Fig. 1. Distribution of LMP-1 and VIM. (a) Brightfield image, (b) fluorescence image, and (c) merge of brightfield and fluorescence images of a single CNE1 cell expressing VIM-Cerulean construct; (d) brightfield image, (e) fluorescence image, and (f) merge of brightfield and fluorescence images of a single CNE1 cell expressing VIM-Cerulean construct; (g) fluorescence image, (h) fluorescence image ,and (i) co-localization fluorescence image of VIM-Cerulean and LMP-1-Venus in a single CNE1 cell co-expressing VIM-Cerulean and LMP-1-Vneus constructs

Fig. 2. Schematic diagram of VIM polarization

Fig. 3. Implementation of E-FRET for measuring FRET efficiency of VCV construct. (a) Fluorescence images of C32V construct in donor and acceptor channels before or after partially acceptor photobleaching; (b) fluorescence images of VCV construct in donor, acceptor and FRET channels, and distribution of FRET efficiency; (c) FRET efficiency histogram of 45 cells

Fig. 4. Schematic diagrams of LMP-1 and VIM interaction measured by FRET. (a) Distance between LMP-1 and VIM is less than 10 nm; (b) distance between LMP-1 and VIM is more than 10 nm

Fig. 5. Implementation of E-FRET for measuring interaction between LMP-1 and VIM. (a) Fluorescence images of CNE1 cells co-expressing VIM-Cerulean and LMP-1-Venus, and distribution of FRET efficiency; (b) corresponding FRET efficiency histogram of ROI-1; (c) corresponding FRET efficiency histogram of ROI-2; (d) corresponding FRET efficiency histogram of ROI-3

Fig. 6. Impact of rafts integrity on LMP-1 and VIM interaction. (a) Fluorescence images of LMP-1 and VIM, and distribution of FRET efficiency before or after treatment with MβCD; (b) statistical FRET efficiency of 30 ROIs from 10 cells before or after treatment with MβCD; (c) corresponding FRET efficiency histogram of 30 ROIs before treatment with MβCD; (d) corresponding FRET efficiency histogram of 30 ROIs after treatment with MβCD for 20 min

Fig. 7. Time-lapse imaging of LMP-1 and VIM interaction after treatment with MβCD. (a) Fluorescence images of VIM and distribution of FRET efficiency; (b) FRET efficiency of five ROIs at different time points

Fig. 8. Cytotoxicity induced by 40 mol/mL MβCD for 30 min in CNE1 and CNE1-LMP-1 cells