Chinese Optics, Volume. 15, Issue 6, 1302(2022)
Wide-field-of-view and high-resolution HiLo optical sectioning microscopy system
Fig. 1. Design evaluation graphs of the wide-field-of-view and high-resolution objective
Fig. 2. Schematic diagram of wide-field-of-view and high-resolution HiLo optical sectioning microscopy system
Fig. 7. Comparison of brightfield imaging results of a HE-stained mouse brain slice illuminated by white light
Fig. 8. Comparison of 3D optical sectioning and wide-field fluorescence imaging results of a FITC-WGA-stained wheat seed fluorescence slice (50 slices, 2 μm thick of every slice)
Table 1. Design parameters of the wide-field-of-view and high-resolution objective
View tableView in ArticleTable 1. Design parameters of the wide-field-of-view and high-resolution objective
参数 指标 物方视场（Field of View, FOV） Φ6.0 mm 数值孔径(Numerical Aperture, NA) 0.5 工作距（Working Distance, WD） 2 mm 焦距（Focus, F） 40 mm 工作波段（Working Waveband） 436 ~ 800 nm 浸没介质（Immersion Medium） 空气
Table 2. Parameters of the system
View tableView in ArticleTable 2. Parameters of the system
参数 指标 照明光源 白光、488 nm/561 nm激光 成像视场 4.8 mm×3.6 mm（对角6.0 mm） 分辨率 横向0.74 μm，轴向4.16 μm 放大倍率 11.1 × 系统空间带宽积 151 M 工作模式 明场成像、宽场荧光成像、HiLo光切片成像 外形尺寸 565 mm（宽）×890 mm（长）×830 mm（高）
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Song LANG, Yan-wei ZHANG, Han-qing ZHENG, Lin-yu XU, Lu-han WANG, Yan GONG. Wide-field-of-view and high-resolution HiLo optical sectioning microscopy system[J]. Chinese Optics, 2022, 15(6): 1302
Category: Original Article
Received: Apr. 29, 2022
Accepted: Jul. 14, 2022
Published Online: Feb. 9, 2023
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