Fig. 1. Large FOV objective two-photon brain imaging^[12]. (a) Optical path diagram of the imaging system and objective lens (upper left); (b) two-photon imaging of neuronal cells activity of mice brain in vivo, the depth of imaging is 150 μm; (c) magnification of some details in white box of (b)

Fig. 2. Speckle light sheet illumination-based large FOV imaging system^[25]. (a) System setting, the speckle pattern formed by the laser through the ground glass disk is projected into the sample with a volume of 4.4 mm×3 mm×3 mm, forming a speckle light sheet with a thickness of about 3 μm for illumination; (b) Zebrafish whole body imaging with light sheet illumination; (c) Zebrafish whole body imaging with confocal microscopy

Fig. 3. Schematic diagram of microlenses arrangement and scanning direction

Fig. 4. Array microscopy system^[39]. (a) The optical setting of array microscopy; (b) high-throughput imaging of mouse kidney slices; (c) magnification of the rectangle in (b)

Fig. 5. Resolution enhanced lensless imaging system^[21]. (a) Schematic diagram of system design and the disordered surface; (b) high-throughput imaging of mouse kidney slices. Imaging results of three sub-regions b1, b2, b3 (red boxes) are compared with that of the fluorescence microscope using an objective with 20 X and 0.75 NA. The similarity is about 0.75