Chinese Journal of Lasers, Volume. 51, Issue 9, 0907013(2024)
Fully Integrated Nucleic Acid Analysis System Based on Syringe-Microfluidic Chip and Application of Precision Medicine
Fig. 1. Structure and sectional view of the nucleic acid extraction syringe and microfluidic chip detection system
Fig. 2. Schematic diagram of nucleic acid extraction process by a syringe structure nucleic acid extraction system
Fig. 3. Design, physical pictures and working principle of the microfluidic chip. (a) Design of the substrate and primer embedding sequence; (b) physical picture of the microfluidic chip and its working principle. C. alb-L, C. gla-L, C. par-L, and C. tro-L represent the types of primers embedded in the reaction chamber. LAMP reaction reagent is transparent in real, and red pigment is added here to indicate the location of the amplification reagent on the chip. PC, positive control; NC, negative control; No., serial number
Fig. 4. Structural diagram and physical picture of the temperature control module. (a) Hierarchical diagram of the temperature control module; (b) structural diagram of the temperature control module; (c) physical picture of the temperature control module
Fig. 5. Structural diagram and physical picture of the fluorescence detection module. (a) Structural diagram; (b) physical picture
Fig. 6. Diagram of microfluidic chip nucleic acid amplification and detection system
Fig. 7. Physical picture of syringe-microfluidic chip based nucleic acid extraction and detection system
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Qin Huang, Leyang Huang, Xiangyu Jin, Zeyin Mao, Anni Deng, Lü Wenqi, Hao Zhong, Shihong Wang, Yixuan Shi, Jiahui Chen, Shiguang Huang, Guoliang Huang. Fully Integrated Nucleic Acid Analysis System Based on Syringe-Microfluidic Chip and Application of Precision Medicine[J]. Chinese Journal of Lasers, 2024, 51(9): 0907013
Category: biomedical photonics and laser medicine
Received: Dec. 1, 2023
Accepted: Feb. 26, 2024
Published Online: Apr. 26, 2024
The Author Email: Huang Guoliang (tshgl@tsinghua.edu.cn)
CSTR:32183.14.CJL231461