Experiment Science and Technology, Volume. 23, Issue 3, 72(2025)
Experimental Design and Implementation of Direct Determination of Odorous Substances by Headspace Solid Phase Microextraction Combined with Gas Chromatography-Mass Spectrometry
Figures & Tables(7)
Fig. 1. Schematic diagram of the headspace solid-phase micro-extraction（HP-SPME）combined with GC-MS technique
Fig. 2. Chromatogram of 9 odorous substances under the selective ion mode
Fig. 3. Peak areas of 9 odor substances at different extraction temperatures
Fig. 4. Peak areas of 9 odor substances at different extraction times
Fig. 5. Experiments and criteria of quality control and assurance experiments
Table 1. Qualitative and quantitative parameters of individual compounds
View tableView in ArticleTable 1. Qualitative and quantitative parameters of individual compounds
化合物 英文缩写 保留时间/min 定量离子/（m·z−1） 定性离子/（m·z−1） 2-异丙基-3-甲氧基吡嗪 IPMP 9.39 136.7 150.7 2-异丁基-3-甲氧基吡嗪 IBMP 11.03 123.7 150.7 2-甲基异冰片 2-MIB 11.12 94.7 107.3 β-环柠檬醛 β-cyclocitral 11.82 136.7 151.7 2,4,6-三氯苯甲醚 2,4,6-TCA 13.72 209.5 211.4 2,3,6-三氯苯甲醚 2,3,6-TCA 14.46 209.5 211.4 土臭素 Geosmin 15.02 111.5 125.3 β-紫罗兰酮 β-ionone 16.28 177.0 134.9 2,3,4-三氯苯甲醚 2,3,4-TCA 16.55 209.5 211.4
Table 2. Standard curve, correlation coefficient, detection limit, average recovery rate and relative standard deviation（RSD）of 9 of odor substance（n = 6）
View tableView in ArticleTable 2. Standard curve, correlation coefficient, detection limit, average recovery rate and relative standard deviation（RSD）of 9 of odor substance（n = 6）
化合物 回归方程 R2 检测限/（ng·L−1） 平均回收率/% RSD/% IPMP y = 180.8x + 397.5 0.9996 2.00 95.4 4.5 IBMP y = 242.2x + 1388 0.9997 0.63 93.6 3.6 2-MIB y = 131.3x + 1010 0.9982 0.36 94.2 4.7 β-cyclocitral y = 54.47x + 516.0 0.9999 10.00 93.4 5.4 2,4,6-TCA y = 41.68x − 63.59 0.9995 15.50 98.3 6.4 2,3,6-TCA y = 77.47x + 1119 0.9998 2.78 97.7 8.5 Geosmin y = 193.1x + 237.7 0.9988 2.48 95.5 8.0 β-ionone y = 149.5x + 2265 0.9970 0.93 92.3 6.8 2,3,4-TCA y = 60.83x + 131.7 0.9959 6.29 88.7 6.6
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Junxia WANG, Guangli YAN, Youyi WU, Feiyue QIAN. Experimental Design and Implementation of Direct Determination of Odorous Substances by Headspace Solid Phase Microextraction Combined with Gas Chromatography-Mass Spectrometry[J]. Experiment Science and Technology, 2025, 23(3): 72
Paper Information
Category: Experimental Teaching and Teaching Experimental
Received: Apr. 1, 2024
Accepted: Sep. 10, 2024
Published Online: Jun. 9, 2025
The Author Email: Junxia WANG (wangjunxia@usts.edu.cn)
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