Chinese Journal of Lasers, Volume. 47, Issue 2, 207016(2020)

Biomedical Photoacoustic Microscopy: Advances in Technology and Applications

Long Xiaoyun1,2 and Tian Chao1,2、*
Author Affiliations
  • 1School of Engineering Science, University of Science and Technology of China, Hefei, Anhui 230026, China
  • 2Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education Institutes, Hefei, Anhui 230026, China
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    Figures & Tables(10)
    Schematic of photoacoustic imaging[1]
    Typical embodiments of PAM[1,17]. (a) Transmission-mode OR-PAM; (b) reflection-mode OR-PAM; (c) dark-field AR-PAM; (d) schematic of typical OR-PAM
    Super-resolution PAM imaging[25-26]. (a) Photoimprint-PAM imaging of gold nanoparticles with a diameter of 150 nm[25]; (b) imaging of mitochondria in fibroblasts by conventional PAM[26]; (c) imaging of mitochondria in fibroblasts by super-resolution PAM; (d) imaging of mitochondria in fibroblasts by transmissi
    Principle of spatially invariant resolution photoacoustic microscopy[51]
    Integrated PAM/OCT for multi-modal chorioretinal imaging[4]. (a) System schematic; (b) fundus photo of retina vessels in New Zealand albino rabbit in vivo; (c) PAM image of retinal vessels; (d) three-dimensional rendered image of retinal vessels; (e) PAM image of choroidal vessels; (f) OCT B-scan of the fundus
    PAM images of a melanoma cell [21] and a red blood cell[103-104]in vitro. (a) PAM image of a melanoma cell[21]; (b) bright field microscopy image of a melanoma cell[21]; (c) fluorescence image of the cell nuclei superimposed with the PAM image[<xr
    Functional PAM imaging. (a) Sequential snapshots of single RBCs releasing oxygen in a mouse brain[114];(b) label-free multi-parameter PAM imaging in vivo[113]: total concentration of hemoglobin (CHb), sO2, blood flow, and profile of blood flow speed across the dashed line, respectively; (c) sO2 distribution in a mouse brain[<xref
    Cellular imaging using activatable photonic nanoclusters. (a) Fabrication of nanoclusters based on split green fluorescent protein (GFP) fragments; (b) highly-sensitive PAM images of U2OS cells based on nanoclusters
    • Table 1. Performance of OR-PAM and AR-PAM[6,20]

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      Table 1. Performance of OR-PAM and AR-PAM[6,20]

      CategoryLateral resolutionAxial resolutionPenetration depth
      OR-PAM0.51λONAOOne optical transport mean free path(~1 mm in soft tissue)
      AR-PAM0.71λANAA0.88cBA few optical transport mean free path
    • Table 2. Comparison of different scanning methods

      View table

      Table 2. Comparison of different scanning methods

      Ref.Scanning methodCo-axialTransducer focusingScanning speedField of vew /(mm×mm)Laser pulserepetition rate /kHz
      [56]MechanicalYesSpherical1 Hz/mm>10×10-
      [58]Voice-coilYesSpherical40 Hz/mm>5×54
      [60]MEMSYesSpherical50 Hz/B-scan9×410
      [64]NoSpherical270 Hz/mm0.15×0.15100
      [67]GalvanometerNoSylindrical50 Hz/B-scan40×40100
      [68]HybridYesSpherical250 Hz/B-scan4×850
      [55]MultifocalYesLinear array~20 Hz/mm6×51.35
      [70]Hexagon mirrorYesSpherical900 Hz/B-scan>12×10800
      [51]DMDYesSpherical0.05 Hz/volumetricscan0.18×0.181
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    Long Xiaoyun, Tian Chao. Biomedical Photoacoustic Microscopy: Advances in Technology and Applications[J]. Chinese Journal of Lasers, 2020, 47(2): 207016

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    Paper Information

    Category: biomedical photonics and laser medicine

    Received: Oct. 8, 2019

    Accepted: --

    Published Online: Feb. 21, 2020

    The Author Email: Chao Tian (ctian@ustc.edu.cn)

    DOI:10.3788/CJL202047.0207016

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