Chinese Journal of Lasers, Volume. 51, Issue 9, 0907013(2024)

Fully Integrated Nucleic Acid Analysis System Based on Syringe-Microfluidic Chip and Application of Precision Medicine

Qin Huang, Leyang Huang, Xiangyu Jin, Zeyin Mao, Anni Deng, Lü Wenqi, Hao Zhong, Shihong Wang, Yixuan Shi, Jiahui Chen, Shiguang Huang, and Guoliang Huang*
Author Affiliations
  • Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China
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    References(38)

    [1] Kevadiya B D, Machhi J, Herskovitz J et al. Diagnostics for SARS-CoV-2 infections[J]. Nature Materials, 20, 593-605(2021).

    [2] Zhao Y X, Chen F, Li Q et al. Isothermal amplification of nucleic acids[J]. Chemical Reviews, 115, 12491-12545(2015).

    [3] Wang C, Liu M, Wang Z F et al. Point-of-care diagnostics for infectious diseases: from methods to devices[J]. Nano Today, 37, 101092(2021).

    [4] Wu J, Huang J L, Wang Y et al. Ultrasensitive detection of microRNA based on single molecule detection principle[J]. Acta Optica Sinica, 43, 1317001(2023).

    [5] Udugama B, Kadhiresan P, Kozlowski H N et al. Diagnosing COVID-19: the disease and tools for detection[J]. ACS Nano, 14, 3822-3835(2020).

    [6] Hu F, Liu Y F, Li X C et al. Convenient nucleic acid detection method and point-of-care detection device based on CRISPR/Cas12a molecular diagnosis[J]. Chinese Journal of Lasers, 49, 1507402(2022).

    [7] Li N, Xu Y C, Cheng J. Review of the portable fully integrated nucleic acid analysis system[J]. Chinese Journal of Biomedical Engineering, 41, 602-613(2022).

    [8] Yan L, Zhou J, Zheng Y et al. Isothermal amplified detection of DNA and RNA[J]. Molecular BioSystems, 10, 970-1003(2014).

    [9] Xi J Y, Yu G X, Qian X J et al. Advances in the laboratory diagnosis for COVID-19 virus[J]. Journal of Molecular Diagnostics and Therapy, 12, 265-269(2020).

    [10] Xie C M, Wu H P, Ma X P et al. New molecular diagnostic technologies for clinical detection of SARS-CoV-2[J]. Hereditas(Beijing), 42, 870-881(2020).

    [11] Reyes D R, van Heeren H, Guha S et al. Accelerating innovation and commercialization through standardization of microfluidic-based medical devices[J]. Lab on a Chip, 21, 9-21(2021).

    [12] Arora A, Simone G, Salieb-Beugelaar G B et al. Latest developments in micro total analysis systems[J]. Analytical Chemistry, 82, 4830-4847(2010).

    [13] Suárez G, Jin Y H, Auerswald J et al. Lab-on-a-chip for multiplexed biosensing of residual antibiotics in milk[J]. Lab on a Chip, 9, 1625-1630(2009).

    [14] Govindarajan A V, Ramachandran S, Vigil G D et al. A low cost point-of-care viscous sample preparation device for molecular diagnosis in the developing world; an example of microfluidic origami[J]. Lab on a Chip, 12, 174-181(2012).

    [15] Baek S H, Park C, Jeon J et al. Three-dimensional paper-based microfluidic analysis device for simultaneous detection of multiple biomarkers with a smartphone[J]. Biosensors, 10, 187(2020).

    [16] Nevenzal H, Noach-Hirsh M, Skornik-Bustan O et al. A high-throughput integrated microfluidics method enables tyrosine autophosphorylation discovery[J]. Communications Biology, 2, 42(2019).

    [17] Huang G L, Huang Q, Xie L et al. A rapid, low-cost, and microfluidic chip-based system for parallel identification of multiple pathogens related to clinical pneumonia[J]. Scientific Reports, 7, 6441(2017).

    [18] Ruiz R A, Gonzalez J L, Vazquez-Alvarado M et al. Beyond wax printing: fabrication of paper-based microfluidic devices using a thermal transfer printer[J]. Analytical Chemistry, 94, 8833-8837(2022).

    [19] Dong B, Guo L H, Liu D Y et al. Progress in tumor biomarker detection based on fluorescence method[J]. Chinese Journal of Lasers, 49, 2007103(2022).

    [20] Pinsky B A, Sahoo M K, Sandlund J et al. Analytical performance characteristics of the Cepheid GeneXpert Ebola assay for the detection of Ebola virus[J]. PLoS One, 10, e0142216(2015).

    [21] Boehme C C, Nabeta P, Hillemann D et al. Rapid molecular detection of tuberculosis and rifampin resistance[J]. The New England Journal of Medicine, 363, 1005-1015(2010).

    [22] N'guessan K K, Riccardo A, Dutoziet C C et al. Genotyping of mutations detected with GeneXpert[J]. International Journal of Mycobacteriology, 5, 142-147(2016).

    [24] Pulido M R, Moreno-Martínez P, González-Galán V et al. Application of BioFire FilmArray Blood Culture Identification panel for rapid identification of the causative agents of ventilator-associated pneumonia[J]. Clinical Microbiology and Infection, 24, 1213.e1-1213.e4(2018).

    [25] Lawson S K, Chung D, Das S et al. Analytical and workflow evaluation of the ARIES sample-to-result molecular assay for Clostridium difficile[J]. Annals of Clinical and Laboratory Science, 48, 168-176(2018).

    [26] Sieker J T, Horowitz C, Hu C T K et al. Analytic sensitivity of 3 nucleic acid detection assays in diagnosis of SARS-CoV-2 infection[J]. The Journal of Applied Laboratory Medicine, 6, 421-428(2021).

    [27] Mullis K, Faloona F, Scharf S et al. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction[J]. Cold Spring Harbor Symposia on Quantitative Biology, 51, 263-273(1986).

    [28] Dong X B, Liu L Y, Tu Y P et al. Rapid PCR powered by microfluidics: a quick review under the background of COVID-19 pandemic[J]. Trends in Analytical Chemistry: TRAC, 143, 116377(2021).

    [29] Compton J. Nucleic acid sequence-based amplification[J]. Nature, 350, 91-92(1991).

    [30] Lizardi P M, Huang X, Zhu Z et al. Mutation detection and single-molecule counting using isothermal rolling-circle amplification[J]. Nature Genetics, 19, 225-232(1998).

    [31] Murakami T, Sumaoka J, Komiyama M. Sensitive isothermal detection of nucleic-acid sequence by primer generation-rolling circle amplification[J]. Nucleic Acids Research, 37, e19(2009).

    [32] Walker G T, Little M C, Nadeau J G et al. Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system[J]. Proceedings of the National Academy of Sciences of the United States of America, 89, 392-396(1992).

    [33] Vincent M, Xu Y, Kong H M. Helicase-dependent isothermal DNA amplification[J]. EMBO Reports, 5, 795-800(2004).

    [34] Notomi T, Okayama H, Masubuchi H et al. Loop-mediated isothermal amplification of DNA[J]. Nucleic Acids Research, 28, E63(2000).

    [35] Piepenburg O, Williams C H, Stemple D L et al. DNA detection using recombination proteins[J]. PLoS Biology, 4, e204(2006).

    [36] Xing W L, Wang J D, Zhao C et al. A highly automated mobile laboratory for on-site molecular diagnostics in the COVID-19 pandemic[J]. Clinical Chemistry, 67, 672-683(2021).

    [37] You Q M, Hu L, Qi C et al. Nucleic acid integrated detection method and detection reagent tube[P].

    [38] Thatcher S A. DNA/RNA preparation for molecular detection[J]. Clinical Chemistry, 61, 89-99(2015).

    [39] Lin X, Bo Z H, Lü W Q et al. Miniaturized microfluidic-based nucleic acid analyzer to identify new biomarkers of biopsy lung cancer samples for subtyping[J]. Frontiers in Chemistry, 10, 946157(2022).

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    Qin Huang, Leyang Huang, Xiangyu Jin, Zeyin Mao, Anni Deng, Lü Wenqi, Hao Zhong, Shihong Wang, Yixuan Shi, Jiahui Chen, Shiguang Huang, Guoliang Huang. Fully Integrated Nucleic Acid Analysis System Based on Syringe-Microfluidic Chip and Application of Precision Medicine[J]. Chinese Journal of Lasers, 2024, 51(9): 0907013

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    Paper Information

    Category: biomedical photonics and laser medicine

    Received: Dec. 1, 2023

    Accepted: Feb. 26, 2024

    Published Online: Apr. 26, 2024

    The Author Email: Huang Guoliang (tshgl@tsinghua.edu.cn)

    DOI:10.3788/CJL231461

    CSTR:32183.14.CJL231461

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