Chinese Journal of Lasers, Volume. 47, Issue 2, 207004(2020)

Optical Coherence Microscopy and Its Application

Han Tao, Qiu Jianrong, Wang Di, Meng Jia, Liu Zhiyi, and Ding Zhihua*
Author Affiliations
  • State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering,Zhejiang University, Hangzhou, Zhejiang 310027, China
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    Figures & Tables(19)
    Diagram of TD-OCM system and axial PSF[23]. (a) Schematic of TD-OCM system; (b) comparison of axial PSF under confocal and coherence-gated configuration of OCM system
    Schematic of FD-OCM system and comparison of sensitivity between TD-OCM and FD-OCM. (a) Schematic of FD-OCM system; (b) comparison of sensitivity between TD-OCM and FD-OCM with Gaussian source at 1300 nm[27]
    Overview of FF-OCM system
    Principle of data acquisition in FF-OCM system
    Illustrations of scanning imaging modes of PS-OCM and FF-OCM. (a) Scanning imaging mode of PS-OCM; (b) scanning imaging mode of FF-OCM
    Simulation of resolution varying with source central wavelength, bandwidth, and numerical aperture of objective in OCM. (a) Relation among axial resolution, source central wavelength, and bandwidth; (b) relation among lateral resolution, source central wavelength, and numerical aperture
    Ultrahigh resolution images of industrial sandpaper[38]. (a) Industrial sandpaper with particle size about 125 μm; (b) sandpaper longitudinal section image of general system; (c) sandpaper cross section image of ultra-high resolution system
    High-resolution images of onion epithelium obtained by FF-OCM system[29]
    Images of scattering samples without and with random phase[49].(a) Without random phase; (b) with random phase
    Axial resolution of OCM system versus detection depth and source bandwidth[13]. (a) Axial resolution versus FWHM of the Gaussian spectrum at different depths of human epidermis; (b) reachable minimum axial resolution versus depth of epidermis
    Diagram of dark-field OCM with extended depth of focus in sample arm
    En face images of murine pancreas at different depths obtained by extended-focus OCM system[68]. Scale bar: 200 μm. (a) 11 μm; (b) 54 μm; (c) 97 μm; (d) 110 μm
    Example of imaging process of human fingertip by GD-OCM[71]
    Cross-section images of African frog tadpole[70]. (a) Image acquired by GD-OCM system; (b) image acquired by OCM system at fixed focal plane
    Images of posterior layers of human corneas of healthy and FED corneas[91]. (a)(c)(d) Images of healthy cornea in the posterior elastic layer, endothelial cell layer, and posterior stromal layer; (b)(e)(f) images of FED cornea in the posterior elastic layer, endothelial cell layer, and posterior stromal layer
    Ex vivo images of cortical and subcortical structures in mouse brain slice[39]. (a) Transmission image showing imaging area; (b) result of Vis-OCM imaging; (c)(d) Vis-OCM images, fluorescence images of labeled amyloid plaques, and overlays of cortical and subcortical structures
    En face images of fibroblast obtained by dynamic detection[110]. Scale bar: 50 μm.(a) En face image; (b) fiber stretch detection; (c) cell migration detection
    Diagram of focus-extended OCM and angiograms of mouse cortex[109]. (a) Illustration of focus-extended system; (b)(c) interpolation process for angiogram; (d) angiogram of mouse cortex; (e)(f) quantitative distributions of total flow rate and axial flow rate
    • Table 1. Typical performance parameters of different imaging systems[88-90]

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      Table 1. Typical performance parameters of different imaging systems[88-90]

      CategoryCSLMOCTPS-OCMFF-OCM
      Axial resolution δz /μm0.5--20.5--80.5--80.5--8
      Lateral resolution δx /μm0.2--110--200.5--20.5--2
      Imaging depth zm /mm<0.21--3<0.2
      Field of view xm /mm2200--400>1000200--400200--400
      Imaging speed /(pixel·s-1)<5×107>1×1010>1×1010(2--5)×109
      Power P /μW~110--10010--1000.5--2
      Sensitivity RSN /dB<40100--110100--11080--90
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    Han Tao, Qiu Jianrong, Wang Di, Meng Jia, Liu Zhiyi, Ding Zhihua. Optical Coherence Microscopy and Its Application[J]. Chinese Journal of Lasers, 2020, 47(2): 207004

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    Paper Information

    Category: biomedical photonics and laser medicine

    Received: Oct. 8, 2019

    Accepted: --

    Published Online: Feb. 21, 2020

    The Author Email: Zhihua Ding (zh_ding@zju.edu.cn)

    DOI:10.3788/CJL202047.0207004

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