Chinese Journal of Lasers, Volume. 50, Issue 9, 0907401(2023)

Rapid Analysis Method for Liquid‐Liquid Phase Separation Condensate Morphology Based on Sparse Data

Xiyu Jia, Lingran Gong, Yuanyuan Xu, and Ying Ji*
Author Affiliations
  • School of Physics and Electronic Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China
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    Figures & Tables(9)
    Optical path diagram of non-orthogonal interference phase imaging device (M: total reflective mirror; BS: beam splitter prism; L: lens; D: diaphragm; MO: microscope objective; POL: electric rotating polarizer; P: parallel component; S: vertical component)
    Two-medium concentric ellipsoid model. (a) Three-dimensional space diagram of the model ; (b) phase shift distribution of the model in X direction
    Concentric ellipsoid model edge detection results. (a) Canny edge operator detection map of the model in YOZ plane; (b) LOG edge operator detection diagram of the model in YOZ plane; (c) projection boundary of the model in YOZ plane; (d) projection boundary of the model in the other plane
    Principle diagram of direct linear transformation algorithm
    Projection boundary and enlarged local boundary of the model in YOZ plane
    Reconstruction results of two-medium concentric ellipsoid model. (a) Three-dimensional distribution of model reconstruction result; (b) boundary point distribution of axial section of external ellipsoid; (c) boundary point distribution of axial section of inner sphere
    Phase diagrams and inversion morphology of polystyrene micro-sphere. (a)-(b) Phase diagrams of two incidental beam with an angle of 60° in incidental direction; (c) reconstructed three-dimensional morphological distribution; (d) axial section boundary point distribution and fitting evaluation
    • Table 1. Coordinates of non-orthogonal direction control points of two-medium concentric ellipsoid model

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      Table 1. Coordinates of non-orthogonal direction control points of two-medium concentric ellipsoid model

      Serial numberX direction projectionI direction projectionThree dimensional coordinates
      y /pixelz /pixeli* /pixelz /pixelX /μmY /μmZ /μm
      11421421201422.828422
      21421161201162.82842-2
      31161421071422.8284-22
      41161161071162.8284-2-2
      5142142151142-2.828422
      6142116151116-2.82842-2
    • Table 2. Reconstruction data of two-medium concentric ellipsoid model

      View table

      Table 2. Reconstruction data of two-medium concentric ellipsoid model

      ItemTheoretical value /µmCalculate value /µm

      Relative error /

      %

      Time /s
      Axial distribution of ellipsoidal outer layerMajor axis3634.942.940.0689
      Minor axis2827.970.11
      Axial distribution of spherical innerDiameter87.417.37
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    Xiyu Jia, Lingran Gong, Yuanyuan Xu, Ying Ji. Rapid Analysis Method for Liquid‐Liquid Phase Separation Condensate Morphology Based on Sparse Data[J]. Chinese Journal of Lasers, 2023, 50(9): 0907401

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    Paper Information

    Category: Bio-Optical Sensing and Manipulation

    Received: Jan. 9, 2023

    Accepted: Feb. 21, 2023

    Published Online: Apr. 24, 2023

    The Author Email: Ji Ying (jy@ujs.edu.cn)

    DOI:10.3788/CJL230447

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