Fig. 1. Original Raman spectrum (curve 1) and preprocessed spectrum (curve 2) of single yeast cell

Fig. 2. Ethanol fermentation of S.cerevisiae strains Bp1, INVSc1, and W303a. (a) Growth curves; (b) consumption of glucose；(c) volume fraction of ethanol

Fig. 3. Averaged Raman spectra of single yeast cells at 6, 12, 24, 36, 58 h. (a) Bp1 strain; (b) INVSc1 strain; (c) W303a strain

Fig. 4. Resolution results of MCR-ALS of S.cerevisiae strain Bp1. (a) Recovered curves from single-cell Raman spectra; (b)－(f) relative volume fractions of individual cells corresponding to curves 1－5 in Fig. 4(a) respectively at different time points

Fig. 5. Resolution results of MCR-ALS of INVSc1 strain. (a) Recovered curves from single-cell Raman spectra; (b)－(f) relative volume fractions of individual cells corresponding to curves 1－5 in Fig. 5(a) respectively at different time points

Fig. 6. Resolution results of MCR-ALS of W303a strain. (a) Recovered curves from single-cell Raman spectra; (b)－(d) relative volume fractions of individual cells corresponding to curves 1－3 in Fig. 6(a) respectively at different time points