Genetic evolution of 16S rRNA in <i>Escherichia coli</i> induced by low-energy N<sup>+</sup> implantation

Chao TANG; Ting WANG; Xuerui WANG; Minghui CHEN; Changlong CAI

doi:10.11889/j.1000-3436.2023-0084

Journal of Radiation Research and Radiation Processing, Volume. 42, Issue 1, 010301(2024)

Genetic evolution of 16S rRNA in Escherichia coli induced by low-energy N⁺ implantation

Chao TANG¹, Ting WANG², Xuerui WANG¹, Minghui CHEN³, and Changlong CAI^1、*

Author Affiliations

¹School of Optoelectronic Engineering, Xi'an University of Technology, Xi'an 710021, China

²College of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China

³China Northern Vehicle Research Institute, Beijing 100072, China

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Figures & Tables(9)

Fig. 1. Structure of 16S rRNA gene in antibiotic-resistant E.coli byN⁺ ion implantation

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Fig. 2. Phylogenetic tree of antibiotic-resistant E.coli byN⁺ implantation

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Fig. 3. Prediction of 2D structure of C region of antibiotic-resistant E.coli byN⁺ implantation

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Fig. 4. Prediction of 2D structure of V region of antibiotic-resistant E.coli byN⁺ implantation

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Fig. 5. Venn analysis of antibiotic-resistant genes in antibiotic-resistant E.coli

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Fig. 6. Antibiotic-resistant analysis of 16S rRNA antibiotic-resistant E.coli

PB: polymyxin B, CIP: ciprofloxacin, OFX: ofloxacin, NOR: norfloxacin, LEV: levofloxacin, N: neomycin, AK: amikacin, GM: gentamicin, K: kanamycin, CTR: ceftriaxone, CAZ: ceftazidime, CXM: cefuroxime, CZ: cefazolin, CB: carbenicillin, AM: ampicillin, tetracycline: TE, minocycline: MI, doxycycline: DX, C: chloramphenicol, FZ: furazolidone

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Table 1. Mutation survival rate and mutation rate of antibiotic-resistant E.coli byN⁺ implantation

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Table 1. Mutation survival rate and mutation rate of antibiotic-resistant E.coli byN⁺ implantation

组别 Groups	存活菌株 Survival number	存活率 / % Survival rate	耐药菌株数（突变率 / %） Number of antibiotic-resistant E.coli (mutation rate)
组别 Groups	存活菌株 Survival number	存活率 / % Survival rate	NOR+	PB+	SM+
1	1 728	17.28	4（0.231）	2（0.116）	0
2	1 472	14.72	0	7（0.476）	0
3	1 597	15.97	0	2（0.125）	10（0.626）
合计 Total	4 797	15.99	4（0.083）	11（0.229）	10（0.208）
对照组Control group	39 042	96.8	0	1(0.003)	0

Table 2. Characteristics of 16S rRNA gene in antibiotic-resistant E.coli byN⁺ ion implantation

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Table 2. Characteristics of 16S rRNA gene in antibiotic-resistant E.coli byN⁺ ion implantation

组别 Groups	长度 / bp Length	同源性 / % Homology	耐药菌株比例 / % Proportion of antibiotic- resistant E.coli	NOR+株比例 / % Proportion of NOR+	PB+株比例 / % Proportion of PB+	SM+株比例 / % Proportion of SM+
ATCC25922	1 530	100	80 （20/25）	4（1/25）	40（10/25）	36（9/25）
EC-N4	1 264	99.5	4（1/25）	4（1/25）	0	0
EC-N32	908	99.4	4（1/25）	4（1/25）	0	0
EC-N35	1 444	99.5	4（1/25）	4（1/25）	0	0
ECD-S34	1 254	99.5	4（1/25）	0	0	4（1/25）
ECD-P9	1 459	99.6	4（1/25）	0	4（1/25）	0

Table 3. GC% analysis of 16S rRNA gene of antibiotic-resistant E.coli byN⁺ ion implantation

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Table 3. GC% analysis of 16S rRNA gene of antibiotic-resistant E.coli byN⁺ ion implantation

类别 Groups	A%	T%	C%	G%	CG%
ATCC25922	25.10	20.20	31.83	22.88	54.71
EC-N4	25.00	20.17	31.57	23.26	54.83
EC-N32	25.00	19.38	32.93	22.69	55.62
EC-N35	24.93	20.08	31.99	22.99	54.99
ECD-S34	25.28	19.86	32.30	22.57	54.86
ECD-P9	24.81	20.29	31.94	22.96	54.90

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Chao TANG, Ting WANG, Xuerui WANG, Minghui CHEN, Changlong CAI. Genetic evolution of 16S rRNA in Escherichia coli induced by low-energy N⁺ implantation[J]. Journal of Radiation Research and Radiation Processing, 2024, 42(1): 010301

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Paper Information

Category: Research Articles

Received: Oct. 4, 2023

Accepted: Nov. 14, 2023

Published Online: Mar. 27, 2024

The Author Email: CAI Changlong (蔡长龙)

DOI:10.11889/j.1000-3436.2023-0084

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Instrumentation, Measurement and Metrology

Table 1. Mutation survival rate and mutation rate of antibiotic-resistant E.coli byN+ implantation

Table 1. Mutation survival rate and mutation rate of antibiotic-resistant E.coli byN+ implantation

Table 2. Characteristics of 16S rRNA gene in antibiotic-resistant E.coli byN+ ion implantation

Table 2. Characteristics of 16S rRNA gene in antibiotic-resistant E.coli byN+ ion implantation

Table 3. GC% analysis of 16S rRNA gene of antibiotic-resistant E.coli byN+ ion implantation

Table 3. GC% analysis of 16S rRNA gene of antibiotic-resistant E.coli byN+ ion implantation

Table 1. Mutation survival rate and mutation rate of antibiotic-resistant E.coli byN⁺ implantation

Table 1. Mutation survival rate and mutation rate of antibiotic-resistant E.coli byN⁺ implantation

Table 2. Characteristics of 16S rRNA gene in antibiotic-resistant E.coli byN⁺ ion implantation

Table 2. Characteristics of 16S rRNA gene in antibiotic-resistant E.coli byN⁺ ion implantation

Table 3. GC% analysis of 16S rRNA gene of antibiotic-resistant E.coli byN⁺ ion implantation

Table 3. GC% analysis of 16S rRNA gene of antibiotic-resistant E.coli byN⁺ ion implantation