Spectroscopy and Spectral Analysis, Volume. 42, Issue 10, 3203(2022)

Analysis of Composition Differences of Fresh Radix Rehmanniae, Crude Radix Rehmanniae and Processed Radix Rehmanniae Using Infrared Spectroscopy

Jia-yi TIAN*, Fang MA, and Ling-yu HAN*;
Author Affiliations
  • School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China
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    Figures & Tables(9)
    ATR-FTIR spectra of the fresh RR (a), crude RR (b) and processed RR (c) extract samples
    ATR-FTIR spectra of standard for saccharide compoundsd: Stachyose; e: Galactose; f: Manninotriose; g: Fructose; h: Anhydrous glucose; i: Acteoside; j: Melibiose; k: Mannose
    ATR-SDIR spectra of the fresh RR (a″), crude RR (b″) and processed RR (c″) extract samples
    ATR-SDIR spectra of standard for saccharide compoundsd″: Stachyose; e″: Galactose; f″: Manninotriose; g″: Fructose; h″: Glucose; i″: Acteoside; j″: Melibiose; k″: Mannose
    Ion Chromatography of saccharides mixed standard
    Ion Chromatography of the fresh RR (a), crude RR (b) and processed RR (c) extracts
    Contents of 8 kinds of monosaccharides and oligosaccharides in fresh RR, crude RR and processed RR extracts (n=5)the difference in fresh RR with crude RR and processed RR: pppp<0.01: ##
    • Table 1. Gradient elution procedures

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      Table 1. Gradient elution procedures

      Time/min015253030.13535.14040.147
      A/%9797004040009797
      B/%33100100505010010033
      C/%000010100000
      Curve5555555555
    • Table 2. Linear regression equations of 8 kinds of monosaccharides and oligosaccharides

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      Table 2. Linear regression equations of 8 kinds of monosaccharides and oligosaccharides

      样品回归方程R2线性范围/
      (μg·mL-1)
      葡萄糖y=2.933 7x+0.180 80.999 20.2~20
      蜜二糖y=1.983 1x+0.100 90.999 20.2~20
      果糖y=0.887 0x+0.782 80.999 02~200
      半乳糖y=2.933 7x+0.180 80.999 20.2~20
      甘露糖y=2.786 9x+0.217 80.999 30.2~20
      甘露三糖y=2.162 2x+0.072 40.999 50.2~20
      水苏糖y=1.303 1x+0.140 50.999 12~200
      毛蕊花糖苷y=0.999 0x+2.094 40.999 02~200
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    Jia-yi TIAN, Fang MA, Ling-yu HAN. Analysis of Composition Differences of Fresh Radix Rehmanniae, Crude Radix Rehmanniae and Processed Radix Rehmanniae Using Infrared Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2022, 42(10): 3203

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    Paper Information

    Category: Research Articles

    Received: Jul. 23, 2021

    Accepted: Feb. 7, 2022

    Published Online: Nov. 23, 2022

    The Author Email: Jia-yi TIAN (tianjy1198@163.com)

    DOI:10.3964/j.issn.1000-0593(2022)10-3203-07

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