Ultrasensitive Detection of MicroRNA Based on Single Molecule Detection Principle

Jie Wu; Jialing Huang; Yue Wang; Zhenghao Li; Wenchao Zhou; Yihui Wu

doi:10.3788/AOS230453

Acta Optica Sinica, Volume. 43, Issue 13, 1317001(2023)

Ultrasensitive Detection of MicroRNA Based on Single Molecule Detection Principle

Jie Wu^1,2, Jialing Huang³, Yue Wang¹, Zhenghao Li^1,2, Wenchao Zhou^1、*, and Yihui Wu^1、**

Author Affiliations

¹Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 133033, Jilin, China

²University of Chinese Academy of Sciences, Beijing 100049, China

³School of Ophthalmology and Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China

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Figures & Tables(7)

Fig. 1. Schematic diagrams of miRNA detection principle. (a) miRNA compound formation; (b) beads immobilized in fibrin hydrogel; (c) miRNA single molecule counting

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Fig. 2. Fibrin hydrogel formation diagram

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Fig. 3. Fibrin hydrogel separation and fixation device

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Fig. 4. Single molecule counting algorithm processing (partial) results. (a) Partial processing results after open field screening; (b) partial processing results after dark field screening; (c) positive point processing results

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Fig. 5. miR-21 calibration curve

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Table 1. miR-21 experimental test results

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Table 1. miR-21 experimental test results

Concentration /（fmol/L）	Experimental time
Concentration /（fmol/L）	1	2	3	4	5
0	0.0401	0.0421	0.0322	0.0453	0.0251
2	0.0342	0.0415	0.0483	0.0441	0.0382
4	0.0586	0.0468	0.0449	0.0512	0.0392
6	0.0555	0.0511	0.0602	0.0545	0.0562
8	0.0643	0.0663	0.0633	0.0681	0.0657
10	0.0755	0.0725	0.0868	0.0746	0.0741

Table 2. R_AEB values for miR-21 calibration curves
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Table 2. R_AEB values for miR-21 calibration curves
Concentration /（fmol/L） Average R_AEB Standard deviation CV /%
0 0.0369 0.00820 22.2
2 0.0413 0.00541 13.1
4 0.0481 0.00726 15.1
6 0.0555 0.00328 5.9
8 0.0655 0.00185 2.9
10 0.0767 0.00575 7.5

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Jie Wu, Jialing Huang, Yue Wang, Zhenghao Li, Wenchao Zhou, Yihui Wu. Ultrasensitive Detection of MicroRNA Based on Single Molecule Detection Principle[J]. Acta Optica Sinica, 2023, 43(13): 1317001

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