Chinese Journal of Lasers, Volume. 49, Issue 5, 0507201(2022)

Progress of Three-Dimensional, Label-Free Quantitative Imaging of Refractive Index in Biological Samples

Zewen Yang1, Lu Zhang1、*, Ning Lü1, Huijun Wang1, Zhenxi Zhang2, and Li Yuan3
Author Affiliations
  • 1State Key Laboratory for Manufacturing Systems Engineering, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, China
  • 2Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, China
  • 3Clinical Lab, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710061, China
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    Figures & Tables(8)
    OPT forward measurement model. (a) Forward QPI measurement process; (b) illustration of Fourier-slice theorem
    ODT forward measurement model
    Illustration of Fourier diffraction theorem
    Frequency domain mapping under different rotation modes. (a) Illustration of sample rotation; (b) illustration of incident wave rotation; (c) frequency domain mapping of one single measurement; (d) frequency domain mapping of sample rotation mode; (e) frequency domain mapping of incident wave rotation mode
    Comparison of three-dimensional refractive index distribution quantitative imaging results of hepatocyte cells[64]. (a) Direct inversion; (b) non-negativity constraint; (c) EP regularization; (d) TV regularization
    ODT measurement based on digital holography. (a) Sample rotation; (b) incident wave rotation
    Representative ODT imaging results based on interference measurement[129]. (a) Bright-field image of a normal colon tissue slice stained with H&E; (b) an unstained colon tissue slice imaged with ODT; (c)(e) three-dimensional refractive index quantitative images of 100 μm thick tissue slices, (c) pancreas, (d) large intestine, (e) small intestine
    Representative ODT imaging results based on non-interference measurement[82]. (a) Quantitative refractive index image of HeLa cells at z=2.93 μm; (b1)(b3) results of three HeLa cells at different depths; (c1)(c3) rendered results of three HeLa cells
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    Zewen Yang, Lu Zhang, Ning Lü, Huijun Wang, Zhenxi Zhang, Li Yuan. Progress of Three-Dimensional, Label-Free Quantitative Imaging of Refractive Index in Biological Samples[J]. Chinese Journal of Lasers, 2022, 49(5): 0507201

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    Paper Information

    Received: Nov. 30, 2021

    Accepted: Jan. 10, 2022

    Published Online: Mar. 9, 2022

    The Author Email: Zhang Lu (gingerluzhang@mail.xjtu.edu.cn)

    DOI:10.3788/CJL202249.0507201

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