Advanced Photonics Nexus, Volume. 4, Issue 2, 026001(2025)

Fast full-color pathological imaging using Fourier ptychographic microscopy via closed-form model-based colorization

Yanqi Chen1,2、†, Jiurun Chen3, Zhiping Wang4, Yuting Gao1,2, Yonghong He3, Yishi Shi2, and An Pan1,2、*
Author Affiliations
  • 1Chinese Academy of Sciences, Xi’an Institute of Optics and Precision Mechanics, State Key Laboratory of Transient Optics and Photonics, Xi’an, China
  • 2University of Chinese Academy of Sciences, School of Optoelectronics, Beijing, China
  • 3Tsinghua University, Tsinghua Shenzhen International Graduate School, Shenzhen, China
  • 4Biozentrum, University of Basel, Basel, Switzerland
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    Figures & Tables(7)
    Principle of gCFPM. (a) Schematic of generalized dual-color-space-constrained model for color transfer; (b) principle of RGB-to-IHS transform; and (c) flow chart of gCFPM using IHS-RGB space constraints.
    Full-color imaging via gCFPM. (a) Imaging result of the sample; (b) photograph of cat stomach smooth muscle section; (c) enlarged view of ROI; and (d) full process time comparison of 3-FPM (three-channel FPM) and gCFPM.
    (a) Photograph of stratified epithelium section; (b) photograph of lycopodium sporophyll spike longitudinal section; (c) imaging result of (a); and (d) imaging result of (b).
    Comparison of different colorization methods. (a) Simulation result; (b) RMSE and CSSIM comparison in (a); and (c) comparison using experimental data. The upper row in (c) shows the result of ROI 3 in the stratified epithelium section; the lower row in (c) shows the result of ROI 1 in lycopodium sporophyll spike L.S.
    (a) Imaging results of gCFPM and 20× objective with defocus ranges from −30 to 30 μm; (b), (c) focus quality and sharpness assessment on two full-color imaging methods.
    • Table 1. Quantitative comparison of different methods in experiments.

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      Table 1. Quantitative comparison of different methods in experiments.

      Time (s)Stratified epithelium sectionLycopodium sporophyll spike L.S.
      SharpnessColorfulnessSharpnessColorfulness
      20 × 0.4NA17.59610.673324.25650.6535
      wavFPM22.950214.18530.695920.07680.5937
      FSCFPM3.719619.97780.730129.43960.5546
      CFFPM1622.744925.83840.640130.78400.5964
      gCFPM3.686820.34900.822028.57470.6435
    • Table 2. Assessment of gCFPM based on the extended linear transform.

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      Table 2. Assessment of gCFPM based on the extended linear transform.

      RMSE1-CSSIM
      IHS0.09280.1355
      YUV (BT.601)0.09260.1354
      YUV (BT.709)0.09270.1354
      YUV (BT.2020)0.09270.1354
      PKLT0.08910.1325
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    Yanqi Chen, Jiurun Chen, Zhiping Wang, Yuting Gao, Yonghong He, Yishi Shi, An Pan, "Fast full-color pathological imaging using Fourier ptychographic microscopy via closed-form model-based colorization," Adv. Photon. Nexus 4, 026001 (2025)

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    Paper Information

    Category: Research Articles

    Received: Oct. 25, 2024

    Accepted: Jan. 14, 2025

    Published Online: Mar. 21, 2025

    The Author Email: An Pan (panan@opt.cn)

    DOI:10.1117/1.APN.4.2.026001

    CSTR:32397.14.1.APN.4.2.026001

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