Laser & Optoelectronics Progress, Volume. 62, Issue 18, 1817010(2025)
Event-Driven Microscopy: New Frontiers of Intravital Bioimaging (Invited)
Fig. 1. Imaging parameters and biological samples. (a) Trade-offs between various imaging parameters and biological specimens; (b) spatiotemporal scale distribution of different experimental targets, including neurons, cells, and tissues
Fig. 2. Comparison of the working principles of conventional optical microscope and event-driven microscope. (a) Structure of early conventional optical microscope; (b) workflow of event-driven microscope
Fig. 4. Event-driven mode-switching microscopy systems for imaging mitosis in cells. (a) Imaging workflow of Micropilot[36]; (b) Micropilot low-resolution confocal imaging of HeLa cells undergoing mitosis[36]; (c) high-resolution confocal imaging by Micropilot showing two-color 3D time-lapse sequences of HeLa cells in mitosis[36]; (d) imaging workflow of NanoJ-Fluidics[37]; (e) example of a rounding mitotic HeLa cell during live-cell imaging[37]; (f) NanoJ-Fluidics imaging of fixed cells undergoing mitosis[37]
Fig. 5. Working principle and application of event camera. (a) Trajectory of a moving ball in the
Fig. 6. etSTED imaging of neuronal activity[39]. (a) Schematic of synaptic vesicle dynamics during calcium signaling; (b) experimental timeline for a wide-field frame; (c) maximum projection analysis image from wide-field imaging detecting local calcium signals; (d) magnified views at two detected event locations; (e) 2.5 Hz etSTED time series; (f) synaptic vesicle cluster analysis; (g) schematic of endosomal vesicle interaction processes; (h) timeline schematic for a wide-field image; (i) tracking of vesicle movement in wide-field imaging; (j) magnified views of two representative events showing two tracked vesicles approaching each other; (k) triggered 2.8 Hz STED time series showing endosomal vesicle dynamics
Fig. 7. Tracking cancer cell migration using DDM and application of smartLLSM in the study of immune responses and nanomedicine. (a) Schematic of DDM applied for live feedback imaging of migratory subpopulations[41]; UMAP space colored by mean speed (b) and meandering index (c), revealing distinct migratory phenotypes[41]; (d) cells migrating at different speeds under low magnification (top) and rapidly migrating cells imaged under high magnification (bottom)[41]; (e) schematic of the smartLLSM imaging workflow[42]; (f) inverted fluorescence microscopy imaging of different CTL sample categories[42]; (g) representative maximum intensity projections and schematic diagrams of CTL synapses with lysosomal granules concentrated at the front, middle, and back[42]; (h) inverted fluorescence microscopy imaging of different sample categories of cells at various mitotic stages[42]; (i) maximum intensity projections of prometaphase and metaphase cells under control and 5 nmol/L paclitaxel treatment conditions[42]; (j) violin plot of metaphase plate angle for control and 5 nmol/L paclitaxel-treated cells in prometaphase and metaphase[42]
|
Get Citation
Copy Citation Text
Chenhui Yu, Guanyi Zhu, Fei He. Event-Driven Microscopy: New Frontiers of Intravital Bioimaging (Invited)[J]. Laser & Optoelectronics Progress, 2025, 62(18): 1817010
Category: Medical Optics and Biotechnology
Received: May. 7, 2025
Accepted: Jul. 1, 2025
Published Online: Sep. 11, 2025
The Author Email: Fei He (hefei@siom.ac.cn)
CSTR:32186.14.LOP251160