Fig. 1. Schematic diagram of CRISPR/dCas9 mediated fluorescent protein based on imaging^[19]. (a) dCas9-FP fusion protein; (b) MCP-MS2-FP system: the MCP aptamer fused with fluorescent protein binds to the modified sgRNA MS2 stem ring; (c) Simultaneous application of dCas9-GFP and PP7-PCP-RFP systems: multicolor imaging of multiple gene loci

Fig. 2. CRISPR/Cas9 fluorescent imaging of living cells^[13]. (a) CRISPR labeling of the nonrepetitive region of MUC4 intron using multiple optimized sgRNAs; (b) CRISPR imaging of telomeres in RPE cells (scale bar 5 μm) and its trajectory tracing diagram (scale bar 200 nm); (c) MUC4 image of HeLa cell during mitosis

Fig. 3. Schematic diagram of CRISPR/dCas9 mediated organic dye based imaging^[19]. (a) dCas9-HaloTag fusion protein; (b) sgRNA-Bro-ccoli/DFHBI-1T system: modified sgRNA is used to bind one or more RNA aptamers DFHBI-1T; (c) sgRNA-MTS-MB system: molecular biomarker MB combines with nucleic acid target MTS to stimulate fluorescence

Fig. 4. Schematic diagram of CRISPR/dCas9 mediated quantum dots (QDs) based imaging^[19]. (a) LplA-mediated quantum dots system: dCas9 combines with TCO2 and reacts with TZ1-QD via LplA; (b) BirA-mediated quantum dots system: BirA-mediated dCas9 biotinylated and combines with SA-QD