Automatic Background Recognition and Data Selection for Online Quantitative E-FRET Imaging

Han Sun; Tongsheng Chen

doi:10.3788/AOS201939.0217001

Acta Optica Sinica, Volume. 39, Issue 2, 0217001(2019)

Automatic Background Recognition and Data Selection for Online Quantitative E-FRET Imaging

Han Sun^1,2、* and Tongsheng Chen^2、*

¹ School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou, Guangdong 510006, China

² College of Biophotonics, South China Normal University, Guangzhou, Guangdong 510631, China

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Figures & Tables(7)

Fig. 1. Statistical pixel grayscale values of images and corrected images

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Fig. 2. Quantitative E and RC. (a) Images; (b) corresponding histograms

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Fig. 3. E and RC results after data filtering with different Boolean logic templates. (a) Bmask; (b) E; (c) RC

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Fig. 4. E and RC images obtained by online dynamic detection

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Fig. 5. E and RC values according to Fig. 4

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Table 1. E and RC after template filtering with different β
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Table 1. E and RC after template filtering with different β
β E R_C
Mean SD Mean SD
0 0.2835 0.1146 1.12 1.15
1 0.3187 0.0363 1.13 0.07
2 0.3019 0.0293 1.14 0.05
3 0.3123 0.0266 1.14 0.04
Notes: reported E^[16] is 0.299±0.004, and expected R_C is 1.

Table 2. E-FRET quantitative imaging results of different plasmids

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Table 2. E-FRET quantitative imaging results of different plasmids

Plasmid	β	E				R_C
Plasmid	β	Mean value	SD	Reported value		Mean value		SD	Expected value
C4Y	0	0.3200	0.1144	0.299±0.004			1.28	0.96	1
	1	0.3165			0.0396		1.14		0.08
	2	0.2911			0.0277		1.13		0.07
C10Y	0	0.2391	0.0736	0.223±0.003			1.27	0.70	1
	1	0.2301			0.0346		1.15		0.11
	2	0.2206			0.0382		1.09		0.09
C40Y	0	0.1430	0.0851	0.158±0.002			1.13	0.91	1
	1	0.1444			0.0362		1.10		0.13
	2	0.1486			0.0319		1.09		0.10
C80Y	0	0.0907	0.0534	0.116±0.002			1.23	0.92	1
	1	0.0840			0.0215		1.10		0.13
	2	0.0852			0.0196		1.08		0.07

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Han Sun, Tongsheng Chen. Automatic Background Recognition and Data Selection for Online Quantitative E-FRET Imaging[J]. Acta Optica Sinica, 2019, 39(2): 0217001

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