Fig. 1. Flowchart of cell segmentation based on SPA

Fig. 2. Flowchart of automated analysis

Fig. 3. Schematic diagrams of effects by two cell segmentation methods. (a) Modified cell shaper method; (b) SPA-based method

Fig. 4. SRS images of RAW264.7 cells treated with different mass concentrations of LPS

Fig. 5. Analysis of cell morphology characteristics. (a) Clustered heatmap of SRS image features for different groups; (b) correlation coefficient matrix of characteristic parameters in the control group; (c) correlation coefficient matrix of characteristic parameters in the 1.0 μg/mL LPS-treated group; (d) statistical analysis of cell area (^**P<0.01); (e) statistical analysis of LD area; (f) statistical analysis of AR (^**P<0.01)

Fig. 6. Analysis results of LD in each group of cells. (a) SRS spectra; (b) ratio of intensity between 2855 cm^-1 and 2940 cm^-1 wave number in LD spectra(^****P < 0.0001,^#P < 0.05)