Spectroscopy and Spectral Analysis, Volume. 41, Issue 2, 419(2021)
Integrated Detection of Foodborne Pathogens by In-Situ Infrared Spectroscopy Based on ZnSe Film Transmission Method
Figures & Tables(7)
Fig. 1. Diagram (including sample pretreatment) of ZnSe film transmission and KBr pressed pellet method
Fig. 2. Integrated infrared macroscopic fingerprint of four foodborne pathogens based on ZnSe film transmission method
Fig. 3. Comparison of IR spectra in four foodborne pathogens based on ZnSe film transmission and KBr pressed pellet method
(a):
Fig. 4. SD-IR spectra of
Fig. 5. Comparison of SD-IR comparison spectra in four foodborne pathogens based on ZnSe film transmission and KBr pressed pellet method (1 500~900 cm-1)
(a):
Table 1. Major spectral band assignments for Fourier transform infrared spectroscopy (FT-IR) of four foodborne pathogens
View tableView in ArticleTable 1. Major spectral band assignments for Fourier transform infrared spectroscopy (FT-IR) of four foodborne pathogens
Wavenumber/cm-1 Spectral region Band assignment 3 000~2 800 Lipids ν as/s(CH3),ν as/s(CH2)1 800~1 500 Proteins and peptides Amide Ⅰ, Amide Ⅱ 1 500~1 200 A mixed region δ s(CH2),ν s(COO-), Amide Ⅲ, v85(P=O)1 200~900 Polysaccharides C—O—C, C—O ring vibrations 900~700 A fingerprint region -
Table 2. Comparison of characteristic peak in SD-IR spectra (1 500~900 cm-1) based on ZnSe film transmission and KBr pressed pellet method
View tableView in ArticleTable 2. Comparison of characteristic peak in SD-IR spectra (1 500~900 cm-1) based on ZnSe film transmission and KBr pressed pellet method
Bacterial strains ZnSe film transmission method KBr pressed pellet method Number of
labeled peaksNumber of
strong peaksNumber of
twin peaksNumber of
labeled peaksNumber of
strong peaksNumber of
twin peaksE. coli DH5α19 12 4 12 - - S. enteritidis CMCC 5004120 8 3 14 - - V. cholerae SH0422 7 2 17 3 1 S. aureus SH1020 11 3 15 3 -
Tools
Get Citation
Copy Citation Text
Yan-yan LIU, Ning-ping TAO, Xi-chang WANG, Ying LU, Chang-hua XU. Integrated Detection of Foodborne Pathogens by In-Situ Infrared Spectroscopy Based on ZnSe Film Transmission Method[J]. Spectroscopy and Spectral Analysis, 2021, 41(2): 419
Paper Information
Category: Research Articles
Received: Jan. 13, 2020
Accepted: Apr. 15, 2020
Published Online: Apr. 8, 2021
The Author Email: LIU Yan-yan (431036615@qq.com)
© Copyright 2018-2021 | Chinese Laser Press.
All Rights Reserved 沪ICP备15018463号-20