Chinese Journal of Lasers, Volume. 47, Issue 2, 207010(2020)
Optogenetics Based on Light-Gated Protein-Protein Interactions and Its Applications
Figures & Tables(7)
Fig. 1. Two types of light-gated protein-protein interaction widely used in optogenetics. (a) Hetero-dimerization; (b) homo-dimerization
Fig. 2. Scheme of light-induced conformational change in various photoactivatable proteins
Fig. 4. Schematic diagram of the Corelets system. Corelets system consists of two modules: a GFP-tagged ferritin core functionalized by 24 photoactivatable iLID domains and iLID's cognate partner, sspB, mCherry-tagged and conjugated to IDR. Dashed lines designate light-inducible hetero-dimerizing units
Fig. 5. Gather of light-induced active enzyme protein clusters, in which the input is shunted toward major product, and the production of unwanted byproduct is suppressed
Fig. 6. Basic mechanism optogenetic control of organelle transport and molecular motors
Table 1. Major parameters in optogenetics systems
View tableTable 1. Major parameters in optogenetics systems
Optogenetic system Wavelength Intensity Light source Illumination time Reference PhyB-PIF3 664 nm (ON), 748 nm (OFF) N/A LED array 5, 10, 30 min Ref. [7] CRY2 488 nm 25 μW 1 cm from object AOTF laser 15, 1 h, >4 h Ref. [12] VVD (LOV) 460 nm 0.84 W·m-2 LED lamp Patterned illumination for 24 h Ref. [33] LOV-ePDZ 465 nm 0.005 J·cm-2·s-1 Blue AlGaInP LEDs 1 s Ref. [26] IDR-CRY2 488 nm 0.02 μW, 0.06 μW, 1.5 μW Nikon A1 laser 1 s Ref. [34] IDR-iLid-SspB 488 nm 84 μW·μm-2 Nikon A1 laser 2.2 s Ref. [35] iLid-SspB 488 nm 0.1 μW Nikon A1 laser 1 s Ref. [36] LOV2-V19L 465 nm 53, 82 or 134 μmol·m-2·s-1 LED panel 8 h full light, or light pulses of 10 s on and 70 s off Ref. [37] CRY2 450nm 73 to 82 μmol·m-2·s-1 LED panel 5 min Ref. [38] CcaSR-pgi 520 nm (ON), 650 nm (OFF) 14.52 W·m-2 15.21 W·m-2 LED Continuous up to 10 h Ref. [39] CRY2-CIB1 488 nm 9.7 W·cm-2 LED, Leica DMI6000B microscope 200 ms pulse duration per 10 s Ref. [40] LOV-ePDZ 488 nm 10 W·cm-2 Nikon Eclipse TE2000E 100--150 ms Ref. [41]
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Huang Peiyuan, Song Yutong, Zhang Ning, Zhao Zhihao, Duan Liting. Optogenetics Based on Light-Gated Protein-Protein Interactions and Its Applications[J]. Chinese Journal of Lasers, 2020, 47(2): 207010
Paper Information
Category: biomedical photonics and laser medicine
Received: Oct. 28, 2019
Accepted: --
Published Online: Feb. 21, 2020
The Author Email: Duan Liting (ltduan@cuhk.edu.hk)
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