Fig. 1. Microfluidic channels.（a）Cross-section；（b）Schematic；（c）Physical image. x，y and z coordinates indicate the flow direction，the channel width direction and the channel depth direction，respectively.

Fig. 2. Planar alignment microchannel.（a）Director field distribution diagram in yz-plane；（b）POM texture in xy-plane.

Fig. 3. Homeotropic alignment microchannel.（a）Director field distribution diagram in yz-plane；（b）POM texture in xy-plane.

Fig. 4. Hybrid alignment microchannel.（a）Director field distribution diagram in yz-plane（width：40 μm）；（b）POM texture in xy-plane（width：40 μm）；（c）Director field distribution diagram in yz-plane（width：60 μm）；（d）POM texture in xy-plane（width：60 μm）.

Fig. 5. Flow in hybrid alignment microchannel.（a）Microchannel with 40 μm width；（b）Microchannel with 60 μm width.

Fig. 6. Schematic diagram of the evolution of defect lines

Fig. 7. Defect lines and defect wall

Fig. 8. Controlling the number of defect walls by changing the flow rate magnitude

Fig. 9. Disturbance phenomenon of the defect wall