Researching | Research on three-dimensional culture and drug screening of tumor cell spheres based on microfluidic chips

Optical Instruments, Volume. 47, Issue 3, 37(2025)

Research on three-dimensional culture and drug screening of tumor cell spheres based on microfluidic chips

Zhiwei XUE and Lulu ZHENG^*

Author Affiliations

School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China

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References(14)

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[2] SIEGEL R L, MILLER K D, FUCHS H E et al. Cancer statistics, 2022[J]. CA: A Cancer Journal for Clinicians, 72, 7-33(2022).

[3] IMAMURA Y, MUKOHARA T, SHIMONO Y et al. Comparison of 2D- and 3D-culture models as drug-testing platforms in breast cancer[J]. Oncology Reports, 33, 1837-1843(2015).

[4] FANG Y, EGLEN R M. Three-dimensional cell cultures in drug discovery and development[J]. SLAS Discovery, 22, 456-472(2017).

[5] SCHUSTER B, JUNKIN M, KASHAF S S et al. Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids[J]. Nature Communications, 11, 5271(2020).

[6] VINCI M, GOWAN S, BOXALL F et al. Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation[J]. BMC Biology, 10, 29(2012).

[7] JUNG D J, SHIN T H, KIM M et al. A one-stop microfluidic-based lung cancer organoid culture platform for testing drug sensitivity[J]. Lab on A Chip, 19, 2854-2865(2019).

[8] GRACZ A D, WILLIAMSON I A, ROCHE K C et al. A high-throughput platform for stem cell niche co-cultures and downstream gene expression analysis[J]. Nature Cell Biology, 17, 340-349(2015).

[9] KARLSSON H, FRYKNÄS M, LARSSON R et al. Loss of cancer drug activity in colon cancer HCT-116 Cells during spheroid formation in a new 3-D spheroid cell culture system[J]. Experimental Cell Research, 318, 1577-1585(2012).

[10] GUMUSCU B, ALBERS H J, VAN DEN BERG A et al. Compartmentalized 3D tissue culture arrays under controlled microfluidic delivery[J]. Scientific Reports, 7, 3381(2017).

[11] BAPTISTA D, BIRGANI Z T, WIDOWSKI H et al. Polymer film-based microwell array platform for long-term culture and research of human bronchial organoids[J]. Materials Today Bio, 19, 100603(2023).

[12] BRANDENBERG N, HOEHNEL S, KUTTLER F et al. High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays[J]. Nature Biomedical Engineering, 4, 863-874(2020).

[13] SHI X H, LI Y G, YUAN Q Y et al. Integrated profiling of human pancreatic cancer organoids reveals chromatin accessibility features associated with drug sensitivity[J]. Nature Communications, 13, 2169(2022).

[14] LOPEZ J S, BANERJI U. Combine and conquer: challenges for targeted therapy combinations in early phase trials[J]. Nature Reviews Clinical Oncology, 14, 57-66(2017).

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Zhiwei XUE, Lulu ZHENG. Research on three-dimensional culture and drug screening of tumor cell spheres based on microfluidic chips[J]. Optical Instruments, 2025, 47(3): 37

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Paper Information

Received: Dec. 19, 2023

Accepted: --

Published Online: Jul. 28, 2025

The Author Email: Lulu ZHENG (llzheng@usst.edu.cn)

DOI:10.3969/j.issn.1005-5630.202312190137

Topics

laser devices and laser physics

Lasers and Laser Optics

laser manufacturing

Instrumentation, Measurement and Metrology