pathological fibrillization[J]. Cell, 163, 123-133(2015).

    [4] Banani S F, Lee H O, Hyman A A et al. Biomolecular condensates: organizers of cellular biochemistry[J]. Nature Reviews Molecular Cell Biology, 18, 285-298(2017).

    [5] Alberti S, Gladfelter A, Mittag T. Considerations and challenges in studying liquid-liquid phase separation and biomolecular condensates[J]. Cell, 176, 419-434(2019).

    [6] Wu R B, Li P L. Liquid-liquid phase separation and biomolecular condensates[J]. Chinese Science Bulletin, 64, 2285-2291(2019).

    [7] Li P L, Banjade S, Cheng H C et al. Phase transitions in the assembly of multivalent signalling proteins[J]. Nature, 483, 336-340(2012).

    [8] Mehta S, Zhang J. Liquid-liquid phase separation drives cellular function and dysfunction in cancer[J]. Nature Reviews Cancer, 22, 239-252(2022).

    [9] Banani S F, Rice A M, Peeples W B et al. Compositional control of phase-separated cellular bodies[J]. Cell, 166, 651-663(2016).

    [10] Langdon E M, Gladfelter A S. A new lens for RNA localization: liquid-liquid phase separation[J]. Annual Review of Microbiology, 72, 255-271(2018).

    [11] Schmidt H B, Görlich D. Transport selectivity of nuclear pores, phase separation, and membraneless organelles[J]. Trends in Biochemical Sciences, 41, 46-61(2016).

    [12] Wegmann S, Eftekharzadeh B, Tepper K et al. Tau protein liquid-liquid phase separation can initiate tau aggregation[J]. The EMBO Journal, 37, e98049(2018).

    [13] Boulay G, Sandoval G J, Riggi N et al. Cancer-specific retargeting of BAF complexes by a prion-like domain[J]. Cell, 171, 163-178.e19(2017).

    [14] Luby-Phelps K. The physical chemistry of cytoplasm and its influence on cell function: an update[J]. Molecular Biology of the Cell, 24, 2593-2596(2013).

    [15] Guan Y J, Ma X C. Application of optical microscopic imaging technology in liquid-liquid separation research[J]. Journal of Sun Yat-Sen University (Medical Sciences), 43, 504-510(2022).

    [16] Setru S U, Gouveia B, Alfaro-Aco R et al. A hydrodynamic instability drives protein droplet formation on microtubules to nucleate branches[J]. Nature Physics, 17, 493-498(2021).

    [17] Bai C, Peng T, Min J W et al. Dual-wavelength in-line digital holography with untrained deep neural networks[J]. Photonics Research, 9, 2501-2510(2021).

    [18] Park J, Brady D J, Zheng G A et al. Review of bio-optical imaging systems with a high space-bandwidth product[J]. Advanced Photonics, 3, 044001(2021).

    [19] Yang Z W, Zhang L, Lü N et al. Progress of three-dimensional, label-free quantitative imaging of refractive index in biological samples[J]. Chinese Journal of Lasers, 49, 0507201(2022).

    [20] Zeng M L, Shang Y, Araki Y et al. Phase transition in postsynaptic densities underlies formation of synaptic complexes and synaptic plasticity[J]. Cell, 166, 1163-1175(2016).

    [21] Ji Y, Huang J B, Li X et al. Fast three-dimensional morphological reconstruction algorithm of phase objects based on orthogonal sampling[J]. Acta Optica Sinica, 41, 2312006(2021).

    [22] Li T T, Feng D W, Li Q et al. Quantitative detection method of fluorescence immunochromatography based on microscopic digital image[J]. Chinese Journal of Lasers, 47, 1107001(2020).

    [23] He W L, Dou J T, Yang Z M et al. Sequential shift absolute phase aberration calibration in digital holographic phase imaging based on Chebyshev polynomials fitting[J]. IEEE Photonics Journal, 12, 6900111(2019).

    [24] Tang M, Liu C, Wang X P. Autofocusing and image fusion for multi-focus plankton imaging by digital holographic microscopy[J]. Applied Optics, 59, 333-345(2020).

    [25] Zheng N N, Huang G. Small animal living three-dimensional optical imaging system and its quantitative analysis of breast cancer[J]. Acta Laser Biology Sinica, 31, 215-223(2022).

    [26] Gong L R, Jia X Y, Xu Y Y et al. Three-dimensional morphological reconstruction of nucleated cell based on orthogonal dual-wavelength measurement[J]. Chinese Journal of Lasers, 49, 2007206(2022).

    [27] Majeed H, Sridharan S, Mir M et al. Quantitative phase imaging for medical diagnosis[J]. Journal of Biophotonics, 10, 177-205(2017).

    Tools

    Get Citation

    Copy Citation Text

    Xiyu Jia, Lingran Gong, Yuanyuan Xu, Ying Ji. Rapid Analysis Method for Liquid‐Liquid Phase Separation Condensate Morphology Based on Sparse Data[J]. Chinese Journal of Lasers, 2023, 50(9): 0907401

    Download Citation

    EndNote(RIS)BibTexPlain Text
    Save article for my favorites
    Paper Information

    Category: Bio-Optical Sensing and Manipulation

    Received: Jan. 9, 2023

    Accepted: Feb. 21, 2023

    Published Online: Apr. 24, 2023

    The Author Email: Ji Ying (jy@ujs.edu.cn)

    DOI:10.3788/CJL230447

    Topics