Fig. 1. Dynamic changes of blood indexes and the protocol of ⁹⁹Tc-MDP treatment of osteoporosis model(a) Blood calcium, (b) Blood phosphorus, (c) CTX-I, (d) P1NP, (e) Protocol of ⁹⁹Tc-MDP treatment

Fig. 2. Variation of bone density illustrated in imaging (a~d) For SPECT/CT at 4 weeks, (e) ⁹⁹Tc^m-MDP uptake of knee joint was quantified, (f~i) For SPECT/HRCT at 8 weeks, ⁹⁹Tc^m-MDP uptake (j) and CT values (k) of knee joint were quantified, where A is negative control group, B is model control group, C is ⁹⁹Tc-MDP treatment group, D is zoledronic acid treatment group

Fig. 3. Changes of blood calcium level (a) and blood phosphorus content (b) during the treatment of osteoporosis (color online) where A is negative control group, B is model control group, C is ⁹⁹Tc-MDP treatment group, D is zoledronic acid treatment group

Fig. 4. Diagrams of imbalance and re-balance of OB/OC (color online). The quantification of distance between green and red fluorescence (a), area of stained OB (b) and intensity of stained OB (c), and typical fluorescence, IHC staining of ALP and TRAP, and HE staining were summarized in (d), where A is negative control group, B is model control group, C is ⁹⁹Tc-MDP treatment group, D is zoledronic acid treatment group