Chinese Journal of Lasers, Volume. 49, Issue 5, 0507201(2022)
Progress of Three-Dimensional, Label-Free Quantitative Imaging of Refractive Index in Biological Samples
Fig. 1. OPT forward measurement model. (a) Forward QPI measurement process; (b) illustration of Fourier-slice theorem
Fig. 4. Frequency domain mapping under different rotation modes. (a) Illustration of sample rotation; (b) illustration of incident wave rotation; (c) frequency domain mapping of one single measurement; (d) frequency domain mapping of sample rotation mode; (e) frequency domain mapping of incident wave rotation mode
Fig. 5. Comparison of three-dimensional refractive index distribution quantitative imaging results of hepatocyte cells[64]. (a) Direct inversion; (b) non-negativity constraint; (c) EP regularization; (d) TV regularization
Fig. 6. ODT measurement based on digital holography. (a) Sample rotation; (b) incident wave rotation
Fig. 7. Representative ODT imaging results based on interference measurement[129]. (a) Bright-field image of a normal colon tissue slice stained with H&E; (b) an unstained colon tissue slice imaged with ODT; (c)(e) three-dimensional refractive index quantitative images of 100 μm thick tissue slices, (c) pancreas, (d) large intestine, (e) small intestine
Fig. 8. Representative ODT imaging results based on non-interference measurement[82]. (a) Quantitative refractive index image of HeLa cells at z=2.93 μm; (b1)(b3) results of three HeLa cells at different depths; (c1)(c3) rendered results of three HeLa cells
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Zewen Yang, Lu Zhang, Ning Lü, Huijun Wang, Zhenxi Zhang, Li Yuan. Progress of Three-Dimensional, Label-Free Quantitative Imaging of Refractive Index in Biological Samples[J]. Chinese Journal of Lasers, 2022, 49(5): 0507201
Received: Nov. 30, 2021
Accepted: Jan. 10, 2022
Published Online: Mar. 9, 2022
The Author Email: Zhang Lu (gingerluzhang@mail.xjtu.edu.cn)