Chinese Journal of Lasers, Volume. 41, Issue 12, 1215003(2014)

Real-Time Detection on the Expression of Soluble Protein and Inclusion Body in the Recombinant Escherichia coli with Laser Tweezers Raman Spectroscopy

Huang Shushi1,2、*, Lu Mingqian2, Li Bing3, Zhou Bing1, and Chen Limei1
Author Affiliations
  • 1[in Chinese]
  • 2[in Chinese]
  • 3[in Chinese]
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    References(28)

    [1] [1] Z Y Li, C P Liu, L Q Zhu, et al.. The chaperone activity of trigger factor is distinct from its isomerase activity during co-expression with adenylate kinase in Escherichia coli[J]. FEBS Lett, 2001, 506(2): 108-112.

    [2] [2] H Lilie, E Schwarz, R Rudolph. Advances in refolding of proteins produced in E. coli[J]. Curr Opin Biotech, 1998, 9(5): 497-501.

    [3] [3] M Gribskov, R R Burgess. Overexpression and purification of the sigma subunit of Escherichia coli RNA polymerase[J]. Gene, 1983, 26(2): 109-118.

    [4] [4] Zhou Ying, Li Bingying, Li Xuebao. Roles of 14-3-3 proteins in regulating plant development[J]. Chinese Bulletin of Botany, 2012, 47(1): 55-64.

    [5] [5] Wu Jiafa, Wu Qiao. Regulatory mechanism and biological function of 14-3-3 protein family[J]. Chinese Journl of Cell Biology, 2005, 27(2): 101-104.

    [6] [6] Lu Mingqian, Dong Rong, Wen Shunhua, et al.. Detection of formate dehydrogenase protein expression in single living Escherichia coli cells by laser tweezers Raman spectroscopy[J]. Chinese Journal of Analytical Chemistry, 2012, 40(12): 1845-1851.

    [7] [7] C Xie, Y Li. Confocal micro-Raman spectroscopy of single biological cells using optical trapping and shifted excitation difference techniques[J]. J Appl Phys, 2003, 93(5): 2982-2986.

    [8] [8] W Huang, A D Ward, A S Whiteley. Raman tweezers sorting of single microbial cells[J]. Env Microbiol Rep, 2009, 1(1): 44-49.

    [9] [9] J W Chan, H Winhold, M H Corzett, et al.. Monitoring dynamic protein expression in living E. coli bacterial cells by laser tweezers Raman spectroscopy[J]. Cytom Part A, 2007, 71(7): 468-474.

    [10] [10] C Xie, N Nguyen, Y Zhu, et al.. Detection of the recombinant proteins in single transgenic microbial cell using laser tweezers and Raman spectroscopy[J]. Anal Chem, 2007, 79(24): 9269-9275.

    [11] [11] Zhou Bing, Lu Mingqian, Zhao Liwei, et al.. Detection of expression of broad bean 14-3-3b soluble and inclusion body protein in single living Escherichia coli cell by laser tweezers Raman spectroscopy[J]. Chinese Journal of Analytical Chemistry, 2013, 41(12): 1789-1794.

    [12] [12] Qin Zhaojun, Lai Junzhuo, Liu Bin, et al.. Raman spectroscopic analysis of ethanol fermentation at various initial pH levels[J]. Chinese J Lasers, 2013, 40(2): 0215001.

    [13] [13] C Allen, S Büttner, A D Aragon, et al.. Isolation of quiescent and nonquiescent cells from yeast stationary-phase cultures[J]. J Cell Biol, 2006, 174(1): 89-100.

    [14] [14] Sun Meijuan, Jiang Yuling, Lai Aihua, et al.. Analysis of lipid and carotenoids in rhodosporidium toruloides using laser tweezer Raman spectroscopy[J]. Laser & Optoelectronics Progress, 2013, 50(3): 033001.

    [17] [17] W S Craig, B P Gaber. Laser Raman scattering from an enzyme of well-documented structure, human carbonic anhydrase B[J]. J Am Chem Soc, 1977, 99(12): 4130-4134.

    [18] [18] Luo Xia, Yang Zhirong, Zhu Wen, et al.. Studies on structure of Pseudomonas pseudoalcaligenes insecticidal protein[J]. Progress in Biochemistry and Biophysics, 2000, 27(1): 68-71.

    [19] [19] G Shetty, C Kendall, N Shepherd, et al.. Raman spectroscopy: Elucidation of biochemical changes in carcinogenesis of oesophagus[J]. Brit J Cancer, 2006, 94(10): 1460-1464.

    [20] [20] I Notingher. Raman spectroscopy cell-based biosensors[J]. Sensors, 2007, 7(8): 1343-1358.

    [21] [21] Niu Liyuan, Lin Manman, Li Xue, et al.. Raman spectroscopy analysis of single white blood cell of DM mouse in vivo[J]. Laser & Optoelectronics Progress, 2012, 49(6): 063001.

    [22] [22] I Notingher, S Verrier, S Haque, et al.. Spectroscopic study of human lung epithelial cells (A549) in culture: Living cells versus dead cells[J]. Biopolymers, 2003, 72(4): 230-240.

    [23] [23] Jing Mingyan, Sun Jianyi. Protein folding and the formation of inclusion bodies[J]. Journal of Zhejiang University, 2004, 30(6): 690-696.

    [24] [24] Zhu Hongyu, Li Qiang. Strategies for expression of soluble heterologous proteins in Escherichia coli[J]. The Chinese Journal of Process Engineering, 2006, 6(1): 150-155.

    [25] [25] R S Donovan, C Robinson, B Glick. Review: Optimizing inducer and culture conditions for expression of foreign proteins under the control of the lac promoter[J]. J Ind Microbiol, 1996, 16(3): 145-154.

    [26] [26] P Carter, R F Kelley, M L Rodrigues, et al.. High level Escherichia coli expression and production of a bivalent humanized antibody fragment[J]. Nat Biotechnol, 1992, 10(2): 163-167.

    [27] [27] Fang Min, Huang Hualiang. Advances in in vitro refolding of inclusion body proteins[J]. Chinese Journal of Biotechnology, 2001, 17(6): 608-612.

    [28] [28] D Ami, A Natalello, P Gatti-Lafranconi, et al.. Kinetics of inclusion body formation studied in intact cells by FT-IR spectroscopy[J]. FEBS Lett, 2005, 579(16): 3433-3436

    [29] [29] M K Shaw, J L Ingraham. Synthesis of macromolecules by Escherichia coli near the minimal temperature for growth[J]. J Bacteriol, 1967, 94(1): 157-164.

    [30] [30] Wang Huimin. Raman Spectroscopic Studies of Human Stomach Tissues and the Interaction of Fibrinogen with Spermine[D]. Shanghai: East China Normal University, 2006. 27-29.

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    Huang Shushi, Lu Mingqian, Li Bing, Zhou Bing, Chen Limei. Real-Time Detection on the Expression of Soluble Protein and Inclusion Body in the Recombinant Escherichia coli with Laser Tweezers Raman Spectroscopy[J]. Chinese Journal of Lasers, 2014, 41(12): 1215003

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    Paper Information

    Category: Spectroscopy

    Received: Jun. 19, 2014

    Accepted: --

    Published Online: Oct. 30, 2014

    The Author Email: Shushi Huang (hshushi@gxas.cn)

    DOI:10.3788/cjl201441.1215003

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