Acta Optica Sinica, Volume. 43, Issue 18, 1812002(2023)
Microfluidics-Microfluorescence-Based Method for Density Detection of Planktonic Algal Cells
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Fig. 1. Schematic diagram of microfluidic microfluorescence planktonic algae counting system
Fig. 5. Comparison of algal cell density under low-density microscopy and microfluidic microfluorescence detection. (a) Dunaliella salina; (b) Heterosigma akashiwo; (c) Cryptoalgae; (d) Chromococcus
Fig. 6. Comparison of algal cell density under high-density microscopy and microfluidic microfluorescence detection. (a) Dunaliella salina; (b) Heterosigma akashiwo; (c) Cryptoalgae; (d) Chromococcus
Fig. 7. Scatter plot of the relationship between relative error and cell density in the detection of four types of algae
Table 1. Comparison test results of the three methods
View tableTable 1. Comparison test results of the three methods
Detection method 1st /L-1 2nd /L-1 3rd /L-1 Average /L-1 RSD /% ARE /% Microscopic 1.752×108 1.545×108 1.677×108 1.658×108 6.30 3.49 Kurt counting 1.488×108 1.533×108 1.677×108 1.566×108 6.32 2.14 This system 1.650×108 1.550×108 1.600×108 1.600×108 3.12
Table 2. Experimental algal species information
View tableTable 2. Experimental algal species information
Algal Phylum Algal cell width /µm Dunaliella salina Chlorophyta 18-28 Cryptoalgae Cryptophyta 10-16 Heterosigma akashiwo Xanthophyta 6-10 Chromococcus Cyanophyta <5
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Peng Huang, Gaofang Yin, Nanjing Zhao, Tingting Gan, Xiang Hu, Min Xu, Tianhong Liang, Renqing Jia, Xiaoling Zhang. Microfluidics-Microfluorescence-Based Method for Density Detection of Planktonic Algal Cells[J]. Acta Optica Sinica, 2023, 43(18): 1812002
Paper Information
Category: Instrumentation, Measurement and Metrology
Received: Nov. 16, 2022
Accepted: Feb. 6, 2023
Published Online: Sep. 11, 2023
The Author Email: Yin Gaofang (gfyin@aiofm.ac.cn)
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