Chinese Journal of Lasers, Volume. 51, Issue 9, 0907013(2024)

Fully Integrated Nucleic Acid Analysis System Based on Syringe-Microfluidic Chip and Application of Precision Medicine

Qin Huang, Leyang Huang, Xiangyu Jin, Zeyin Mao, Anni Deng, Lü Wenqi, Hao Zhong, Shihong Wang, Yixuan Shi, Jiahui Chen, Shiguang Huang, and Guoliang Huang*
Author Affiliations
  • Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China
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    Figures & Tables(9)
    Structure and sectional view of the nucleic acid extraction syringe and microfluidic chip detection system
    Schematic diagram of nucleic acid extraction process by a syringe structure nucleic acid extraction system
    Design, physical pictures and working principle of the microfluidic chip. (a) Design of the substrate and primer embedding sequence; (b) physical picture of the microfluidic chip and its working principle. C. alb-L, C. gla-L, C. par-L, and C. tro-L represent the types of primers embedded in the reaction chamber. LAMP reaction reagent is transparent in real, and red pigment is added here to indicate the location of the amplification reagent on the chip. PC, positive control; NC, negative control; No., serial number
    Structural diagram and physical picture of the temperature control module. (a) Hierarchical diagram of the temperature control module; (b) structural diagram of the temperature control module; (c) physical picture of the temperature control module
    Structural diagram and physical picture of the fluorescence detection module. (a) Structural diagram; (b) physical picture
    Diagram of microfluidic chip nucleic acid amplification and detection system
    Physical picture of syringe-microfluidic chip based nucleic acid extraction and detection system
    • Table 1. Detection analysis of the fully integrated nucleic acid analysis system versus kit method

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      Table 1. Detection analysis of the fully integrated nucleic acid analysis system versus kit method

      Detection parameterContent
      Fully integrated systemKit method
      Manual steps110+3
      Time consuming /min10~60
      Average Ct value /min20.924.4
      Standard deviation(SD)/min1.31.5
      Coefficient of variation(CV)value /%6.226.14
    • Table 2. Comparison of clinical test results of fully integrated method and culture method for four fungi

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      Table 2. Comparison of clinical test results of fully integrated method and culture method for four fungi

      Test MethodPathogenCulture methodTotalPχ2

      Kappa

      value

      C. albC.glaC.parC.troNegative

      method

      Fully integrated

      C.alb2300002310.950
      C.gla080008
      C.par001001
      C.tro000404
      Negative20002628
      Total258142664
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    Qin Huang, Leyang Huang, Xiangyu Jin, Zeyin Mao, Anni Deng, Lü Wenqi, Hao Zhong, Shihong Wang, Yixuan Shi, Jiahui Chen, Shiguang Huang, Guoliang Huang. Fully Integrated Nucleic Acid Analysis System Based on Syringe-Microfluidic Chip and Application of Precision Medicine[J]. Chinese Journal of Lasers, 2024, 51(9): 0907013

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    Paper Information

    Category: biomedical photonics and laser medicine

    Received: Dec. 1, 2023

    Accepted: Feb. 26, 2024

    Published Online: Apr. 26, 2024

    The Author Email: Huang Guoliang (tshgl@tsinghua.edu.cn)

    DOI:10.3788/CJL231461

    CSTR:32183.14.CJL231461

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