Visualization Study of Cellular Inflammation Models <i>in vitro</i> Based on Stimulated Raman Imaging (Invited)

Xianzhen Zhou; Mengjie Wang; Jiaojiao Zhang; Lin Shi; Nan Wang; Dongjie Zhang; Qi Zeng; Xueli Chen

doi:10.3788/LOP251161

Laser & Optoelectronics Progress, Volume. 62, Issue 18, 1817019(2025)

Visualization Study of Cellular Inflammation Models in vitro Based on Stimulated Raman Imaging (Invited)

Xianzhen Zhou^1,2, Mengjie Wang^1,2, Jiaojiao Zhang^1,2, Lin Shi^1,2,3, Nan Wang^1,2,3, Dongjie Zhang^1,2,3, Qi Zeng^1,2,3、**, and Xueli Chen^1,2,3、*

Author Affiliations

¹Advanced Diagnostic-Therapy Technology and Equipment Key Laboratory of Higher Education Institutions in Shaanxi Province, School of Life Science and;Technology, Xidian University, Xi'an 710126, Shaanxi , China

²Xi'an Key Laboratory of Intelligent Sensing and Regulation of Trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an 710126, Shaanxi , China

³State Key Laboratory of Electromechanical Integrated Manufacturing of High-Performance Electronic Equipment, Xidian University, Xi'an 710071, Shaanxi , China

show less

Abstract Get PDF(in Chinese)

References(30)

[1] Patil K R, Mahajan U B, Unger B S et al. Animal models of inflammation for screening of anti-inflammatory drugs: implications for the discovery and development of phytopharmaceuticals[J]. International Journal of Molecular Sciences, 20, 4367(2019).

[2] Zhang S, Fang T H, Yue S H. Research advances in disease biomolecular markers revealed by stimulated Raman scattering microscopy[J]. Chinese Journal of Lasers, 51, 1507202(2024).

[3] Lee H J, Cheng J X. Imaging chemistry inside living cells by stimulated Raman scattering microscopy[J]. Methods, 128, 119-128(2017).

[4] Genchi L, Laptenok S P, Liberale C. Background signals in stimulated Raman scattering microscopy and current solutions to avoid them[J]. Advances in Physics X, 8, 2176258(2023).

[5] Shi L Y, Fung A A, Zhou A. Advances in stimulated Raman scattering imaging for tissues and animals[J]. Quantitative Imaging in Medicine and Surgery, 11, 1078-1101(2021).

[6] Zhang C. Coherent Raman scattering microscopy of lipid droplets in cells and tissues[J]. Journal of Raman Spectroscopy, 54, 988-1000(2023).

[7] Rensonnet A, Tipping W J, Malherbe C et al. Spectral fingerprinting of cellular lipid droplets using stimulated Raman scattering microscopy and chemometric analysis[J]. Analyst, 149, 553-562(2024).

[8] Ranjan R, Sirleto L. Stimulated Raman scattering microscopy: a review[J]. Photonics, 11, 489(2024).

[9] Du J J, Su Y P, Qian C X et al. Raman-guided subcellular pharmaco-metabolomics for metastatic melanoma cells[J]. Nature Communications, 11, 4830(2020).

[10] Hedegaard M, Matthäus C, Hassing S et al. Spectral unmixing and clustering algorithms for assessment of single cells by Raman microscopic imaging[J]. Theoretical Chemistry Accounts, 130, 1249-1260(2011).

[11] Fu D, Xie X S. Reliable cell segmentation based on spectral phasor analysis of hyperspectral stimulated Raman scattering imaging data[J]. Analytical Chemistry, 86, 4115-4119(2014).

[12] Braddick H J, Tipping W J, Wilson L T et al. Determination of intracellular esterase activity using ratiometric Raman sensing and spectral phasor analysis[J]. Analytical Chemistry, 95, 5369-5376(2023).

[13] Tipping W J, Wilson L T, An C et al. Stimulated Raman scattering microscopy with spectral phasor analysis: applications in assessing drug-cell interactions[J]. Chemical Science, 13, 3468-3476(2022).

[14] Tipping W J, Wilson L T, Tomkinson N C O et al. Label-free screening of drug-induced liver injury using stimulated Raman scattering microscopy and spectral phasor analysis[J]. Analytical Chemistry, 96, 10639-10647(2024).

[15] Hislop E W, Tipping W J, Faulds K et al. Label-free cytometric evaluation of mitosis via stimulated Raman scattering microscopy and spectral phasor analysis[J]. Analytical Chemistry, 95, 7244-7253(2023).

[16] Fu D, Yu Y, Folick A et al. In vivo metabolic fingerprinting of neutral lipids with hyperspectral stimulated Raman scattering microscopy[J]. Journal of the American Chemical Society, 136, 8820-8828(2014).

[17] Zhao L K, Zheng S Y, Yang W J et al. An image thresholding approach based on Gaussian mixture model[J]. Pattern Analysis and Applications, 22, 75-88(2019).

[18] Mafi M, Martin H, Cabrerizo M et al. A comprehensive survey on impulse and Gaussian denoising filters for digital images[J]. Signal Processing, 157, 236-260(2019).

[19] Ganesan P, Sajiv G. A comprehensive study of edge detection for image processing applications[C], 1-6(2017).

[20] Seal A, Das A, Sen P. Watershed: an image segmentation approach[J]. International Journal of Computer Science and Information Technologies, 6, 2295-2297(2015).

[21] Chen X, Wu Z Q, He Y X et al. Accurate and rapid detection of peritoneal metastasis from gastric cancer by AI-assisted stimulated Raman molecular cytology[J]. Advanced Science, 10, e2300961(2023).

[22] Zheng X, Zhang W, Hu X T. Different concentrations of lipopolysaccharide regulate barrier function through the PI3K/Akt signalling pathway in human pulmonary microvascular endothelial cells[J]. Scientific Reports, 8, 9963(2018).

[23] Chen W W, Chien C H, Wang C L et al. Automated quantitative analysis of lipid accumulation and hydrolysis in living macrophages with label-free imaging[J]. Analytical and Bioanalytical Chemistry, 405, 8549-8559(2013).

[24] Zeng Q, Peng Y Y, Zhou X Z et al. Label-free Raman imaging for screening of anti-inflammatory function food[J]. Food Chemistry: X, 22, 101297(2024).

[25] Cai X X, Bao L, Dai X Q et al. Quercetin protects RAW264.7 macrophages from glucosamine-induced apoptosis and lipid accumulation via the endoplasmic reticulum stress pathway[J]. Molecular Medicine Reports, 12, 7545-7553(2015).

[26] Gater D L, Widatalla N, Islam K et al. Quantification of sterol-specific response in human macrophages using automated imaged-based analysis[J]. Lipids in Health and Disease, 16, 242(2017).

[27] Pi J, Li T, Liu J X et al. Detection of lipopolysaccharide induced inflammatory responses in RAW264.7 macrophages using atomic force microscope[J]. Micron, 65, 1-9(2014).

[28] Caicedo J C, Cooper S, Heigwer F et al. Data-analysis strategies for image-based cell profiling[J]. Nature Methods, 14, 849-863(2017).

[29] Mahapatra S, Shivpuje S B, Campbell H C et al. Label-free quantification of apoptosis and necrosis using stimulated Raman scattering microscopy[EB/OL]. https://www.biorxiv.org/content/10.1101/2025.03.01.641010v1

[30] Tague N, Lin H N, Lugagne J B et al. Longitudinal single-cell imaging of engineered strains with stimulated Raman scattering to characterize heterogeneity in fatty acid production[J]. Advanced Science, 10, e2206519(2023).

Tools

Get Citation

Copy Citation Text

Xianzhen Zhou, Mengjie Wang, Jiaojiao Zhang, Lin Shi, Nan Wang, Dongjie Zhang, Qi Zeng, Xueli Chen. Visualization Study of Cellular Inflammation Models in vitro Based on Stimulated Raman Imaging (Invited)[J]. Laser & Optoelectronics Progress, 2025, 62(18): 1817019

Download Citation

EndNote(RIS)BibTex Plain Text

Set citation alerts for article

Save article for my favorites

Paper Information

Category: Medical Optics and Biotechnology

Received: May. 7, 2025

Accepted: Jun. 17, 2025

Published Online: Sep. 11, 2025

The Author Email: Qi Zeng (qzeng@xidian.edu.cn), Xueli Chen (xlchen@xidian.edu.cn)

DOI:10.3788/LOP251161

CSTR:32186.14.LOP251161

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology